Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.

Detalhes bibliográficos
Autor(a) principal: Sá-Nogueira, Isabel de
Data de Publicação: 2006
Outros Autores: Franco, Irina Saraiva, Mota, Luís Jaime, Soares, Cláudio Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4205
Resumo: Journal of Bacteriology (Apr 2006) 3024-3036
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spelling Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.Journal of Bacteriology (Apr 2006) 3024-3036The Bacillus subtilis AraR transcription factor represses at least 13 genes required for the extracellular degradation of arabinose-containing polysaccharides, transport of arabinose, arabinose oligomers, xylose, and galactose, intracellular degradation of arabinose oligomers, and further catabolism of this sugar. AraR exhibits a chimeric organization comprising a small N-terminal DNA-binding domain that contains a winged helix-turn-helix motif similar to that seen with the GntR family and a larger C-terminal domain homologous to that of the LacI/GalR family. Here, a model for AraR was derived based on the known crystal structures of the FadR and PurR regulators from Escherichia coli. We have used random mutagenesis, deletion, and construction of chimeric LexA-AraR fusion proteins to map the functional domains of AraR required for DNA binding, dimerization, and effector binding. Moreover, predictions for the functional role of specific residues were tested by site-directed mutagenesis. In vivo analysis identified particular amino acids required for dimer assembly, formation of the nucleoprotein complex, and composition of the sugar-binding cleft. This work presents a structural framework for the function of AraR and provides insight into the mechanistic mode of action of this modular repressor.This work was partially supported by grants POCTI/BME/36164/00 and POCI/BIA-MIC/61140/04 from Fundacao para a Ciencia e Tecnologia (FCT) and FEDER to I.D.S.-N. I.S.F. is the holder of Ph.D. fellowship SFRH/BD/5233/01 from Fundacao para a Cieˆncia e Tecnologia (FCT).American Society for MicrobiologyRUNSá-Nogueira, Isabel deFranco, Irina SaraivaMota, Luís JaimeSoares, Cláudio Manuel2010-10-25T11:56:30Z2006-032006-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4205engFranco, S. I., Mota, L. J., Soares, C. M., and I. Sá-Nogueira. (2006). Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding. Journal of Bacteriology 188: 3024-3036.0021-9193 (Print)info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:33:53Zoai:run.unl.pt:10362/4205Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:35.806002Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
title Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
spellingShingle Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
Sá-Nogueira, Isabel de
title_short Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
title_full Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
title_fullStr Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
title_full_unstemmed Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
title_sort Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding.
author Sá-Nogueira, Isabel de
author_facet Sá-Nogueira, Isabel de
Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
author_role author
author2 Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sá-Nogueira, Isabel de
Franco, Irina Saraiva
Mota, Luís Jaime
Soares, Cláudio Manuel
description Journal of Bacteriology (Apr 2006) 3024-3036
publishDate 2006
dc.date.none.fl_str_mv 2006-03
2006-03-01T00:00:00Z
2010-10-25T11:56:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4205
url http://hdl.handle.net/10362/4205
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Franco, S. I., Mota, L. J., Soares, C. M., and I. Sá-Nogueira. (2006). Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding. Journal of Bacteriology 188: 3024-3036.
0021-9193 (Print)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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