Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/28511 |
Resumo: | Cell encapsulation systems must ensure the diffusion of molecules to avoid the formation of necrotic cores. The architectural design of hydrogels, the gold standard tissue engineering strategy, is thus limited to a microsize range. To overcome such a limitation, liquefied microcapsules encapsulating cells and microparticles are proposed. Microcapsules with controlled sizes with average diameters of 608.5 ± 122.3 µm are produced at high rates by electrohydrodynamic atomization, and arginyl-glycyl-aspartic acid (RGD) domains are introduced in the multilayered membrane. While cells and microparticles interact toward the production of confined microaggregates, on the outside cell-mediated macroaggregates are formed due to the aggregation of microcapsules. The concept of simultaneous aggregation is herein termed as 3D+3D bottom-up tissue engineering. Microcapsules are cultured alone (microcapsule1 ) or on top of 2D cell beds composed of human umbilical vein endothelial cells (HUVECs) alone (microcapsule2 ) or cocultured with fibroblasts (microcapsule3 ). Microcapsules are able to support cell encapsulation shown by LiveDead, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphofenyl)-2H-tetrazolium (MTS), and dsDNA assays. Only microcapsule3 are able to form macroaggregates, as shown by F-actin immunofluorescence. The bioactive 3D system also presented alkaline phosphatase activity, thus allowing osteogenic differentiation. Upon implantation using the chick chorioallontoic membrane (CAM) model, microcapsules recruit a similar number of vessels with alike geometric parameters in comparison with CAMs supplemented with basic fibroblast growth factor (bFGF). |
id |
RCAP_f342bb8bdd51b7b832ece88f56df826b |
---|---|
oai_identifier_str |
oai:ria.ua.pt:10773/28511 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineeringElectrosprayingLiquefied CapsulesTissue Engineering and Regenerative Medicine3D SystemsHydrogelsLayer-by-LayerMicroparticlesBottom-upRGDCell encapsulation systems must ensure the diffusion of molecules to avoid the formation of necrotic cores. The architectural design of hydrogels, the gold standard tissue engineering strategy, is thus limited to a microsize range. To overcome such a limitation, liquefied microcapsules encapsulating cells and microparticles are proposed. Microcapsules with controlled sizes with average diameters of 608.5 ± 122.3 µm are produced at high rates by electrohydrodynamic atomization, and arginyl-glycyl-aspartic acid (RGD) domains are introduced in the multilayered membrane. While cells and microparticles interact toward the production of confined microaggregates, on the outside cell-mediated macroaggregates are formed due to the aggregation of microcapsules. The concept of simultaneous aggregation is herein termed as 3D+3D bottom-up tissue engineering. Microcapsules are cultured alone (microcapsule1 ) or on top of 2D cell beds composed of human umbilical vein endothelial cells (HUVECs) alone (microcapsule2 ) or cocultured with fibroblasts (microcapsule3 ). Microcapsules are able to support cell encapsulation shown by LiveDead, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphofenyl)-2H-tetrazolium (MTS), and dsDNA assays. Only microcapsule3 are able to form macroaggregates, as shown by F-actin immunofluorescence. The bioactive 3D system also presented alkaline phosphatase activity, thus allowing osteogenic differentiation. Upon implantation using the chick chorioallontoic membrane (CAM) model, microcapsules recruit a similar number of vessels with alike geometric parameters in comparison with CAMs supplemented with basic fibroblast growth factor (bFGF).Wiley2020-11-01T00:00:00Z2019-11-01T00:00:00Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/28511eng2192-264010.1002/adhm.201901221Correia, Clara R.Bjorge, Isabel M.Zeng, JinfengMatsusaki, MichiyaMano, João F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:55:09Zoai:ria.ua.pt:10773/28511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:01:02.241159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
title |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
spellingShingle |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering Correia, Clara R. Electrospraying Liquefied Capsules Tissue Engineering and Regenerative Medicine 3D Systems Hydrogels Layer-by-Layer Microparticles Bottom-up RGD |
title_short |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
title_full |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
title_fullStr |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
title_full_unstemmed |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
title_sort |
Liquefied microcapsules as dual-Mmcrocarriers for 3D+3D bottom-up tissue engineering |
author |
Correia, Clara R. |
author_facet |
Correia, Clara R. Bjorge, Isabel M. Zeng, Jinfeng Matsusaki, Michiya Mano, João F. |
author_role |
author |
author2 |
Bjorge, Isabel M. Zeng, Jinfeng Matsusaki, Michiya Mano, João F. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Correia, Clara R. Bjorge, Isabel M. Zeng, Jinfeng Matsusaki, Michiya Mano, João F. |
dc.subject.por.fl_str_mv |
Electrospraying Liquefied Capsules Tissue Engineering and Regenerative Medicine 3D Systems Hydrogels Layer-by-Layer Microparticles Bottom-up RGD |
topic |
Electrospraying Liquefied Capsules Tissue Engineering and Regenerative Medicine 3D Systems Hydrogels Layer-by-Layer Microparticles Bottom-up RGD |
description |
Cell encapsulation systems must ensure the diffusion of molecules to avoid the formation of necrotic cores. The architectural design of hydrogels, the gold standard tissue engineering strategy, is thus limited to a microsize range. To overcome such a limitation, liquefied microcapsules encapsulating cells and microparticles are proposed. Microcapsules with controlled sizes with average diameters of 608.5 ± 122.3 µm are produced at high rates by electrohydrodynamic atomization, and arginyl-glycyl-aspartic acid (RGD) domains are introduced in the multilayered membrane. While cells and microparticles interact toward the production of confined microaggregates, on the outside cell-mediated macroaggregates are formed due to the aggregation of microcapsules. The concept of simultaneous aggregation is herein termed as 3D+3D bottom-up tissue engineering. Microcapsules are cultured alone (microcapsule1 ) or on top of 2D cell beds composed of human umbilical vein endothelial cells (HUVECs) alone (microcapsule2 ) or cocultured with fibroblasts (microcapsule3 ). Microcapsules are able to support cell encapsulation shown by LiveDead, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphofenyl)-2H-tetrazolium (MTS), and dsDNA assays. Only microcapsule3 are able to form macroaggregates, as shown by F-actin immunofluorescence. The bioactive 3D system also presented alkaline phosphatase activity, thus allowing osteogenic differentiation. Upon implantation using the chick chorioallontoic membrane (CAM) model, microcapsules recruit a similar number of vessels with alike geometric parameters in comparison with CAMs supplemented with basic fibroblast growth factor (bFGF). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01T00:00:00Z 2019-11-01 2020-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/28511 |
url |
http://hdl.handle.net/10773/28511 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2192-2640 10.1002/adhm.201901221 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137666181103616 |