Freeze-extraction microporous electroactive supports for cell culture
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/64783 |
Resumo: | Poly(vinylidene fluoride) (PVDF) is a semicrystalline polymer with four crystalline phases, of which the all trans conformation (β-phase) is the one with the largest piezoelectric response and best electroactive properties. This smart material is able to reproduce physiological events such as inherent bone piezoelectricity, making it a perfect candidate to drive the osteogenic differentiation of mesenchymal stem cells (MSCs) towards the osteogenic lineage. The influence of topography on the adhesion, proliferation and maintenance of multipotency of this type of cell is well established and has confirmed that the production of variable porosity substrates is a suitable approach for cell therapy. In this work, novel PVDF microporous membranes in the β-phase were developed by the freeze-extraction technique. Several concentrations of PVDF in N,N-dimethylformamide (10, 15 and 20% w/v) were used to obtain membranes with different grades of porosity in the range of 80–84%. The cell culture supports thus produced were found to possess good crystallinity (66%), β-phase presence (94%) and a microstructure based on spherulite agglomerations with a diameter of spherulite in the order of 1 μm that is higher as the polymer concentration increases. The membranes have good mechanical properties and the storage modulus, with values between 5 and 47 MPa, rises with the polymer content of the starting solution. Porcine bone marrow mesenchymal stem cells (pBM-MSCs) were used to study cell adhesion and proliferation. Regarding cell adhesion at 24 h, the cells preferred the more porous structures and had round focal adhesions with well-developed cytoskeletons, while they had a round morphology on the less porous membranes. The cells preferred the less porous membranes to proliferate, even though the initial morphology at 24 h showed poor adhesion. These findings confirm that the controlled microporosity of β-phase PVDF membranes can be produced by freeze extraction and offer the possibility of modifying the adhesion and proliferation of pBM-MSCs on these substrates. |
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Freeze-extraction microporous electroactive supports for cell culturePoly(vinylidene fluoride)Freeze extractionMesenchymal stem cellTissue engineeringCiências Naturais::Ciências FísicasScience & TechnologyPoly(vinylidene fluoride) (PVDF) is a semicrystalline polymer with four crystalline phases, of which the all trans conformation (β-phase) is the one with the largest piezoelectric response and best electroactive properties. This smart material is able to reproduce physiological events such as inherent bone piezoelectricity, making it a perfect candidate to drive the osteogenic differentiation of mesenchymal stem cells (MSCs) towards the osteogenic lineage. The influence of topography on the adhesion, proliferation and maintenance of multipotency of this type of cell is well established and has confirmed that the production of variable porosity substrates is a suitable approach for cell therapy. In this work, novel PVDF microporous membranes in the β-phase were developed by the freeze-extraction technique. Several concentrations of PVDF in N,N-dimethylformamide (10, 15 and 20% w/v) were used to obtain membranes with different grades of porosity in the range of 80–84%. The cell culture supports thus produced were found to possess good crystallinity (66%), β-phase presence (94%) and a microstructure based on spherulite agglomerations with a diameter of spherulite in the order of 1 μm that is higher as the polymer concentration increases. The membranes have good mechanical properties and the storage modulus, with values between 5 and 47 MPa, rises with the polymer content of the starting solution. Porcine bone marrow mesenchymal stem cells (pBM-MSCs) were used to study cell adhesion and proliferation. Regarding cell adhesion at 24 h, the cells preferred the more porous structures and had round focal adhesions with well-developed cytoskeletons, while they had a round morphology on the less porous membranes. The cells preferred the less porous membranes to proliferate, even though the initial morphology at 24 h showed poor adhesion. These findings confirm that the controlled microporosity of β-phase PVDF membranes can be produced by freeze extraction and offer the possibility of modifying the adhesion and proliferation of pBM-MSCs on these substrates.This work was supported by the Spanish Government through Projects MAT2016-76039-C4-1-R and MAT2016-76039-C4-3-R (including FEDER funds). Maria Guillot-Ferriols acknowledges the government funding of her doctoral thesis through a BES-2017-080398FPI Grant. The CIBER-BBN initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.Pergamon-Elsevier Science LtdUniversidade do MinhoMorales-Román, R. M.Guillot-Ferriols, M.Roig-Pérez, L.Lanceros-Méndez, S.Gallego-Ferrer, G.Gómez Ribelles, J. L.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/64783engMorales-Román, R. M., Guillot-Ferriols, M., Roig-Pérez, L., Lanceros-Mendez, S., Gallego-Ferrer, G., & Gómez Ribelles, J. L. (2019, October). Freeze-extraction microporous electroactive supports for cell culture. European Polymer Journal. Elsevier BV. http://doi.org/10.1016/j.eurpolymj.2019.07.0110014-30571873-194510.1016/j.eurpolymj.2019.07.011https://www.sciencedirect.com/science/article/pii/S0014305719306913info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:07Zoai:repositorium.sdum.uminho.pt:1822/64783Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:44.879990Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Freeze-extraction microporous electroactive supports for cell culture |
