rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts

Detalhes bibliográficos
Autor(a) principal: Sousa, Susana
Data de Publicação: 2020
Outros Autores: Almeida, André, Delgado, Lurdes, Conceição, Antónia, Marques, Cláudia, da Costa, José Manuel Correia, Castro, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7624
Resumo: Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.
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spelling rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocystsAmino Acid SequenceBiomarkersModels, MolecularOocystsProtein ConformationProtozoan ProteinsToxoplasmaInfecções Sistémicas e ZoonosesToxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.This research was supported by the Horizon 2020 project SafeConsume (Grant Agreement No. 727580), and FCT funding UIDB/00211/2020.Nature ResearchRepositório Científico do Instituto Nacional de SaúdeSousa, SusanaAlmeida, AndréDelgado, LurdesConceição, AntóniaMarques, Cláudiada Costa, José Manuel CorreiaCastro, António2021-04-01T14:39:28Z2020-05-142020-05-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7624engSci Rep. 2020 May 14;10(1):7947. doi: 10.1038/s41598-020-64590-42045-232210.1038/s41598-020-64590-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:07Zoai:repositorio.insa.pt:10400.18/7624Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:15.343850Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
spellingShingle rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
Sousa, Susana
Amino Acid Sequence
Biomarkers
Models, Molecular
Oocysts
Protein Conformation
Protozoan Proteins
Toxoplasma
Infecções Sistémicas e Zoonoses
title_short rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_full rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_fullStr rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_full_unstemmed rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
title_sort rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
author Sousa, Susana
author_facet Sousa, Susana
Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
author_role author
author2 Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Sousa, Susana
Almeida, André
Delgado, Lurdes
Conceição, Antónia
Marques, Cláudia
da Costa, José Manuel Correia
Castro, António
dc.subject.por.fl_str_mv Amino Acid Sequence
Biomarkers
Models, Molecular
Oocysts
Protein Conformation
Protozoan Proteins
Toxoplasma
Infecções Sistémicas e Zoonoses
topic Amino Acid Sequence
Biomarkers
Models, Molecular
Oocysts
Protein Conformation
Protozoan Proteins
Toxoplasma
Infecções Sistémicas e Zoonoses
description Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-14
2020-05-14T00:00:00Z
2021-04-01T14:39:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7624
url http://hdl.handle.net/10400.18/7624
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sci Rep. 2020 May 14;10(1):7947. doi: 10.1038/s41598-020-64590-4
2045-2322
10.1038/s41598-020-64590-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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