Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel

Detalhes bibliográficos
Autor(a) principal: Lima, Rui A.
Data de Publicação: 2006
Outros Autores: Wada, Shigeo, Tsubota, Ken-ichi, Yamaguchi, Takami
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/538
Resumo: A detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid.
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spelling Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannelMicrocirculationConfocal micro-PIVNipkow discBlood cell suspensionMicrochannelA detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid.Institut of Physics PublishingBiblioteca Digital do IPBLima, Rui A.Wada, ShigeoTsubota, Ken-ichiYamaguchi, Takami2008-02-26T17:47:56Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/538engengLima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2006). Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel. Measurement Science and Technology. ISSN 0957-0233. 17:4, p.797–808.0957-0233Measurement Science and Technology10.1088/0957-0233/17/4/026info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:31Zoai:bibliotecadigital.ipb.pt:10198/538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:18.451632Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
title Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
spellingShingle Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
Lima, Rui A.
Microcirculation
Confocal micro-PIV
Nipkow disc
Blood cell suspension
Microchannel
title_short Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
title_full Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
title_fullStr Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
title_full_unstemmed Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
title_sort Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
author Lima, Rui A.
author_facet Lima, Rui A.
Wada, Shigeo
Tsubota, Ken-ichi
Yamaguchi, Takami
author_role author
author2 Wada, Shigeo
Tsubota, Ken-ichi
Yamaguchi, Takami
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Lima, Rui A.
Wada, Shigeo
Tsubota, Ken-ichi
Yamaguchi, Takami
dc.subject.por.fl_str_mv Microcirculation
Confocal micro-PIV
Nipkow disc
Blood cell suspension
Microchannel
topic Microcirculation
Confocal micro-PIV
Nipkow disc
Blood cell suspension
Microchannel
description A detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 μm square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s−1 in a blood cell suspension fluid.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2008-02-26T17:47:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/538
url http://hdl.handle.net/10198/538
dc.language.iso.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv Lima, R.; Wada, S.; Tsubota, K.; Yamaguchi, T. (2006). Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel. Measurement Science and Technology. ISSN 0957-0233. 17:4, p.797–808.
0957-0233
Measurement Science and Technology
10.1088/0957-0233/17/4/026
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Institut of Physics Publishing
publisher.none.fl_str_mv Institut of Physics Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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