Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies

Detalhes bibliográficos
Autor(a) principal: Zabala, L.
Data de Publicação: 1999
Outros Autores: Saldanha, C., Martins e Silva, J., Souza-Ramalho, P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/17892
Resumo: This work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998
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spelling Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studiesAcetylcholinesteraseGlaucomaPilocarpineRed blood cellTimololThis work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998© 1999 Royal College of OphthalmologistsPurpose There is increasing evidence that abnormal perfusion of the optic nerve head is an important factor involved in the pathophysiology of glaucoma. Transport and distribution of oxygen to the tissues takes place through the erythrocyte membrane. Red blood cell (RBC) acetylcholinesterase (AChE) is a marker of RBC membrane integrity. The aim of this study was to find out whether RBC membrane integrity is preserved in primary open angle glaucoma (POAG), and whether it is modified by the use of topical timolol maleate and pilocarpine. Method RBC AChE activity was determined ex vivo by Kaplan's spectrophotometric method in 19 POAG patients undergoing topical treatment for glaucoma with timolol, pilocarpine or a combination of the two drugs, and compared with that in 20 controls. To assess the effect of antiglaucomatous therapy in our findings, we carried out an in vitro study in 26 non-glaucomatous patients in which we measured RBC AChE activity after incubation of blood with either timolol maleate, pilocarpine or a combination of the two drugs, using the same spectrophotometric method. Results There was a significant increase in RBC AChE enzyme activity in POAG patients compared with the control group (p < 0.002). However, timolol and pilocarpine, individually or in combination, have the opposite effect, significantly decreasing RBC AChE activity (p < 0.05). Conclusion This change in RBC AChE enzyme activity could suggest alterations in RBC membrane integrity in primary open angle glaucoma. Whether or not this finding has implications regarding the microcirculation at the optic nerve head needs to be investigated further.Royal College of OphthalmologistsRepositório da Universidade de LisboaZabala, L.Saldanha, C.Martins e Silva, J.Souza-Ramalho, P.2015-04-14T10:37:35Z19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17892engEye (1999) 13, 101-1030950-222Xhttp://www.nature.com/eye/journal/v13/n1/abs/eye199918a.htmlmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:03:47Zoai:repositorio.ul.pt:10451/17892Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:37:36.736893Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
title Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
spellingShingle Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
Zabala, L.
Acetylcholinesterase
Glaucoma
Pilocarpine
Red blood cell
Timolol
title_short Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
title_full Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
title_fullStr Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
title_full_unstemmed Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
title_sort Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
author Zabala, L.
author_facet Zabala, L.
Saldanha, C.
Martins e Silva, J.
Souza-Ramalho, P.
author_role author
author2 Saldanha, C.
Martins e Silva, J.
Souza-Ramalho, P.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Zabala, L.
Saldanha, C.
Martins e Silva, J.
Souza-Ramalho, P.
dc.subject.por.fl_str_mv Acetylcholinesterase
Glaucoma
Pilocarpine
Red blood cell
Timolol
topic Acetylcholinesterase
Glaucoma
Pilocarpine
Red blood cell
Timolol
description This work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01T00:00:00Z
2015-04-14T10:37:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/17892
url http://hdl.handle.net/10451/17892
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eye (1999) 13, 101-103
0950-222X
http://www.nature.com/eye/journal/v13/n1/abs/eye199918a.html
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal College of Ophthalmologists
publisher.none.fl_str_mv Royal College of Ophthalmologists
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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