Interleukin-27 early impacts Leishmania infantum infection in mice and correlates with active visceral disease in humans

• Autor(a) principal: Pérez-Cabezas, Begoña
• Data de Publicação: 2016
• Outros Autores: Cecílio, Pedro, Robalo, Ana Luísa, Silvestre, Ricardo Jorge Leal, Carrillo, Eugenia, Moreno, Javier, Martín, Juan V. San, Vasconcellos, Rita, Silva, Anabela Cordeiro da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/44896
• topic: IL-27; Leishmania infantum; Human; Mouse models; Immune regulation; Ciências Médicas::Medicina Básica; Science & Technology

The complexity of Leishmania host interactions, one of the main leishmaniasis issues, is yet to be fully understood. We detected elevated IL-27 plasma levels in European patients with active visceral disease caused by Leishmania infantum, which returned to basal levels after successful treatment, suggesting this cytokine as a probable infection mediator. We further addressed this hypothesis recurring to two classical susceptible visceral leishmaniasis mouse models. BALB/c, but not C57BU6 mice, showed increased IL-27 systemic levels after infection, which was associated with an upregulation of IL-27p28 expression by dendritic cells and higher parasite burdens. Neutralization of IL-27 in acutely infected BALB/c led to decreased parasite burdens and a transient increase in IFN-gamma(+) splenic T cells, while administration of IL-27 to C57BU6 promoted a local anti-inflammatory cytokine response at the site of infection and increased parasite loads. Overall, we show that, as in humans, BALB/c IL-27 systemic levels are infection dependently upregulated and may favor parasite installation by controlling inflammation.