Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection

Detalhes bibliográficos
Autor(a) principal: Antunes, S
Data de Publicação: 2019
Outros Autores: Couto, Joana, Ferrolho, J, Seron, Gustavo, Charrez, José Octavio Merino, de la Cruz-Hernández, Ned Iván, Mazuz, Monica L., Villar, Margarita, Shkap,  Varda A., de la Fuente, José Luis, Domingos, A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116722
Resumo: Funding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
id RCAP_f166c4e60da82210009a5391b010f3c4
oai_identifier_str oai:run.unl.pt:10362/116722
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infectionBabesia bigeminaRhipicephalus annulatusApoptosisProteomicsStress responseTranscriptomicsVector–pathogen interactionsParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.A system biology approach was used to gain insight into tick biology and interactions between vector and pathogen. Rhipicephalus annulatus is one of the main vectors of Babesia bigemina which has a massive impact on animal health. It is vital to obtain more information about this relationship, to better understand tick and pathogen biology, pathogen transmission dynamics, and new potential control approaches. In ticks, salivary glands (SGs) play a key role during pathogen infection and transmission. RNA sequencing obtained from uninfected and B. bigemina infected SGs obtained from fed female ticks resulted in 6823 and 6475 unigenes, respectively. From these, 360 unigenes were found to be differentially expressed (p < 0.05). Reversed phase liquid chromatography-mass spectrometry identified a total of 3679 tick proteins. Among them 406 were differently represented in response to Babesia infection. The omics data obtained suggested that Babesia infection lead to a reduction in the levels of mRNA and proteins (n = 237 transcripts, n = 212 proteins) when compared to uninfected controls. Integrated transcriptomics and proteomics datasets suggested a key role for stress response and apoptosis pathways in response to infection. Thus, six genes coding for GP80, death-associated protein kinase (DAPK-1), bax inhibitor-1 related (BI-1), heat shock protein (HSP), heat shock transcription factor (PHSTF), and queuine trna-ribosyltransferase (QtRibosyl) were selected and RNA interference (RNAi) performed. Gene silencing was obtained for all genes except phstf. Knockdown of gp80, dapk-1, and bi-1 led to a significant increase in Babesia infection levels while hsp and QtRibosyl knockdown resulted in a non-significant decrease of infection levels when compared to the respective controls. Gene knockdown did not affect tick survival, but engorged female weight and egg production were affected in the gp80, dapk-1, and QtRibosyl-silenced groups in comparison to controls. These results advanced our understanding of tick-Babesia molecular interactions, and suggested new tick antigens as putative targets for vaccination to control tick infestations and pathogen infection/transmission.Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)RUNAntunes, SCouto, JoanaFerrolho, JSeron, GustavoCharrez, José Octavio Merinode la Cruz-Hernández, Ned IvánMazuz, Monica L.Villar, MargaritaShkap,  Varda A.de la Fuente, José LuisDomingos, A2021-05-02T22:45:06Z2019-04-022019-04-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/116722eng1664-042XPURE: 14976253https://doi.org/10.3389/fphys.2019.00318info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:26Zoai:run.unl.pt:10362/116722Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:14.035666Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
title Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
spellingShingle Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
Antunes, S
Babesia bigemina
Rhipicephalus annulatus
Apoptosis
Proteomics
Stress response
Transcriptomics
Vector–pathogen interactions
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
title_full Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
title_fullStr Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
title_full_unstemmed Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
title_sort Transcriptome and proteome response of Rhipicephalus annulatus tick vector to Babesia bigemina infection
author Antunes, S
author_facet Antunes, S
Couto, Joana
Ferrolho, J
Seron, Gustavo
Charrez, José Octavio Merino
de la Cruz-Hernández, Ned Iván
Mazuz, Monica L.
Villar, Margarita
Shkap,  Varda A.
de la Fuente, José Luis
Domingos, A
author_role author
author2 Couto, Joana
Ferrolho, J
Seron, Gustavo
Charrez, José Octavio Merino
de la Cruz-Hernández, Ned Iván
Mazuz, Monica L.
Villar, Margarita
Shkap,  Varda A.
de la Fuente, José Luis
Domingos, A
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Antunes, S
Couto, Joana
Ferrolho, J
Seron, Gustavo
Charrez, José Octavio Merino
de la Cruz-Hernández, Ned Iván
Mazuz, Monica L.
Villar, Margarita
Shkap,  Varda A.
de la Fuente, José Luis
Domingos, A
dc.subject.por.fl_str_mv Babesia bigemina
Rhipicephalus annulatus
Apoptosis
Proteomics
Stress response
Transcriptomics
Vector–pathogen interactions
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Babesia bigemina
Rhipicephalus annulatus
Apoptosis
Proteomics
Stress response
Transcriptomics
Vector–pathogen interactions
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding Information: RHIBAB - PTDC/CVT/112050/2009 “Differential expression and functional characterization of tick (Rhipicephalus annulatus) genes in response to pathogen infection (B. bigemina).” SA is the recipient of a post-doctoral grant supported by FCT Funding Information: The authors would like to acknowledge Fundação para a Ciência e Tecnologia (FCT) for funds to GHTM – UID/Multi/04413/2013. Publisher Copyright: Copyright © 2019 Antunes, Couto, Ferrolho, Sanches, Merino Charrez, De la Cruz Hernández, Mazuz, Villar, Shkap, de la Fuente and Domingos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-02
2019-04-02T00:00:00Z
2021-05-02T22:45:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116722
url http://hdl.handle.net/10362/116722
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-042X
PURE: 14976253
https://doi.org/10.3389/fphys.2019.00318
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138042548584448