Decrease of CD68 synovial macrophages in celastrol treated arthritic rats

Detalhes bibliográficos
Autor(a) principal: Cascao, Rita
Data de Publicação: 2015
Outros Autores: Vidal, Bruno, Lopes, Inês, Paisana, Eunice, Rino, José, Moita, Luis, Fonseca, João Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/53427
Resumo: Copyright: © 2015 Cascão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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spelling Decrease of CD68 synovial macrophages in celastrol treated arthritic ratsCopyright: © 2015 Cascão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedBackground: Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease characterized by cellular infiltration into the joints, hyperproliferation of synovial cells and bone damage. Available treatments for RA only induce remission in around 30% of the patients, have important adverse effects and its use is limited by their high cost. Therefore, compounds that can control arthritis, with an acceptable safety profile and low production costs are still an unmet need. We have shown, in vitro, that celastrol inhibits both IL-1β and TNF, which play an important role in RA, and, in vivo, that celastrol has significant anti-inflammatory properties. Our main goal in this work was to test the effect of celastrol in the number of sublining CD68 macrophages (a biomarker of therapeutic response for novel RA treatments) and on the overall synovial tissue cellularity and joint structure in the adjuvant-induced rat model of arthritis (AIA). Methods: Celastrol was administered to AIA rats both in the early (4 days after disease induction) and late (11 days after disease induction) phases of arthritis development. The inflammatory score, ankle perimeter and body weight were evaluated during treatment period. Rats were sacrificed after 22 days of disease progression and blood, internal organs and paw samples were collected for toxicological blood parameters and serum proinflammatory cytokine quantification, as well as histopathological and immunohistochemical evaluation, respectively. Results: Here we report that celastrol significantly decreases the number of sublining CD68 macrophages and the overall synovial inflammatory cellularity, and halted joint destruction without side effects. Conclusions: Our results validate celastrol as a promising compound for the treatment of arthritis.RC was supported with a fellowship from Fundação para a Ciência e a Tecnologia (FCT, SFRH/BPD/92860/2013).PLoSRepositório da Universidade de LisboaCascao, RitaVidal, BrunoLopes, InêsPaisana, EuniceRino, JoséMoita, LuisFonseca, João Eurico2022-06-21T15:31:38Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53427engPLoS One. 2015 Dec 11;10(12):e014244810.1371/journal.pone.01424481932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:59:11Zoai:repositorio.ul.pt:10451/53427Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:22.590986Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
title Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
spellingShingle Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
Cascao, Rita
title_short Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
title_full Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
title_fullStr Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
title_full_unstemmed Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
title_sort Decrease of CD68 synovial macrophages in celastrol treated arthritic rats
author Cascao, Rita
author_facet Cascao, Rita
Vidal, Bruno
Lopes, Inês
Paisana, Eunice
Rino, José
Moita, Luis
Fonseca, João Eurico
author_role author
author2 Vidal, Bruno
Lopes, Inês
Paisana, Eunice
Rino, José
Moita, Luis
Fonseca, João Eurico
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cascao, Rita
Vidal, Bruno
Lopes, Inês
Paisana, Eunice
Rino, José
Moita, Luis
Fonseca, João Eurico
description Copyright: © 2015 Cascão et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2022-06-21T15:31:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/53427
url http://hdl.handle.net/10451/53427
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS One. 2015 Dec 11;10(12):e0142448
10.1371/journal.pone.0142448
1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLoS
publisher.none.fl_str_mv PLoS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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