Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers

Detalhes bibliográficos
Autor(a) principal: Conde, Joao
Data de Publicação: 2020
Outros Autores: Bonifácio, Vasco D.B., Pires, Rita F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/154198
Resumo: Fundação para a Ciência e a Tecnologia for financial support through project PTDC/MEC-ONC/29327/2017 and the PhD grant SFRH/BD/109006/2015 (R.F.P.). We also thank Dr. Luisa Maia (UCIBIO, FCT-UNL) for EPR measurements.
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spelling Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimersGreen chemistryHuman mesenchymal stem cellsOsteogenic differentiationPolyurea dendrimersPolyurea oxide dendrimersBiomaterialsChemistry(all)Biomedical EngineeringBiochemistry, medicalSDG 3 - Good Health and Well-beingFundação para a Ciência e a Tecnologia for financial support through project PTDC/MEC-ONC/29327/2017 and the PhD grant SFRH/BD/109006/2015 (R.F.P.). We also thank Dr. Luisa Maia (UCIBIO, FCT-UNL) for EPR measurements.Polyurea oxide (PURO) biodendrimers are a class of dendrimers that can trigger osteogenic differentiation of human mesenchymal stem cells (hMSCs). PURO biodendrimers are prepared by simple, solventless oxidation of polyurea dendrimers using hydrogen peroxide as the oxidant in quantitative yield, retaining both biocompatibility (up to 10 mg/mL for higher generations) and the non-traditional intrinsic luminescence. The effect of PURO biodendrimers in the differentiation of hMSCs was found by the single addition to a standard growth medium for MSCs differentiation (without differentiation inducers). After 21 days of incubation, the formation of osteoblasts was confirmed by the alizarin red staining assay and alkaline phosphatase activity. This is the first report of in vitro osteodifferentiation fully regulated by synthetic soft polymers such as dendrimers. Current osteogenic differentiation protocols rely on an in vitro inducing formulation (including dexamethasone, ascorbic acid, and β-glycerophosphate), which lacks therapeutic potential in vivo. The outstanding role of dendrimers in nanomedicine, under clinic translation, combined with this feature is envisaged to foster PURO dendrimers as an important strategy in cell therapy and regenerative medicine.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNConde, JoaoBonifácio, Vasco D.B.Pires, Rita F.2023-06-20T22:18:29Z2020-12-212020-12-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/154198eng2576-6422PURE: 27073835https://doi.org/10.1021/acsabm.0c01315info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:36:38Zoai:run.unl.pt:10362/154198Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:31.579110Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
title Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
spellingShingle Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
Conde, Joao
Green chemistry
Human mesenchymal stem cells
Osteogenic differentiation
Polyurea dendrimers
Polyurea oxide dendrimers
Biomaterials
Chemistry(all)
Biomedical Engineering
Biochemistry, medical
SDG 3 - Good Health and Well-being
title_short Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
title_full Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
title_fullStr Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
title_full_unstemmed Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
title_sort Osteogenic differentiation of human mesenchymal stem cells by the single action of luminescent polyurea oxide biodendrimers
author Conde, Joao
author_facet Conde, Joao
Bonifácio, Vasco D.B.
Pires, Rita F.
author_role author
author2 Bonifácio, Vasco D.B.
Pires, Rita F.
author2_role author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centre for Toxicogenomics and Human Health (ToxOmics)
RUN
dc.contributor.author.fl_str_mv Conde, Joao
Bonifácio, Vasco D.B.
Pires, Rita F.
dc.subject.por.fl_str_mv Green chemistry
Human mesenchymal stem cells
Osteogenic differentiation
Polyurea dendrimers
Polyurea oxide dendrimers
Biomaterials
Chemistry(all)
Biomedical Engineering
Biochemistry, medical
SDG 3 - Good Health and Well-being
topic Green chemistry
Human mesenchymal stem cells
Osteogenic differentiation
Polyurea dendrimers
Polyurea oxide dendrimers
Biomaterials
Chemistry(all)
Biomedical Engineering
Biochemistry, medical
SDG 3 - Good Health and Well-being
description Fundação para a Ciência e a Tecnologia for financial support through project PTDC/MEC-ONC/29327/2017 and the PhD grant SFRH/BD/109006/2015 (R.F.P.). We also thank Dr. Luisa Maia (UCIBIO, FCT-UNL) for EPR measurements.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-21
2020-12-21T00:00:00Z
2023-06-20T22:18:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/154198
url http://hdl.handle.net/10362/154198
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2576-6422
PURE: 27073835
https://doi.org/10.1021/acsabm.0c01315
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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