Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model

Detalhes bibliográficos
Autor(a) principal: Casimiro, Maria Helena
Data de Publicação: 2022
Outros Autores: Ferreira, Luís M., Santos, Pedro M. P., Leal, João P., Rodrigues, Gabriela, Iria, Inês, Alves, Sara, Pais, Diogo, Casal, Diogo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/55569
Resumo: Frequently, deep partial and full-thickness skin wounds do not spontaneously regenerate. To restore the normal function of skin, epidermal and dermal components have to be supplied to the wound bed by grafting various substrates. Available options are limited and frequently costly. Herein, authors present a possible approach using 3D skin scaffolds capable of mimicking structure and biological functions of the extracellular matrix, providing, in parallel, a good environment for cell attachment, proliferation and differentiation. Low-molecular weight chitosan-based membranes were prepared by freeze-drying and ionizing radiation techniques to be used as skin scaffolds. Poly (vinyl alcohol), PVA, vinyl pyrrolidone, VP, and gelatin from cold water fish were incorporated. Information regarding membranes’ physical-chemical properties from SEM analysis, swelling and weight loss, together with biological response through in vitro assays (using Human Caucasian Fetal Foreskin Fibroblast) allowed the selection of an optimized batch of membranes that was used as skin scaffold in a dorsal rat model wound. The in vivo implantation assays (in Wistar rats) resulted in very promising results: (i) healing process faster than control; (ii) good vascularization; (iii) viable new tissues morphologically functional.
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spelling Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat ModelFrequently, deep partial and full-thickness skin wounds do not spontaneously regenerate. To restore the normal function of skin, epidermal and dermal components have to be supplied to the wound bed by grafting various substrates. Available options are limited and frequently costly. Herein, authors present a possible approach using 3D skin scaffolds capable of mimicking structure and biological functions of the extracellular matrix, providing, in parallel, a good environment for cell attachment, proliferation and differentiation. Low-molecular weight chitosan-based membranes were prepared by freeze-drying and ionizing radiation techniques to be used as skin scaffolds. Poly (vinyl alcohol), PVA, vinyl pyrrolidone, VP, and gelatin from cold water fish were incorporated. Information regarding membranes’ physical-chemical properties from SEM analysis, swelling and weight loss, together with biological response through in vitro assays (using Human Caucasian Fetal Foreskin Fibroblast) allowed the selection of an optimized batch of membranes that was used as skin scaffold in a dorsal rat model wound. The in vivo implantation assays (in Wistar rats) resulted in very promising results: (i) healing process faster than control; (ii) good vascularization; (iii) viable new tissues morphologically functional.MDPIRepositório da Universidade de LisboaCasimiro, Maria HelenaFerreira, Luís M.Santos, Pedro M. P.Leal, João P.Rodrigues, GabrielaIria, InêsAlves, SaraPais, DiogoCasal, Diogo2023-01-03T10:16:16Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/55569engCasimiro, M.H.; Ferreira, L.M.; Santos, P.M.P.; Leal, J.P.; Rodrigues, G.; Iria, I.; Alves, S.; Pais, D.; Casal, D. Chitosan-Based Membranes for SkinWound Repair in a Dorsal Fold Chamber Rat Model. Pharmaceutics 2022, 14, 2736. https://doi.org/10.3390/ pharmaceutics1412273610.3390/pharmaceutics14122736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:02:38Zoai:repositorio.ul.pt:10451/55569Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:13.327536Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
spellingShingle Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
Casimiro, Maria Helena
title_short Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_full Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_fullStr Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_full_unstemmed Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
title_sort Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
author Casimiro, Maria Helena
author_facet Casimiro, Maria Helena
Ferreira, Luís M.
Santos, Pedro M. P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
author_role author
author2 Ferreira, Luís M.
Santos, Pedro M. P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Casimiro, Maria Helena
Ferreira, Luís M.
Santos, Pedro M. P.
Leal, João P.
Rodrigues, Gabriela
Iria, Inês
Alves, Sara
Pais, Diogo
Casal, Diogo
description Frequently, deep partial and full-thickness skin wounds do not spontaneously regenerate. To restore the normal function of skin, epidermal and dermal components have to be supplied to the wound bed by grafting various substrates. Available options are limited and frequently costly. Herein, authors present a possible approach using 3D skin scaffolds capable of mimicking structure and biological functions of the extracellular matrix, providing, in parallel, a good environment for cell attachment, proliferation and differentiation. Low-molecular weight chitosan-based membranes were prepared by freeze-drying and ionizing radiation techniques to be used as skin scaffolds. Poly (vinyl alcohol), PVA, vinyl pyrrolidone, VP, and gelatin from cold water fish were incorporated. Information regarding membranes’ physical-chemical properties from SEM analysis, swelling and weight loss, together with biological response through in vitro assays (using Human Caucasian Fetal Foreskin Fibroblast) allowed the selection of an optimized batch of membranes that was used as skin scaffold in a dorsal rat model wound. The in vivo implantation assays (in Wistar rats) resulted in very promising results: (i) healing process faster than control; (ii) good vascularization; (iii) viable new tissues morphologically functional.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-01-03T10:16:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/55569
url http://hdl.handle.net/10451/55569
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Casimiro, M.H.; Ferreira, L.M.; Santos, P.M.P.; Leal, J.P.; Rodrigues, G.; Iria, I.; Alves, S.; Pais, D.; Casal, D. Chitosan-Based Membranes for SkinWound Repair in a Dorsal Fold Chamber Rat Model. Pharmaceutics 2022, 14, 2736. https://doi.org/10.3390/ pharmaceutics14122736
10.3390/pharmaceutics14122736
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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