Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116722 |
Resumo: | Funding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infectionBabesia bigeminaRhipicephalus annulatusApoptosisProteomicsStress responseTranscriptomicsVector–pathogen interactionsParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.A system biology approach was used to gain insight into tick biology and interactions between vector and pathogen. Rhipicephalus annulatus is one of the main vectors of Babesia bigemina which has a massive impact on animal health. It is vital to obtain more information about this relationship, to better understand tick and pathogen biology, pathogen transmission dynamics, and new potential control approaches. In ticks, salivary glands (SGs) play a key role during pathogen infection and transmission. RNA sequencing obtained from uninfected and B. bigemina infected SGs obtained from fed female ticks resulted in 6823 and 6475 unigenes, respectively. From these, 360 unigenes were found to be differentially expressed (p < 0.05). Reversed phase liquid chromatography-mass spectrometry identified a total of 3679 tick proteins. Among them 406 were differently represented in response to Babesia infection. The omics data obtained suggested that Babesia infection lead to a reduction in the levels of mRNA and proteins (n = 237 transcripts, n = 212 proteins) when compared to uninfected controls. Integrated transcriptomics and proteomics datasets suggested a key role for stress response and apoptosis pathways in response to infection. Thus, six genes coding for GP80, death-associated protein kinase (DAPK-1), bax inhibitor-1 related (BI-1), heat shock protein (HSP), heat shock transcription factor (PHSTF), and queuine trna-ribosyltransferase (QtRibosyl) were selected and RNA interference (RNAi) performed. Gene silencing was obtained for all genes except phstf. Knockdown of gp80, dapk-1, and bi-1 led to a significant increase in Babesia infection levels while hsp and QtRibosyl knockdown resulted in a non-significant decrease of infection levels when compared to the respective controls. Gene knockdown did not affect tick survival, but engorged female weight and egg production were affected in the gp80, dapk-1, and QtRibosyl-silenced groups in comparison to controls. These results advanced our understanding of tick-Babesia molecular interactions, and suggested new tick antigens as putative targets for vaccination to control tick infestations and pathogen infection/transmission.Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)RUNAntunes, SCouto, JoanaFerrolho, JSeron, GustavoCharrez, José Octavio Merinode la Cruz-Hernández, Ned IvánMazuz, Monica L.Villar, MargaritaShkap, Varda A.de la Fuente, José LuisDomingos, A2021-05-02T22:45:06Z2019-04-022019-04-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/116722eng1664-042XPURE: 14976253https://doi.org/10.3389/fphys.2019.00318info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:26Zoai:run.unl.pt:10362/116722Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:14.035666Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
title |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
spellingShingle |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection Antunes, S Babesia bigemina Rhipicephalus annulatus Apoptosis Proteomics Stress response Transcriptomics Vector–pathogen interactions Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
title_full |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
title_fullStr |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
title_full_unstemmed |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
title_sort |
Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection |
author |
Antunes, S |
author_facet |
Antunes, S Couto, Joana Ferrolho, J Seron, Gustavo Charrez, José Octavio Merino de la Cruz-Hernández, Ned Iván Mazuz, Monica L. Villar, Margarita Shkap, Varda A. de la Fuente, José Luis Domingos, A |
author_role |
author |
author2 |
Couto, Joana Ferrolho, J Seron, Gustavo Charrez, José Octavio Merino de la Cruz-Hernández, Ned Iván Mazuz, Monica L. Villar, Margarita Shkap, Varda A. de la Fuente, José Luis Domingos, A |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
dc.contributor.author.fl_str_mv |
Antunes, S Couto, Joana Ferrolho, J Seron, Gustavo Charrez, José Octavio Merino de la Cruz-Hernández, Ned Iván Mazuz, Monica L. Villar, Margarita Shkap, Varda A. de la Fuente, José Luis Domingos, A |
dc.subject.por.fl_str_mv |
Babesia bigemina Rhipicephalus annulatus Apoptosis Proteomics Stress response Transcriptomics Vector–pathogen interactions Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Babesia bigemina Rhipicephalus annulatus Apoptosis Proteomics Stress response Transcriptomics Vector–pathogen interactions Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Funding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-02 2019-04-02T00:00:00Z 2021-05-02T22:45:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116722 |
url |
http://hdl.handle.net/10362/116722 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-042X PURE: 14976253 https://doi.org/10.3389/fphys.2019.00318 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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17 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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