High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.48797/sl.2023.77 |
Resumo: | Background: Escherichia coli is currently a leading pathogen for deaths associated with antimicrobial resistance (AMR) [1]. AMR related to food-producing animals is a public health risk requiring a One Health approach [1,2]. Animals are major reservoirs of antibiotic resistant E. coli which can easily reach humans through the food chain, direct contact, or the environment [3]. Objective: To analyze the AMR profiles of E. coli in fecal samples from Holstein-Friesian cattle, as information on AMR from dairy farming in Portugal is scarce. Methods: Samples (n=112) collected from 7 different farms at Northern Portugal were pooled during February-March 2023 based on age group (8 calves and 8 cows per farm). Characteristic E. coli colonies were selected from MacConkey Agar supplemented with or without antibiotics (4 µg/ml cefotaxime; 3 µg/ml colistin), for confirmation by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing (AST), according to EUCAST/CLSI guidelines. ESBL phenotype were searched using the Double-Disk Synergy Test. Results: Presumptive E. coli isolates (n=110) representing different farming production systems were obtained and 51 representatives were identified by MALDI-TOF and tested by AST. From both calves and cows, 95% and 63% of the E. coli isolates exhibited resistance to ≥1 antimicrobial classes and multidrug resistance (MDR, resistance to ≥3 antimicrobial classes), respectively. Isolates were mostly resistant to gentamycin (88%), ampicillin (72%), amoxicillin plus clavulanic acid (63%), tetracycline (63%) and cefotaxime (53%). Only two isolates showed susceptibility two all tested antibiotics. ESBL activity was observed in 21% of E. coli isolates. All analyzed pools were classified as MDR. Conclusions: This is one of the first studies on AMR rates of E. coli from dairy cattle in Northern Portugal. It reveals a high prevalence of MDR E. coli, which is a worrying finding and emphasize the need of a multisectoral One Health approach to minimize its impact. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challengePosterBackground: Escherichia coli is currently a leading pathogen for deaths associated with antimicrobial resistance (AMR) [1]. AMR related to food-producing animals is a public health risk requiring a One Health approach [1,2]. Animals are major reservoirs of antibiotic resistant E. coli which can easily reach humans through the food chain, direct contact, or the environment [3]. Objective: To analyze the AMR profiles of E. coli in fecal samples from Holstein-Friesian cattle, as information on AMR from dairy farming in Portugal is scarce. Methods: Samples (n=112) collected from 7 different farms at Northern Portugal were pooled during February-March 2023 based on age group (8 calves and 8 cows per farm). Characteristic E. coli colonies were selected from MacConkey Agar supplemented with or without antibiotics (4 µg/ml cefotaxime; 3 µg/ml colistin), for confirmation by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing (AST), according to EUCAST/CLSI guidelines. ESBL phenotype were searched using the Double-Disk Synergy Test. Results: Presumptive E. coli isolates (n=110) representing different farming production systems were obtained and 51 representatives were identified by MALDI-TOF and tested by AST. From both calves and cows, 95% and 63% of the E. coli isolates exhibited resistance to ≥1 antimicrobial classes and multidrug resistance (MDR, resistance to ≥3 antimicrobial classes), respectively. Isolates were mostly resistant to gentamycin (88%), ampicillin (72%), amoxicillin plus clavulanic acid (63%), tetracycline (63%) and cefotaxime (53%). Only two isolates showed susceptibility two all tested antibiotics. ESBL activity was observed in 21% of E. coli isolates. All analyzed pools were classified as MDR. Conclusions: This is one of the first studies on AMR rates of E. coli from dairy cattle in Northern Portugal. It reveals a high prevalence of MDR E. coli, which is a worrying finding and emphasize the need of a multisectoral One Health approach to minimize its impact.IUCS-CESPU Publishing2023-04-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2023.77https://doi.org/10.48797/sl.2023.77Scientific Letters; Vol. 1 No. Sup 1 (2023)2795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/77https://publicacoes.cespu.pt/index.php/sl/article/view/77/20Copyright (c) 2023 S. Quinteira , A. B. Dias , L. Pinho , N. V. Brito , C. Campos , A. R. Freitas , C. Mirandainfo:eu-repo/semantics/openAccessQuinteira , S.Dias , A. B.Pinho , L.Brito , N. V.Campos , C.Freitas , A. R.Miranda , C.2023-04-29T08:46:09Zoai:publicacoes.cespu.pt:article/77Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:50:23.488064Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
title |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
spellingShingle |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge Quinteira , S. Poster |
title_short |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
title_full |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
title_fullStr |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
title_full_unstemmed |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
title_sort |
High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge |
author |
Quinteira , S. |
author_facet |
Quinteira , S. Dias , A. B. Pinho , L. Brito , N. V. Campos , C. Freitas , A. R. Miranda , C. |
author_role |
author |
author2 |
Dias , A. B. Pinho , L. Brito , N. V. Campos , C. Freitas , A. R. Miranda , C. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Quinteira , S. Dias , A. B. Pinho , L. Brito , N. V. Campos , C. Freitas , A. R. Miranda , C. |
dc.subject.por.fl_str_mv |
Poster |
topic |
Poster |
description |
Background: Escherichia coli is currently a leading pathogen for deaths associated with antimicrobial resistance (AMR) [1]. AMR related to food-producing animals is a public health risk requiring a One Health approach [1,2]. Animals are major reservoirs of antibiotic resistant E. coli which can easily reach humans through the food chain, direct contact, or the environment [3]. Objective: To analyze the AMR profiles of E. coli in fecal samples from Holstein-Friesian cattle, as information on AMR from dairy farming in Portugal is scarce. Methods: Samples (n=112) collected from 7 different farms at Northern Portugal were pooled during February-March 2023 based on age group (8 calves and 8 cows per farm). Characteristic E. coli colonies were selected from MacConkey Agar supplemented with or without antibiotics (4 µg/ml cefotaxime; 3 µg/ml colistin), for confirmation by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing (AST), according to EUCAST/CLSI guidelines. ESBL phenotype were searched using the Double-Disk Synergy Test. Results: Presumptive E. coli isolates (n=110) representing different farming production systems were obtained and 51 representatives were identified by MALDI-TOF and tested by AST. From both calves and cows, 95% and 63% of the E. coli isolates exhibited resistance to ≥1 antimicrobial classes and multidrug resistance (MDR, resistance to ≥3 antimicrobial classes), respectively. Isolates were mostly resistant to gentamycin (88%), ampicillin (72%), amoxicillin plus clavulanic acid (63%), tetracycline (63%) and cefotaxime (53%). Only two isolates showed susceptibility two all tested antibiotics. ESBL activity was observed in 21% of E. coli isolates. All analyzed pools were classified as MDR. Conclusions: This is one of the first studies on AMR rates of E. coli from dairy cattle in Northern Portugal. It reveals a high prevalence of MDR E. coli, which is a worrying finding and emphasize the need of a multisectoral One Health approach to minimize its impact. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.48797/sl.2023.77 https://doi.org/10.48797/sl.2023.77 |
url |
https://doi.org/10.48797/sl.2023.77 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://publicacoes.cespu.pt/index.php/sl/article/view/77 https://publicacoes.cespu.pt/index.php/sl/article/view/77/20 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
dc.source.none.fl_str_mv |
Scientific Letters; Vol. 1 No. Sup 1 (2023) 2795-5117 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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