Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/126529 |
Resumo: | Background: Noise is an important environmental risk factor. Industrial environments are rich in high-intensity infrasound (hi-IFS), which we have found to induce myocardial and coronary perivascular fibrosis in rats. The effects of exposure to IFS on the ventricles have been studied, but not on the atria. We hypothesized that rats exposed to hi-IFS develop atrial remodeling involving fibrosis and connexin 43, which we sought to evaluate. Material and methods: Seventy-two Wistar rats, half exposed to hi-IFS (120 dB, < 20 Hz) during a maximum period of 12 weeks and half age-matched controls, were studied. Atrial fibrosis was analyzed by Chromotropeaniline blue staining. The immunohistochemical evaluation of Cx43 was performed using the polyclonal antibody connexin-43 m diluted 1:1000 at 4 degrees C overnight. Digitized images were obtained with an optical microscope using 400 x magnifications. The measurements were performed using image J software. A two-way ANOVA model was used to compare the groups. Results: The mean values of the ratio "atrial fibrosis / cardiomyocytes" increased to a maximum of 0.1095 +/- 0,04 and 0.5408 +/- 0,01, and of the ratio "CX43 / cardiomyocytes" decreased to 0.0834 +/- 0,03 and 0.0966 +/- 0,03, respectively in IFS-exposed rats and controls. IFS-exposed rats exhibited a significantly higher ratio of fibrosis (p < .001) and lower ratio of Cx43 (p = .009). Conclusion: High-intensity infrasound exposure leads to an increase in atrial interstitial fibrosis and a decrease in connexin 43 in rat hearts. This finding reinforces the need for further experimental and clinical studies concerning the effects of exposure to infrasound. |
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Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasoundMedicina, Acústica, Outras ciências médicasMedicine, Acoustics, Other medical sciencesBackground: Noise is an important environmental risk factor. Industrial environments are rich in high-intensity infrasound (hi-IFS), which we have found to induce myocardial and coronary perivascular fibrosis in rats. The effects of exposure to IFS on the ventricles have been studied, but not on the atria. We hypothesized that rats exposed to hi-IFS develop atrial remodeling involving fibrosis and connexin 43, which we sought to evaluate. Material and methods: Seventy-two Wistar rats, half exposed to hi-IFS (120 dB, < 20 Hz) during a maximum period of 12 weeks and half age-matched controls, were studied. Atrial fibrosis was analyzed by Chromotropeaniline blue staining. The immunohistochemical evaluation of Cx43 was performed using the polyclonal antibody connexin-43 m diluted 1:1000 at 4 degrees C overnight. Digitized images were obtained with an optical microscope using 400 x magnifications. The measurements were performed using image J software. A two-way ANOVA model was used to compare the groups. Results: The mean values of the ratio "atrial fibrosis / cardiomyocytes" increased to a maximum of 0.1095 +/- 0,04 and 0.5408 +/- 0,01, and of the ratio "CX43 / cardiomyocytes" decreased to 0.0834 +/- 0,03 and 0.0966 +/- 0,03, respectively in IFS-exposed rats and controls. IFS-exposed rats exhibited a significantly higher ratio of fibrosis (p < .001) and lower ratio of Cx43 (p = .009). Conclusion: High-intensity infrasound exposure leads to an increase in atrial interstitial fibrosis and a decrease in connexin 43 in rat hearts. This finding reinforces the need for further experimental and clinical studies concerning the effects of exposure to infrasound.2020-062020-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/126529eng0014-480010.1016/j.yexmp.2020.104409António Oliveira de CarvalhoAna LousinhaGonçalo PereiraGonçalo BorrechoJosé BritoDiamantino FreitasPedro OliveiraMaria João R. OliveiraEduardo Antunesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T12:45:34Zoai:repositorio-aberto.up.pt:10216/126529Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:26:11.686619Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
title |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
spellingShingle |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound António Oliveira de Carvalho Medicina, Acústica, Outras ciências médicas Medicine, Acoustics, Other medical sciences |
title_short |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
title_full |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
title_fullStr |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
title_full_unstemmed |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
title_sort |
Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound |
author |
António Oliveira de Carvalho |
author_facet |
António Oliveira de Carvalho Ana Lousinha Gonçalo Pereira Gonçalo Borrecho José Brito Diamantino Freitas Pedro Oliveira Maria João R. Oliveira Eduardo Antunes |
author_role |
author |
author2 |
Ana Lousinha Gonçalo Pereira Gonçalo Borrecho José Brito Diamantino Freitas Pedro Oliveira Maria João R. Oliveira Eduardo Antunes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
António Oliveira de Carvalho Ana Lousinha Gonçalo Pereira Gonçalo Borrecho José Brito Diamantino Freitas Pedro Oliveira Maria João R. Oliveira Eduardo Antunes |
dc.subject.por.fl_str_mv |
Medicina, Acústica, Outras ciências médicas Medicine, Acoustics, Other medical sciences |
topic |
Medicina, Acústica, Outras ciências médicas Medicine, Acoustics, Other medical sciences |
description |
Background: Noise is an important environmental risk factor. Industrial environments are rich in high-intensity infrasound (hi-IFS), which we have found to induce myocardial and coronary perivascular fibrosis in rats. The effects of exposure to IFS on the ventricles have been studied, but not on the atria. We hypothesized that rats exposed to hi-IFS develop atrial remodeling involving fibrosis and connexin 43, which we sought to evaluate. Material and methods: Seventy-two Wistar rats, half exposed to hi-IFS (120 dB, < 20 Hz) during a maximum period of 12 weeks and half age-matched controls, were studied. Atrial fibrosis was analyzed by Chromotropeaniline blue staining. The immunohistochemical evaluation of Cx43 was performed using the polyclonal antibody connexin-43 m diluted 1:1000 at 4 degrees C overnight. Digitized images were obtained with an optical microscope using 400 x magnifications. The measurements were performed using image J software. A two-way ANOVA model was used to compare the groups. Results: The mean values of the ratio "atrial fibrosis / cardiomyocytes" increased to a maximum of 0.1095 +/- 0,04 and 0.5408 +/- 0,01, and of the ratio "CX43 / cardiomyocytes" decreased to 0.0834 +/- 0,03 and 0.0966 +/- 0,03, respectively in IFS-exposed rats and controls. IFS-exposed rats exhibited a significantly higher ratio of fibrosis (p < .001) and lower ratio of Cx43 (p = .009). Conclusion: High-intensity infrasound exposure leads to an increase in atrial interstitial fibrosis and a decrease in connexin 43 in rat hearts. This finding reinforces the need for further experimental and clinical studies concerning the effects of exposure to infrasound. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06 2020-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/126529 |
url |
https://hdl.handle.net/10216/126529 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0014-4800 10.1016/j.yexmp.2020.104409 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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