Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18714 |
Resumo: | The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7. |
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Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formationSkimmed milkReactive oxygen speciesNitric oxide formationThe capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7.ElsevierRepositório Científico do Instituto Politécnico do PortoPinho, Susana C. M.Faria, Miguel A.Melo, ArmindoPinto, EdgarAlmeida, AgostinhoAlves, RuiCabrita, Ana R. J.Fonseca, António J. M.Ferreira, Isabel M. P. V. O.2021-10-15T14:01:37Z2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18714engPinho, S. C. M., et al. (2021). "Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation." International Dairy Journal 118: 105046.0958-694610.1016/j.idairyj.2021.105046metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:11:27Zoai:recipp.ipp.pt:10400.22/18714Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:46.997580Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
title |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
spellingShingle |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation Pinho, Susana C. M. Skimmed milk Reactive oxygen species Nitric oxide formation |
title_short |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
title_full |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
title_fullStr |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
title_full_unstemmed |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
title_sort |
Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formation |
author |
Pinho, Susana C. M. |
author_facet |
Pinho, Susana C. M. Faria, Miguel A. Melo, Armindo Pinto, Edgar Almeida, Agostinho Alves, Rui Cabrita, Ana R. J. Fonseca, António J. M. Ferreira, Isabel M. P. V. O. |
author_role |
author |
author2 |
Faria, Miguel A. Melo, Armindo Pinto, Edgar Almeida, Agostinho Alves, Rui Cabrita, Ana R. J. Fonseca, António J. M. Ferreira, Isabel M. P. V. O. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Pinho, Susana C. M. Faria, Miguel A. Melo, Armindo Pinto, Edgar Almeida, Agostinho Alves, Rui Cabrita, Ana R. J. Fonseca, António J. M. Ferreira, Isabel M. P. V. O. |
dc.subject.por.fl_str_mv |
Skimmed milk Reactive oxygen species Nitric oxide formation |
topic |
Skimmed milk Reactive oxygen species Nitric oxide formation |
description |
The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-15T14:01:37Z 2021-07 2021-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18714 |
url |
http://hdl.handle.net/10400.22/18714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinho, S. C. M., et al. (2021). "Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation." International Dairy Journal 118: 105046. 0958-6946 10.1016/j.idairyj.2021.105046 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131475932610560 |