Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/147552 |
Resumo: | Funding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors. |
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Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Modelcell proliferationchitosangamma radiationgelatinPVAregenerative medicineskin scaffoldsVPPharmaceutical ScienceFunding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors.Frequently, deep partial and full-thickness skin wounds do not spontaneously regenerate. To restore the normal function of skin, epidermal and dermal components have to be supplied to the wound bed by grafting various substrates. Available options are limited and frequently costly. Herein, authors present a possible approach using 3D skin scaffolds capable of mimicking structure and biological functions of the extracellular matrix, providing, in parallel, a good environment for cell attachment, proliferation and differentiation. Low-molecular weight chitosan-based membranes were prepared by freeze-drying and ionizing radiation techniques to be used as skin scaffolds. Poly (vinyl alcohol), PVA, vinyl pyrrolidone, VP, and gelatin from cold water fish were incorporated. Information regarding membranes’ physical-chemical properties from SEM analysis, swelling and weight loss, together with biological response through in vitro assays (using Human Caucasian Fetal Foreskin Fibroblast) allowed the selection of an optimized batch of membranes that was used as skin scaffold in a dorsal rat model wound. The in vivo implantation assays (in Wistar rats) resulted in very promising results: (i) healing process faster than control; (ii) good vascularization; (iii) viable new tissues morphologically functional.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNCasimiro, Maria HelenaFerreira, Luís M.Santos, Pedro M.P.Leal, João P.Rodrigues, GabrielaIria, InêsAlves, SaraPais, DiogoCasal, Diogo2023-01-13T22:20:12Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147552eng1999-4923PURE: 49547609https://doi.org/10.3390/pharmaceutics14122736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:08:09Zoai:run.unl.pt:10362/147552Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:08:09Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| title |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| spellingShingle |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model Casimiro, Maria Helena cell proliferation chitosan gamma radiation gelatin PVA regenerative medicine skin scaffolds VP Pharmaceutical Science |
| title_short |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| title_full |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| title_fullStr |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| title_full_unstemmed |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| title_sort |
Chitosan-Based Membranes for Skin Wound Repair in a Dorsal Fold Chamber Rat Model |
| author |
Casimiro, Maria Helena |
| author_facet |
Casimiro, Maria Helena Ferreira, Luís M. Santos, Pedro M.P. Leal, João P. Rodrigues, Gabriela Iria, Inês Alves, Sara Pais, Diogo Casal, Diogo |
| author_role |
author |
| author2 |
Ferreira, Luís M. Santos, Pedro M.P. Leal, João P. Rodrigues, Gabriela Iria, Inês Alves, Sara Pais, Diogo Casal, Diogo |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
| dc.contributor.author.fl_str_mv |
Casimiro, Maria Helena Ferreira, Luís M. Santos, Pedro M.P. Leal, João P. Rodrigues, Gabriela Iria, Inês Alves, Sara Pais, Diogo Casal, Diogo |
| dc.subject.por.fl_str_mv |
cell proliferation chitosan gamma radiation gelatin PVA regenerative medicine skin scaffolds VP Pharmaceutical Science |
| topic |
cell proliferation chitosan gamma radiation gelatin PVA regenerative medicine skin scaffolds VP Pharmaceutical Science |
| description |
Funding Information: This research was funded by the International Atomic Energy Agency under Research Contract No. 18202 (CRP F23030). Authors acknowledge FCT—Fundação para a Ciência e a Tecnologia I.P. for the national funds in the scope of the project UIDB/04349/2020+UIDP/04349/2020, UIDB/00100/2020 and UIDB/00329/2020. Publisher Copyright: © 2022 by the authors. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-12 2022-12-01T00:00:00Z 2023-01-13T22:20:12Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/147552 |
| url |
http://hdl.handle.net/10362/147552 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1999-4923 PURE: 49547609 https://doi.org/10.3390/pharmaceutics14122736 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545909304557568 |