Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)

Detalhes bibliográficos
Autor(a) principal: Ruiz-Jarabo, I.
Data de Publicação: 2022
Outros Autores: Gregorio, Silvia, Fuentes, Xoan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/17970
Resumo: Calcium balance is of paramount importance for vertebrates. In fish, the endocrine modulators of calcium homeostasis include the stanniocalcin (STC), and some members of the parathyroid hormone (PTH) family, such as the PTH-related protein (PTHrP), acting as antagonists. STC is ubiquitously expressed in higher vertebrates. In turn, bony fish exhibit specific STC-producing glands named the corpuscles of Stannius (CS). Previous studies pointed to a calcium-sensing receptor (CaSR) involvement in the secretion of STC, but little is known of the involvement of other putative regulators. The CS provides a unique model to deepen the study of STC secretion. We developed an ex vivo assay to culture CS of fish and a competitive ELISA method to measure STC concentrations. As expected, STC released from the CS responds to CaSR stimulation by calcium, calcimimetics, and calcilytic drugs. Moreover, we uncover the presence (by PCR) of two PTHrP receptors in the CS, e.g., PTH1R and PTH3R. Thus, ex vivo incubations revealed a dose-response inhibition of STC secretion in response to PTHrP at basal Ca2+ concentrations. This inhibition is achieved through specific and reversible second messenger pathways (transmembrane adenylyl cyclases and phospholipase C), as the use of specific inhibitors highlights. Together, these results provide evidence for endocrine modulation between two antagonist hormones, STC and PTHrP.
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spelling Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)Calcium-sensing receptorCorpuscles of StanniusParathyroid hormone-related proteinStanniocalcinSparus aurataCalcium balance is of paramount importance for vertebrates. In fish, the endocrine modulators of calcium homeostasis include the stanniocalcin (STC), and some members of the parathyroid hormone (PTH) family, such as the PTH-related protein (PTHrP), acting as antagonists. STC is ubiquitously expressed in higher vertebrates. In turn, bony fish exhibit specific STC-producing glands named the corpuscles of Stannius (CS). Previous studies pointed to a calcium-sensing receptor (CaSR) involvement in the secretion of STC, but little is known of the involvement of other putative regulators. The CS provides a unique model to deepen the study of STC secretion. We developed an ex vivo assay to culture CS of fish and a competitive ELISA method to measure STC concentrations. As expected, STC released from the CS responds to CaSR stimulation by calcium, calcimimetics, and calcilytic drugs. Moreover, we uncover the presence (by PCR) of two PTHrP receptors in the CS, e.g., PTH1R and PTH3R. Thus, ex vivo incubations revealed a dose-response inhibition of STC secretion in response to PTHrP at basal Ca2+ concentrations. This inhibition is achieved through specific and reversible second messenger pathways (transmembrane adenylyl cyclases and phospholipase C), as the use of specific inhibitors highlights. Together, these results provide evidence for endocrine modulation between two antagonist hormones, STC and PTHrP.MDPISapientiaRuiz-Jarabo, I.Gregorio, SilviaFuentes, Xoan2022-07-12T12:22:06Z2022-06-042022-06-23T12:11:37Z2022-06-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/17970engBiology 11 (6): 863 (2022)10.3390/biology110608632079-7737info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:30:07Zoai:sapientia.ualg.pt:10400.1/17970Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:07:44.889162Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
title Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
spellingShingle Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
Ruiz-Jarabo, I.
Calcium-sensing receptor
Corpuscles of Stannius
Parathyroid hormone-related protein
Stanniocalcin
Sparus aurata
title_short Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
title_full Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
title_fullStr Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
title_full_unstemmed Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
title_sort Regulation of stanniocalcin secretion by calcium and PTHrP in Gilthead Seabream (Sparus aurata)
author Ruiz-Jarabo, I.
author_facet Ruiz-Jarabo, I.
Gregorio, Silvia
Fuentes, Xoan
author_role author
author2 Gregorio, Silvia
Fuentes, Xoan
author2_role author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Ruiz-Jarabo, I.
Gregorio, Silvia
Fuentes, Xoan
dc.subject.por.fl_str_mv Calcium-sensing receptor
Corpuscles of Stannius
Parathyroid hormone-related protein
Stanniocalcin
Sparus aurata
topic Calcium-sensing receptor
Corpuscles of Stannius
Parathyroid hormone-related protein
Stanniocalcin
Sparus aurata
description Calcium balance is of paramount importance for vertebrates. In fish, the endocrine modulators of calcium homeostasis include the stanniocalcin (STC), and some members of the parathyroid hormone (PTH) family, such as the PTH-related protein (PTHrP), acting as antagonists. STC is ubiquitously expressed in higher vertebrates. In turn, bony fish exhibit specific STC-producing glands named the corpuscles of Stannius (CS). Previous studies pointed to a calcium-sensing receptor (CaSR) involvement in the secretion of STC, but little is known of the involvement of other putative regulators. The CS provides a unique model to deepen the study of STC secretion. We developed an ex vivo assay to culture CS of fish and a competitive ELISA method to measure STC concentrations. As expected, STC released from the CS responds to CaSR stimulation by calcium, calcimimetics, and calcilytic drugs. Moreover, we uncover the presence (by PCR) of two PTHrP receptors in the CS, e.g., PTH1R and PTH3R. Thus, ex vivo incubations revealed a dose-response inhibition of STC secretion in response to PTHrP at basal Ca2+ concentrations. This inhibition is achieved through specific and reversible second messenger pathways (transmembrane adenylyl cyclases and phospholipase C), as the use of specific inhibitors highlights. Together, these results provide evidence for endocrine modulation between two antagonist hormones, STC and PTHrP.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-12T12:22:06Z
2022-06-04
2022-06-23T12:11:37Z
2022-06-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/17970
url http://hdl.handle.net/10400.1/17970
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biology 11 (6): 863 (2022)
10.3390/biology11060863
2079-7737
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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