Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/2036 |
Resumo: | We characterized 35 single nucleotide polymorphism (SNP) markers for the brown alga Fucus vesiculosus. Based on existing Fucus Expressed Sequence Tag libraries for heat and desiccation-stressed tissue, SNPs were developed and confirmed by re-sequencing cDNA from a diverse panel of individuals. SNP loci were genotyped using the SEQUENOM single base extension iPLEXTM system for multiplex assays on the MassARRAY platform, which uses matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) to discriminate allele-specific products. The SNP markers showed a wide range of variability among 16 populations from the south-west of the UK, northern Portugal and Morocco. The analysis of the information provided by these markers will be useful for studying population structure, historical demography and phylogeography of F. vesiculosus. They can also be used for the identification of genes and/or linked genomic regions potentially subject to selection in response to abiotic stressors like temperature extremes and desiccation intensity that vary across habitats and geographical range. |
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Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosusexpressed sequence tagfucoid algaeFucus vesiculosusMassARRAYselectionPhaeophyceaephylogeographysingle nucleotide polymorphismSNP genetic markerWe characterized 35 single nucleotide polymorphism (SNP) markers for the brown alga Fucus vesiculosus. Based on existing Fucus Expressed Sequence Tag libraries for heat and desiccation-stressed tissue, SNPs were developed and confirmed by re-sequencing cDNA from a diverse panel of individuals. SNP loci were genotyped using the SEQUENOM single base extension iPLEXTM system for multiplex assays on the MassARRAY platform, which uses matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) to discriminate allele-specific products. The SNP markers showed a wide range of variability among 16 populations from the south-west of the UK, northern Portugal and Morocco. The analysis of the information provided by these markers will be useful for studying population structure, historical demography and phylogeography of F. vesiculosus. They can also be used for the identification of genes and/or linked genomic regions potentially subject to selection in response to abiotic stressors like temperature extremes and desiccation intensity that vary across habitats and geographical range.Fundação para a Ciência e TecnologiaTaylor & Francis GroupSapientiaCánovas, Fernando G.Mota, CatarinaFerreira Costa, J.Serrão, EsterCoyer, J. A.Olsen, J. L.Pearson, G. A.2012-12-19T12:04:37Z2011-10-182011-10-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/2036eng1469-4433AUT: ESE00527;info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:13:05Zoai:sapientia.ualg.pt:10400.1/2036Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:56:00.696930Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
title |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
spellingShingle |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus Cánovas, Fernando G. expressed sequence tag fucoid algae Fucus vesiculosus MassARRAY selection Phaeophyceae phylogeography single nucleotide polymorphism SNP genetic marker |
title_short |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
title_full |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
title_fullStr |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
title_full_unstemmed |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
title_sort |
Development and characterization of 35 single nucleotide polymorphism markers for the brown alga Fucus vesiculosus |
author |
Cánovas, Fernando G. |
author_facet |
Cánovas, Fernando G. Mota, Catarina Ferreira Costa, J. Serrão, Ester Coyer, J. A. Olsen, J. L. Pearson, G. A. |
author_role |
author |
author2 |
Mota, Catarina Ferreira Costa, J. Serrão, Ester Coyer, J. A. Olsen, J. L. Pearson, G. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Cánovas, Fernando G. Mota, Catarina Ferreira Costa, J. Serrão, Ester Coyer, J. A. Olsen, J. L. Pearson, G. A. |
dc.subject.por.fl_str_mv |
expressed sequence tag fucoid algae Fucus vesiculosus MassARRAY selection Phaeophyceae phylogeography single nucleotide polymorphism SNP genetic marker |
topic |
expressed sequence tag fucoid algae Fucus vesiculosus MassARRAY selection Phaeophyceae phylogeography single nucleotide polymorphism SNP genetic marker |
description |
We characterized 35 single nucleotide polymorphism (SNP) markers for the brown alga Fucus vesiculosus. Based on existing Fucus Expressed Sequence Tag libraries for heat and desiccation-stressed tissue, SNPs were developed and confirmed by re-sequencing cDNA from a diverse panel of individuals. SNP loci were genotyped using the SEQUENOM single base extension iPLEXTM system for multiplex assays on the MassARRAY platform, which uses matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) to discriminate allele-specific products. The SNP markers showed a wide range of variability among 16 populations from the south-west of the UK, northern Portugal and Morocco. The analysis of the information provided by these markers will be useful for studying population structure, historical demography and phylogeography of F. vesiculosus. They can also be used for the identification of genes and/or linked genomic regions potentially subject to selection in response to abiotic stressors like temperature extremes and desiccation intensity that vary across habitats and geographical range. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-18 2011-10-18T00:00:00Z 2012-12-19T12:04:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/2036 |
url |
http://hdl.handle.net/10400.1/2036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1469-4433 AUT: ESE00527; |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Group |
publisher.none.fl_str_mv |
Taylor & Francis Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133164829933568 |