Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/17892 |
Resumo: | This work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998 |
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7160 |
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Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studiesAcetylcholinesteraseGlaucomaPilocarpineRed blood cellTimololThis work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998© 1999 Royal College of OphthalmologistsPurpose There is increasing evidence that abnormal perfusion of the optic nerve head is an important factor involved in the pathophysiology of glaucoma. Transport and distribution of oxygen to the tissues takes place through the erythrocyte membrane. Red blood cell (RBC) acetylcholinesterase (AChE) is a marker of RBC membrane integrity. The aim of this study was to find out whether RBC membrane integrity is preserved in primary open angle glaucoma (POAG), and whether it is modified by the use of topical timolol maleate and pilocarpine. Method RBC AChE activity was determined ex vivo by Kaplan's spectrophotometric method in 19 POAG patients undergoing topical treatment for glaucoma with timolol, pilocarpine or a combination of the two drugs, and compared with that in 20 controls. To assess the effect of antiglaucomatous therapy in our findings, we carried out an in vitro study in 26 non-glaucomatous patients in which we measured RBC AChE activity after incubation of blood with either timolol maleate, pilocarpine or a combination of the two drugs, using the same spectrophotometric method. Results There was a significant increase in RBC AChE enzyme activity in POAG patients compared with the control group (p < 0.002). However, timolol and pilocarpine, individually or in combination, have the opposite effect, significantly decreasing RBC AChE activity (p < 0.05). Conclusion This change in RBC AChE enzyme activity could suggest alterations in RBC membrane integrity in primary open angle glaucoma. Whether or not this finding has implications regarding the microcirculation at the optic nerve head needs to be investigated further.Royal College of OphthalmologistsRepositório da Universidade de LisboaZabala, L.Saldanha, C.Martins e Silva, J.Souza-Ramalho, P.2015-04-14T10:37:35Z19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17892engEye (1999) 13, 101-1030950-222Xhttp://www.nature.com/eye/journal/v13/n1/abs/eye199918a.htmlmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:03:47Zoai:repositorio.ul.pt:10451/17892Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:37:36.736893Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
title |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
spellingShingle |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies Zabala, L. Acetylcholinesterase Glaucoma Pilocarpine Red blood cell Timolol |
title_short |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
title_full |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
title_fullStr |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
title_full_unstemmed |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
title_sort |
Red blood cell membrane integrity in primary open angle glaucoma : ex vivo and in vitro studies |
author |
Zabala, L. |
author_facet |
Zabala, L. Saldanha, C. Martins e Silva, J. Souza-Ramalho, P. |
author_role |
author |
author2 |
Saldanha, C. Martins e Silva, J. Souza-Ramalho, P. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Zabala, L. Saldanha, C. Martins e Silva, J. Souza-Ramalho, P. |
dc.subject.por.fl_str_mv |
Acetylcholinesterase Glaucoma Pilocarpine Red blood cell Timolol |
topic |
Acetylcholinesterase Glaucoma Pilocarpine Red blood cell Timolol |
description |
This work was presented as a poster at the Tenth Anniversary Congress of the Royal College of Ophthalmologists, Glasgow 1998 |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 1999-01-01T00:00:00Z 2015-04-14T10:37:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/17892 |
url |
http://hdl.handle.net/10451/17892 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eye (1999) 13, 101-103 0950-222X http://www.nature.com/eye/journal/v13/n1/abs/eye199918a.html |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal College of Ophthalmologists |
publisher.none.fl_str_mv |
Royal College of Ophthalmologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134273897234432 |