Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/35371 |
Resumo: | Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses. |
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Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranespoly(l-lactic acid)tissue and biomedical engineeringcell-material interfaceCiências Médicas::Biotecnologia MédicaScience & TechnologyPoly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses.Portuguese Foundation for Science and Technology (FCT); contract grant numbers: C/FIS/UI607/2011, PTDC/CTM-NAN/112574/2009, contract grant numbers: SFRH/BPD/63148/2009, SFRH/BPD/90870/2012, Matepro—Optimizing Materials and Processes, Programa Operacional Regional do Norte, Quadro de Referência Estratégico Nacional” (QREN), “Fundo Europeu de Desenvolvimento Regional” (FEDER); contract grant number: NORTE-07–0124-FEDER-000037.John Wiley and SonsUniversidade do MinhoRibeiro, ClarisseSencadas, Vítor João Gomes SilvaAreias, Anabela C.Gama, F. M.Lanceros-Méndez, S.2015-072015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/35371engRibeiro, Clarisse; Sencadas, Vitor; Areias, Anabela C.; Gama, F. M.; Lanceros-Méndez, Senentxu, Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes. Journal of Biomedical Materials Research - Part A, 103(7), 2260-2268, 20151549-32961549-329610.1002/jbm.a.3536725370449http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35367/abstract;jsessionid=8A2C1C03714C3DDC3CA6D37F61DF5247.f01t03info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:11Zoai:repositorium.sdum.uminho.pt:1822/35371Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:08.916147Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
title |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
spellingShingle |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes Ribeiro, Clarisse poly(l-lactic acid) tissue and biomedical engineering cell-material interface Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
title_full |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
title_fullStr |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
title_full_unstemmed |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
title_sort |
Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes |
author |
Ribeiro, Clarisse |
author_facet |
Ribeiro, Clarisse Sencadas, Vítor João Gomes Silva Areias, Anabela C. Gama, F. M. Lanceros-Méndez, S. |
author_role |
author |
author2 |
Sencadas, Vítor João Gomes Silva Areias, Anabela C. Gama, F. M. Lanceros-Méndez, S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Clarisse Sencadas, Vítor João Gomes Silva Areias, Anabela C. Gama, F. M. Lanceros-Méndez, S. |
dc.subject.por.fl_str_mv |
poly(l-lactic acid) tissue and biomedical engineering cell-material interface Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
poly(l-lactic acid) tissue and biomedical engineering cell-material interface Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 2015-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/35371 |
url |
https://hdl.handle.net/1822/35371 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, Clarisse; Sencadas, Vitor; Areias, Anabela C.; Gama, F. M.; Lanceros-Méndez, Senentxu, Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes. Journal of Biomedical Materials Research - Part A, 103(7), 2260-2268, 2015 1549-3296 1549-3296 10.1002/jbm.a.35367 25370449 http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35367/abstract;jsessionid=8A2C1C03714C3DDC3CA6D37F61DF5247.f01t03 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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