Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/19915 |
Resumo: | The knowledge of the optical properties of biological tissues in a wide spectral range is highly important for the development of noninvasive diagnostic or treatment procedures. The absorption coefficient is one of those properties, from which various information about tissue components can be retrieved. Using transmittance and reflectance spectral measurements acquired from ex vivo rabbit brain cortex samples allowed to calculate its optical properties in the ultraviolet to the near infrared spectral range. Melanin and lipofuscin, the two pigments that are related to the aging of tissues and cells were identified in the cortex absorption. By subtracting the absorption of these pigments from the absorption of the brain cortex, it was possible to evaluate the true ratios for the DNA/RNA and hemoglobin bands in the cortex—12.33-fold (at 260 nm), 12.02-fold (at 411 nm) and 4.47-fold (at 555 nm). Since melanin and lipofuscin accumulation increases with the aging of the brain tissues and are related to the degeneration of neurons and their death, further studies should be performed to evaluate the evolution of pigment accumulation in the brain, so that new optical methods can be developed to aid in the diagnosis and monitoring of brain diseases. |
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Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nmTissue spectroscopyTissue optical propertiesScattering coefficientAbsorption coefficientDNA contentBlood contentPigment detectionThe knowledge of the optical properties of biological tissues in a wide spectral range is highly important for the development of noninvasive diagnostic or treatment procedures. The absorption coefficient is one of those properties, from which various information about tissue components can be retrieved. Using transmittance and reflectance spectral measurements acquired from ex vivo rabbit brain cortex samples allowed to calculate its optical properties in the ultraviolet to the near infrared spectral range. Melanin and lipofuscin, the two pigments that are related to the aging of tissues and cells were identified in the cortex absorption. By subtracting the absorption of these pigments from the absorption of the brain cortex, it was possible to evaluate the true ratios for the DNA/RNA and hemoglobin bands in the cortex—12.33-fold (at 260 nm), 12.02-fold (at 411 nm) and 4.47-fold (at 555 nm). Since melanin and lipofuscin accumulation increases with the aging of the brain tissues and are related to the degeneration of neurons and their death, further studies should be performed to evaluate the evolution of pigment accumulation in the brain, so that new optical methods can be developed to aid in the diagnosis and monitoring of brain diseases.The work of L.M.O. was supported by the Portuguese Science Foundation, Grant No. FCT-UIDB/04730/2020. The work of V.V.T. was supported by the Government of the Russian Federation (project No. 075-15-2019-1885 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions, Russian institutions of higher education).MDPIRepositório Científico do Instituto Politécnico do PortoGonçalves, Tânia M.Martins, InêsSilva, HugoTuchin, Valery V.Oliveira, Luís2022-02-11T10:42:47Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdftext/plain; charset=utf-8http://hdl.handle.net/10400.22/19915engGonçalves, T.M., Martins, I.S., Silva, H.F., Tuchin, V.V., Oliveira, L.M. Spectral optical properties of rabbit brain cortex between 200 and 1000 nm. Photochem, vol. 1, no. 2, 190-208.10.3390/photochem1020011info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:14:48Zoai:recipp.ipp.pt:10400.22/19915Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:39:58.422459Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
title |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
spellingShingle |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm Gonçalves, Tânia M. Tissue spectroscopy Tissue optical properties Scattering coefficient Absorption coefficient DNA content Blood content Pigment detection |
title_short |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
title_full |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
title_fullStr |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
title_full_unstemmed |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
title_sort |
Spectral Optical Properties of Rabbit Brain Cortex between 200 and 1000 nm |
author |
Gonçalves, Tânia M. |
author_facet |
Gonçalves, Tânia M. Martins, Inês Silva, Hugo Tuchin, Valery V. Oliveira, Luís |
author_role |
author |
author2 |
Martins, Inês Silva, Hugo Tuchin, Valery V. Oliveira, Luís |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Gonçalves, Tânia M. Martins, Inês Silva, Hugo Tuchin, Valery V. Oliveira, Luís |
dc.subject.por.fl_str_mv |
Tissue spectroscopy Tissue optical properties Scattering coefficient Absorption coefficient DNA content Blood content Pigment detection |
topic |
Tissue spectroscopy Tissue optical properties Scattering coefficient Absorption coefficient DNA content Blood content Pigment detection |
description |
The knowledge of the optical properties of biological tissues in a wide spectral range is highly important for the development of noninvasive diagnostic or treatment procedures. The absorption coefficient is one of those properties, from which various information about tissue components can be retrieved. Using transmittance and reflectance spectral measurements acquired from ex vivo rabbit brain cortex samples allowed to calculate its optical properties in the ultraviolet to the near infrared spectral range. Melanin and lipofuscin, the two pigments that are related to the aging of tissues and cells were identified in the cortex absorption. By subtracting the absorption of these pigments from the absorption of the brain cortex, it was possible to evaluate the true ratios for the DNA/RNA and hemoglobin bands in the cortex—12.33-fold (at 260 nm), 12.02-fold (at 411 nm) and 4.47-fold (at 555 nm). Since melanin and lipofuscin accumulation increases with the aging of the brain tissues and are related to the degeneration of neurons and their death, further studies should be performed to evaluate the evolution of pigment accumulation in the brain, so that new optical methods can be developed to aid in the diagnosis and monitoring of brain diseases. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-02-11T10:42:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/19915 |
url |
http://hdl.handle.net/10400.22/19915 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gonçalves, T.M., Martins, I.S., Silva, H.F., Tuchin, V.V., Oliveira, L.M. Spectral optical properties of rabbit brain cortex between 200 and 1000 nm. Photochem, vol. 1, no. 2, 190-208. 10.3390/photochem1020011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/plain; charset=utf-8 |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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