rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/7624 |
Resumo: | Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification. |
id |
RCAP_f054fa35877450e3f706fb44f49f250e |
---|---|
oai_identifier_str |
oai:repositorio.insa.pt:10400.18/7624 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocystsAmino Acid SequenceBiomarkersModels, MolecularOocystsProtein ConformationProtozoan ProteinsToxoplasmaInfecções Sistémicas e ZoonosesToxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.This research was supported by the Horizon 2020 project SafeConsume (Grant Agreement No. 727580), and FCT funding UIDB/00211/2020.Nature ResearchRepositório Científico do Instituto Nacional de SaúdeSousa, SusanaAlmeida, AndréDelgado, LurdesConceição, AntóniaMarques, Cláudiada Costa, José Manuel CorreiaCastro, António2021-04-01T14:39:28Z2020-05-142020-05-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7624engSci Rep. 2020 May 14;10(1):7947. doi: 10.1038/s41598-020-64590-42045-232210.1038/s41598-020-64590-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:07Zoai:repositorio.insa.pt:10400.18/7624Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:15.343850Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
title |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
spellingShingle |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts Sousa, Susana Amino Acid Sequence Biomarkers Models, Molecular Oocysts Protein Conformation Protozoan Proteins Toxoplasma Infecções Sistémicas e Zoonoses |
title_short |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
title_full |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
title_fullStr |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
title_full_unstemmed |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
title_sort |
rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts |
author |
Sousa, Susana |
author_facet |
Sousa, Susana Almeida, André Delgado, Lurdes Conceição, Antónia Marques, Cláudia da Costa, José Manuel Correia Castro, António |
author_role |
author |
author2 |
Almeida, André Delgado, Lurdes Conceição, Antónia Marques, Cláudia da Costa, José Manuel Correia Castro, António |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Sousa, Susana Almeida, André Delgado, Lurdes Conceição, Antónia Marques, Cláudia da Costa, José Manuel Correia Castro, António |
dc.subject.por.fl_str_mv |
Amino Acid Sequence Biomarkers Models, Molecular Oocysts Protein Conformation Protozoan Proteins Toxoplasma Infecções Sistémicas e Zoonoses |
topic |
Amino Acid Sequence Biomarkers Models, Molecular Oocysts Protein Conformation Protozoan Proteins Toxoplasma Infecções Sistémicas e Zoonoses |
description |
Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-14 2020-05-14T00:00:00Z 2021-04-01T14:39:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7624 |
url |
http://hdl.handle.net/10400.18/7624 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sci Rep. 2020 May 14;10(1):7947. doi: 10.1038/s41598-020-64590-4 2045-2322 10.1038/s41598-020-64590-4 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132167282884608 |