L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.3390/molecules24173111 |
Resumo: | l-Buthionine sulfoximine (l-BSO) is an adjuvant drug that is reported to increase the sensitivity of cancer cells to neoplastic agents. Dendrimers are exceptional drug delivery systems and l-BSO nanoformulations are envisaged as potential chemotherapeutics. The absorption of l-BSO at a low wavelength limits its detection by conventional analytical tools. A simple and sensitive method for l-BSO detection and quantification is now reported. In this study, l-BSO was encapsulated in a folate-targeted generation four polyurea dendrimer (PUREG4-FA2) and its release profile was followed for 24 h at pH 7.4 and 37 ◦C. The protocol uses in situ l-BSO derivatization, by the formation of a catechol-derived orto-quinone, followed by visible detection of the derivative at 503 nm. The structure of the studied l-BSO derivative was assessed by NMR spectroscopy. |
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L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulationsButhionine sulfoximineCatecholDerivatizationNanoformulationPolyurea dendrimersUV-Vis detectionAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistrySDG 3 - Good Health and Well-beingl-Buthionine sulfoximine (l-BSO) is an adjuvant drug that is reported to increase the sensitivity of cancer cells to neoplastic agents. Dendrimers are exceptional drug delivery systems and l-BSO nanoformulations are envisaged as potential chemotherapeutics. The absorption of l-BSO at a low wavelength limits its detection by conventional analytical tools. A simple and sensitive method for l-BSO detection and quantification is now reported. In this study, l-BSO was encapsulated in a folate-targeted generation four polyurea dendrimer (PUREG4-FA2) and its release profile was followed for 24 h at pH 7.4 and 37 ◦C. The protocol uses in situ l-BSO derivatization, by the formation of a catechol-derived orto-quinone, followed by visible detection of the derivative at 503 nm. The structure of the studied l-BSO derivative was assessed by NMR spectroscopy.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMota, PedroPires, Rita F.Serpa, JacintaBonifácio, Vasco D.B.2019-09-10T22:49:26Z2019-08-272019-08-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/molecules24173111engPURE: 14632436http://www.scopus.com/inward/record.url?scp=85071401447&partnerID=8YFLogxKhttps://doi.org/10.3390/molecules24173111info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:44Zoai:run.unl.pt:10362/80766Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:55.604768Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
title |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
spellingShingle |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations Mota, Pedro Buthionine sulfoximine Catechol Derivatization Nanoformulation Polyurea dendrimers UV-Vis detection Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry SDG 3 - Good Health and Well-being |
title_short |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
title_full |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
title_fullStr |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
title_full_unstemmed |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
title_sort |
L-Buthionine sulfoximine detection and quantification in polyurea dendrimer nanoformulations |
author |
Mota, Pedro |
author_facet |
Mota, Pedro Pires, Rita F. Serpa, Jacinta Bonifácio, Vasco D.B. |
author_role |
author |
author2 |
Pires, Rita F. Serpa, Jacinta Bonifácio, Vasco D.B. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Mota, Pedro Pires, Rita F. Serpa, Jacinta Bonifácio, Vasco D.B. |
dc.subject.por.fl_str_mv |
Buthionine sulfoximine Catechol Derivatization Nanoformulation Polyurea dendrimers UV-Vis detection Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry SDG 3 - Good Health and Well-being |
topic |
Buthionine sulfoximine Catechol Derivatization Nanoformulation Polyurea dendrimers UV-Vis detection Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry SDG 3 - Good Health and Well-being |
description |
l-Buthionine sulfoximine (l-BSO) is an adjuvant drug that is reported to increase the sensitivity of cancer cells to neoplastic agents. Dendrimers are exceptional drug delivery systems and l-BSO nanoformulations are envisaged as potential chemotherapeutics. The absorption of l-BSO at a low wavelength limits its detection by conventional analytical tools. A simple and sensitive method for l-BSO detection and quantification is now reported. In this study, l-BSO was encapsulated in a folate-targeted generation four polyurea dendrimer (PUREG4-FA2) and its release profile was followed for 24 h at pH 7.4 and 37 ◦C. The protocol uses in situ l-BSO derivatization, by the formation of a catechol-derived orto-quinone, followed by visible detection of the derivative at 503 nm. The structure of the studied l-BSO derivative was assessed by NMR spectroscopy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-10T22:49:26Z 2019-08-27 2019-08-27T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.3390/molecules24173111 |
url |
https://doi.org/10.3390/molecules24173111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PURE: 14632436 http://www.scopus.com/inward/record.url?scp=85071401447&partnerID=8YFLogxK https://doi.org/10.3390/molecules24173111 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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