Electrochemical aptasensor array for multiple detection of human osteopontin

Detalhes bibliográficos
Autor(a) principal: Meirinho, Sofia G.
Data de Publicação: 2018
Outros Autores: Dias, L.G., Peres, António M., Rodrigues, Lígia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/15375
Resumo: Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.
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spelling Electrochemical aptasensor array for multiple detection of human osteopontinAptamerAptasensor arrayDual-screen-printed gold electrodeElectrochemical detectionOsteopontinElectrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.The authors acknowledge the financial support from the Strategic funding of UID/BIO/04469/2013 and from project ref. RECI/BBB-EBI/ 0179/2012 (project number FCOMP-01-0124-FEDER-027462). This work was also financially supported by Project POCI-01–0145-FEDER-006984 – Associate Laboratory LSRE-LCM and by Project UID/QUI/00616/2013 – CQ-VR, both funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia, Portugal. S. Meirinho also acknowledges the doctoral grant (ref SFRH/BD/65021/2009) funded by FCT.Biblioteca Digital do IPBMeirinho, Sofia G.Dias, L.G.Peres, António M.Rodrigues, Lígia R.2018-01-31T10:00:00Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15375engMeirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2018). Electrochemical aptasensor array for multiple detection of human osteopontin. Portugaliae Electrochimica Acta. ISSN 0872-1904. 36, p. 1-90872-190410.4152/pea.201801001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:57Zoai:bibliotecadigital.ipb.pt:10198/15375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:58.975129Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Electrochemical aptasensor array for multiple detection of human osteopontin
title Electrochemical aptasensor array for multiple detection of human osteopontin
spellingShingle Electrochemical aptasensor array for multiple detection of human osteopontin
Meirinho, Sofia G.
Aptamer
Aptasensor array
Dual-screen-printed gold electrode
Electrochemical detection
Osteopontin
title_short Electrochemical aptasensor array for multiple detection of human osteopontin
title_full Electrochemical aptasensor array for multiple detection of human osteopontin
title_fullStr Electrochemical aptasensor array for multiple detection of human osteopontin
title_full_unstemmed Electrochemical aptasensor array for multiple detection of human osteopontin
title_sort Electrochemical aptasensor array for multiple detection of human osteopontin
author Meirinho, Sofia G.
author_facet Meirinho, Sofia G.
Dias, L.G.
Peres, António M.
Rodrigues, Lígia R.
author_role author
author2 Dias, L.G.
Peres, António M.
Rodrigues, Lígia R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Meirinho, Sofia G.
Dias, L.G.
Peres, António M.
Rodrigues, Lígia R.
dc.subject.por.fl_str_mv Aptamer
Aptasensor array
Dual-screen-printed gold electrode
Electrochemical detection
Osteopontin
topic Aptamer
Aptasensor array
Dual-screen-printed gold electrode
Electrochemical detection
Osteopontin
description Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-31T10:00:00Z
2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/15375
url http://hdl.handle.net/10198/15375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2018). Electrochemical aptasensor array for multiple detection of human osteopontin. Portugaliae Electrochimica Acta. ISSN 0872-1904. 36, p. 1-9
0872-1904
10.4152/pea.201801001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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