Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/538 |
Resumo: | A detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid. |
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Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannelMicrocirculationConfocal micro-PIVNipkow discBlood cell suspensionMicrochannelA detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid.Institut of Physics PublishingBiblioteca Digital do IPBLima, Rui A.Wada, ShigeoTsubota, Ken-ichiYamaguchi, Takami2008-02-26T17:47:56Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/538engengLima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2006). Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel. Measurement Science and Technology. ISSN 0957-0233. 17:4, p.797–808.0957-0233Measurement Science and Technology10.1088/0957-0233/17/4/026info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:31Zoai:bibliotecadigital.ipb.pt:10198/538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:18.451632Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
title |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
spellingShingle |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel Lima, Rui A. Microcirculation Confocal micro-PIV Nipkow disc Blood cell suspension Microchannel |
title_short |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
title_full |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
title_fullStr |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
title_full_unstemmed |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
title_sort |
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel |
author |
Lima, Rui A. |
author_facet |
Lima, Rui A. Wada, Shigeo Tsubota, Ken-ichi Yamaguchi, Takami |
author_role |
author |
author2 |
Wada, Shigeo Tsubota, Ken-ichi Yamaguchi, Takami |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Lima, Rui A. Wada, Shigeo Tsubota, Ken-ichi Yamaguchi, Takami |
dc.subject.por.fl_str_mv |
Microcirculation Confocal micro-PIV Nipkow disc Blood cell suspension Microchannel |
topic |
Microcirculation Confocal micro-PIV Nipkow disc Blood cell suspension Microchannel |
description |
A detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z 2008-02-26T17:47:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/538 |
url |
http://hdl.handle.net/10198/538 |
dc.language.iso.fl_str_mv |
eng eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2006). Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel. Measurement Science and Technology. ISSN 0957-0233. 17:4, p.797–808. 0957-0233 Measurement Science and Technology 10.1088/0957-0233/17/4/026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institut of Physics Publishing |
publisher.none.fl_str_mv |
Institut of Physics Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817552807390085120 |