Epigenetic reprogramming and small RNA silencing of transposable elements in pollen

Detalhes bibliográficos
Autor(a) principal: Slotkin, R.K.
Data de Publicação: 2009
Outros Autores: Vaughn, M., Borges, F., Tanurdzic, M., Becker, J.D., Feijó, J.A., Martienssen, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/67
Resumo: The mutagenic activity of transposable elements (TEs) is suppressed by epigenetic silencing and small interfering RNAs (siRNAs), especially in gametes that could transmit transposed elements to the next generation. In pollen from the model plant Arabidopsis, we show that TEs are unexpectedly reactivated and transpose, but only in the pollen vegetative nucleus, which accompanies the sperm cells but does not provide DNA to the fertilized zygote. TE expression coincides with downregulation of the heterochromatin remodeler decrease in DNA methylation 1 and of many TE siRNAs. However, 21 nucleotide siRNAs from Athila retrotransposons are generated and accumulate in pollen and sperm, suggesting that siRNA from TEs activated in the vegetative nucleus can target silencing in gametes. We propose a conserved role for reprogramming in germline companion cells, such as nurse cells in insects and vegetative nuclei in plants, to reveal intact TEs in the genome and regulate their activity in gametes
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spelling Epigenetic reprogramming and small RNA silencing of transposable elements in pollenArabidopsis/geneticsEpigenesis, GeneticPollen/geneticsRNA InterferenceThe mutagenic activity of transposable elements (TEs) is suppressed by epigenetic silencing and small interfering RNAs (siRNAs), especially in gametes that could transmit transposed elements to the next generation. In pollen from the model plant Arabidopsis, we show that TEs are unexpectedly reactivated and transpose, but only in the pollen vegetative nucleus, which accompanies the sperm cells but does not provide DNA to the fertilized zygote. TE expression coincides with downregulation of the heterochromatin remodeler decrease in DNA methylation 1 and of many TE siRNAs. However, 21 nucleotide siRNAs from Athila retrotransposons are generated and accumulate in pollen and sperm, suggesting that siRNA from TEs activated in the vegetative nucleus can target silencing in gametes. We propose a conserved role for reprogramming in germline companion cells, such as nurse cells in insects and vegetative nuclei in plants, to reveal intact TEs in the genome and regulate their activity in gametesElsevierARCASlotkin, R.K.Vaughn, M.Borges, F.Tanurdzic, M.Becker, J.D.Feijó, J.A.Martienssen, R.2010-03-18T10:14:22Z2009-02-062009-02-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/67engSlotkin RK, Vaughn M, Borges F, Tanurdžić M, Becker JD, Feijó JA, Martienssen R: Epigenetic reprogramming and small RNA silencing of transposable elements in pollen. Cell 136: 461–472 (2009)1097-4172info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:36Zoai:arca.igc.gulbenkian.pt:10400.7/67Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:33.527479Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
title Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
spellingShingle Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
Slotkin, R.K.
Arabidopsis/genetics
Epigenesis, Genetic
Pollen/genetics
RNA Interference
title_short Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
title_full Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
title_fullStr Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
title_full_unstemmed Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
title_sort Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
author Slotkin, R.K.
author_facet Slotkin, R.K.
Vaughn, M.
Borges, F.
Tanurdzic, M.
Becker, J.D.
Feijó, J.A.
Martienssen, R.
author_role author
author2 Vaughn, M.
Borges, F.
Tanurdzic, M.
Becker, J.D.
Feijó, J.A.
Martienssen, R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Slotkin, R.K.
Vaughn, M.
Borges, F.
Tanurdzic, M.
Becker, J.D.
Feijó, J.A.
Martienssen, R.
dc.subject.por.fl_str_mv Arabidopsis/genetics
Epigenesis, Genetic
Pollen/genetics
RNA Interference
topic Arabidopsis/genetics
Epigenesis, Genetic
Pollen/genetics
RNA Interference
description The mutagenic activity of transposable elements (TEs) is suppressed by epigenetic silencing and small interfering RNAs (siRNAs), especially in gametes that could transmit transposed elements to the next generation. In pollen from the model plant Arabidopsis, we show that TEs are unexpectedly reactivated and transpose, but only in the pollen vegetative nucleus, which accompanies the sperm cells but does not provide DNA to the fertilized zygote. TE expression coincides with downregulation of the heterochromatin remodeler decrease in DNA methylation 1 and of many TE siRNAs. However, 21 nucleotide siRNAs from Athila retrotransposons are generated and accumulate in pollen and sperm, suggesting that siRNA from TEs activated in the vegetative nucleus can target silencing in gametes. We propose a conserved role for reprogramming in germline companion cells, such as nurse cells in insects and vegetative nuclei in plants, to reveal intact TEs in the genome and regulate their activity in gametes
publishDate 2009
dc.date.none.fl_str_mv 2009-02-06
2009-02-06T00:00:00Z
2010-03-18T10:14:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/67
url http://hdl.handle.net/10400.7/67
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Slotkin RK, Vaughn M, Borges F, Tanurdžić M, Becker JD, Feijó JA, Martienssen R: Epigenetic reprogramming and small RNA silencing of transposable elements in pollen. Cell 136: 461–472 (2009)
1097-4172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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