title |
Freeze-extraction microporous electroactive supports for cell culture |
spellingShingle |
Freeze-extraction microporous electroactive supports for cell culture Morales-Román, R. M. Poly(vinylidene fluoride) Freeze extraction Mesenchymal stem cell Tissue engineering Ciências Naturais::Ciências Físicas Science & Technology |
title_short |
Freeze-extraction microporous electroactive supports for cell culture |
title_full |
Freeze-extraction microporous electroactive supports for cell culture |
title_fullStr |
Freeze-extraction microporous electroactive supports for cell culture |
title_full_unstemmed |
Freeze-extraction microporous electroactive supports for cell culture |
title_sort |
Freeze-extraction microporous electroactive supports for cell culture |
author |
Morales-Román, R. M. |
author_facet |
Morales-Román, R. M. Guillot-Ferriols, M. Roig-Pérez, L. Lanceros-Méndez, S. Gallego-Ferrer, G. Gómez Ribelles, J. L. |
author_role |
author |
author2 |
Guillot-Ferriols, M. Roig-Pérez, L. Lanceros-Méndez, S. Gallego-Ferrer, G. Gómez Ribelles, J. L. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Morales-Román, R. M. Guillot-Ferriols, M. Roig-Pérez, L. Lanceros-Méndez, S. Gallego-Ferrer, G. Gómez Ribelles, J. L. |
dc.subject.por.fl_str_mv |
Poly(vinylidene fluoride) Freeze extraction Mesenchymal stem cell Tissue engineering Ciências Naturais::Ciências Físicas Science & Technology |
topic |
Poly(vinylidene fluoride) Freeze extraction Mesenchymal stem cell Tissue engineering Ciências Naturais::Ciências Físicas Science & Technology |
description |
Poly(vinylidene fluoride) (PVDF) is a semicrystalline polymer with four crystalline phases, of which the all trans conformation (β-phase) is the one with the largest piezoelectric response and best electroactive properties. This smart material is able to reproduce physiological events such as inherent bone piezoelectricity, making it a perfect candidate to drive the osteogenic differentiation of mesenchymal stem cells (MSCs) towards the osteogenic lineage. The influence of topography on the adhesion, proliferation and maintenance of multipotency of this type of cell is well established and has confirmed that the production of variable porosity substrates is a suitable approach for cell therapy. In this work, novel PVDF microporous membranes in the β-phase were developed by the freeze-extraction technique. Several concentrations of PVDF in N,N-dimethylformamide (10, 15 and 20% w/v) were used to obtain membranes with different grades of porosity in the range of 80–84%. The cell culture supports thus produced were found to possess good crystallinity (66%), β-phase presence (94%) and a microstructure based on spherulite agglomerations with a diameter of spherulite in the order of 1 μm that is higher as the polymer concentration increases. The membranes have good mechanical properties and the storage modulus, with values between 5 and 47 MPa, rises with the polymer content of the starting solution. Porcine bone marrow mesenchymal stem cells (pBM-MSCs) were used to study cell adhesion and proliferation. Regarding cell adhesion at 24 h, the cells preferred the more porous structures and had round focal adhesions with well-developed cytoskeletons, while they had a round morphology on the less porous membranes. The cells preferred the less porous membranes to proliferate, even though the initial morphology at 24 h showed poor adhesion. These findings confirm that the controlled microporosity of β-phase PVDF membranes can be produced by freeze extraction and offer the possibility of modifying the adhesion and proliferation of pBM-MSCs on these substrates. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/64783 |
url |
https://hdl.handle.net/1822/64783 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Morales-Román, R. M., Guillot-Ferriols, M., Roig-Pérez, L., Lanceros-Mendez, S., Gallego-Ferrer, G., & Gómez Ribelles, J. L. (2019, October). Freeze-extraction microporous electroactive supports for cell culture. European Polymer Journal. Elsevier BV. http://doi.org/10.1016/j.eurpolymj.2019.07.011 0014-3057 1873-1945 10.1016/j.eurpolymj.2019.07.011 https://www.sciencedirect.com/science/article/pii/S0014305719306913 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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