Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients

Detalhes bibliográficos
Autor(a) principal: Soares, Rui S.
Data de Publicação: 2011
Outros Autores: Tendeiro, Rita, Foxall, Russell B., Baptista, António P., Cavaleiro, Rita, Gomes, Perpétua, Camacho, Ricardo, Valadas, Emília, Doroana, Manuela, Lucas, Margarida, Antunes, Francisco, Victorino, Rui M. M., Sousa, Ana E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/11304
Resumo: Copyright © 2011, American Society for Microbiology. All Rights Reserved.
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spelling Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patientsCopyright © 2011, American Society for Microbiology. All Rights Reserved.Viremia is significantly lower in HIV-2 than in HIV-1 infection, irrespective of disease stage. Nevertheless, the comparable proviral DNA burdens observed for these two infections indicate similar numbers of infected cells. Here we investigated this apparent paradox by assessing cell-associated viral replication. We found that untreated HIV-1-positive (HIV-1(+)) and HIV-2(+) individuals, matched for CD4 T cell depletion, exhibited similar gag mRNA levels, indicating that significant viral transcription is occurring in untreated HIV-2(+) patients, despite the reduced viremia (undetectable to 2.6 × 10(4) RNA copies/ml). However, tat mRNA transcripts were observed at significantly lower levels in HIV-2(+) patients, suggesting that the rate of de novo infection is decreased in these patients. Our data also reveal a direct relationship of gag and tat transcripts with CD4 and CD8 T cell activation, respectively. Antiretroviral therapy (ART)-treated HIV-2(+) patients showed persistent viral replication, irrespective of plasma viremia, possibly contributing to the emergence of drug resistance mutations, persistent hyperimmune activation, and poor CD4 T cell recovery that we observed with these individuals. In conclusion, we provide here evidence of significant ongoing viral replication in HIV-2(+) patients, further emphasizing the dichotomy between amount of plasma virus and cell-associated viral burden and stressing the need for antiretroviral trials and the definition of therapeutic guidelines for HIV-2 infection.This study was funded by grants from Fundação para a Ciência e a Tecnologia (FCT) and Programa Operacional Ciência e Inovação 2010 (POCI2010), as well as from Fundação Calouste Gulbenkian, to A.E.S. A.P.B. received a scholarship from GlaxoSmithKline, and R.S.S., R.T., R.B.F., and R.C. a scholarship from FCT.American Society for MicrobiologyRepositório da Universidade de LisboaSoares, Rui S.Tendeiro, RitaFoxall, Russell B.Baptista, António P.Cavaleiro, RitaGomes, PerpétuaCamacho, RicardoValadas, EmíliaDoroana, ManuelaLucas, MargaridaAntunes, FranciscoVictorino, Rui M. M.Sousa, Ana E.2014-06-19T15:13:36Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/11304engJournal of Virology Mar. 2011, p. 2429–24380022-538Xhttp://jvi.asm.org/content/85/5/2429info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:57:22Zoai:repositorio.ul.pt:10451/11304Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:35:00.183611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
title Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
spellingShingle Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
Soares, Rui S.
title_short Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
title_full Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
title_fullStr Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
title_full_unstemmed Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
title_sort Cell-associated viral burden provides evidence of ongoing viral replication in aviremic HIV-2-infected patients
author Soares, Rui S.
author_facet Soares, Rui S.
Tendeiro, Rita
Foxall, Russell B.
Baptista, António P.
Cavaleiro, Rita
Gomes, Perpétua
Camacho, Ricardo
Valadas, Emília
Doroana, Manuela
Lucas, Margarida
Antunes, Francisco
Victorino, Rui M. M.
Sousa, Ana E.
author_role author
author2 Tendeiro, Rita
Foxall, Russell B.
Baptista, António P.
Cavaleiro, Rita
Gomes, Perpétua
Camacho, Ricardo
Valadas, Emília
Doroana, Manuela
Lucas, Margarida
Antunes, Francisco
Victorino, Rui M. M.
Sousa, Ana E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Soares, Rui S.
Tendeiro, Rita
Foxall, Russell B.
Baptista, António P.
Cavaleiro, Rita
Gomes, Perpétua
Camacho, Ricardo
Valadas, Emília
Doroana, Manuela
Lucas, Margarida
Antunes, Francisco
Victorino, Rui M. M.
Sousa, Ana E.
description Copyright © 2011, American Society for Microbiology. All Rights Reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2014-06-19T15:13:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/11304
url http://hdl.handle.net/10451/11304
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Virology Mar. 2011, p. 2429–2438
0022-538X
http://jvi.asm.org/content/85/5/2429
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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