Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)

Detalhes bibliográficos
Autor(a) principal: Mall,Puspadhwaja
Data de Publicação: 2010
Outros Autores: Mohanty,Bijaya Kumar, Patankar,Dhananjay Bhawanishankar, Mody,Rustom, Tunga,Rashbehari
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025
Resumo: The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0)C while the optimal temperature for biomass production was 37(0)C.
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spelling Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)NisinLactococcus lactisStreptococcus agalactaeskimmed milkThe influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0)C while the optimal temperature for biomass production was 37(0)C.Instituto de Tecnologia do Paraná - Tecpar2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025Brazilian Archives of Biology and Technology v.53 n.1 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000100025info:eu-repo/semantics/openAccessMall,PuspadhwajaMohanty,Bijaya KumarPatankar,Dhananjay BhawanishankarMody,RustomTunga,Rashbeharieng2010-02-25T00:00:00Zoai:scielo:S1516-89132010000100025Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-02-25T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
title Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
spellingShingle Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
Mall,Puspadhwaja
Nisin
Lactococcus lactis
Streptococcus agalactae
skimmed milk
title_short Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
title_full Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
title_fullStr Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
title_full_unstemmed Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
title_sort Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440)
author Mall,Puspadhwaja
author_facet Mall,Puspadhwaja
Mohanty,Bijaya Kumar
Patankar,Dhananjay Bhawanishankar
Mody,Rustom
Tunga,Rashbehari
author_role author
author2 Mohanty,Bijaya Kumar
Patankar,Dhananjay Bhawanishankar
Mody,Rustom
Tunga,Rashbehari
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mall,Puspadhwaja
Mohanty,Bijaya Kumar
Patankar,Dhananjay Bhawanishankar
Mody,Rustom
Tunga,Rashbehari
dc.subject.por.fl_str_mv Nisin
Lactococcus lactis
Streptococcus agalactae
skimmed milk
topic Nisin
Lactococcus lactis
Streptococcus agalactae
skimmed milk
description The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth) was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v) with 0.15μg/ml of nisin supplemented with 0.5% (v/v) skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0)C while the optimal temperature for biomass production was 37(0)C.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000100025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.1 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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