Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis.
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.3/155 |
Resumo: | A vertebrae sample of tope shark, Galeorhinus galeus (Linnaeus, 1758), stratified by sizeclasses,was used to test between different staining techniques of enhancing vertebral growth ring visibility. Four techniques were tested: alizarin red, silver nitrate and cobalt nitrate staining, and the “deep-coned vertebrae” technique. The latter was discarded due to its unsuitability for tope shark vertebrae. The cobalt nitrate original protocol was modified. Upon staining, each vertebra was subject to three replicated independent readings, by a single reader. Within-reader consistency and bias of growth ring counts determination were evaluated using four different precision indices: percent of agreement (PA), average percent error (APE), coefficient of variation (V) and index of precision (D). Results indicated that the vertebrae stained with the cobalt nitrate technique showed both better optic enhancement of growth rings and higher degree of count consistency, comparatively to the alizarin red and silver stained vertebrae. Conclusively, the cobalt nitrate was the most efficient staining technique upon tope shark vertebrae, among the methods tested herein. |
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Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis.CaçãoTope SharkGaleorhinus galeusAçoresAzoresA vertebrae sample of tope shark, Galeorhinus galeus (Linnaeus, 1758), stratified by sizeclasses,was used to test between different staining techniques of enhancing vertebral growth ring visibility. Four techniques were tested: alizarin red, silver nitrate and cobalt nitrate staining, and the “deep-coned vertebrae” technique. The latter was discarded due to its unsuitability for tope shark vertebrae. The cobalt nitrate original protocol was modified. Upon staining, each vertebra was subject to three replicated independent readings, by a single reader. Within-reader consistency and bias of growth ring counts determination were evaluated using four different precision indices: percent of agreement (PA), average percent error (APE), coefficient of variation (V) and index of precision (D). Results indicated that the vertebrae stained with the cobalt nitrate technique showed both better optic enhancement of growth rings and higher degree of count consistency, comparatively to the alizarin red and silver stained vertebrae. Conclusively, the cobalt nitrate was the most efficient staining technique upon tope shark vertebrae, among the methods tested herein.Universidade dos AçoresRepositório da Universidade dos AçoresDuarte, Pedro N.Silva, Alexandre A.Menezes, Gui M.Pinho, Mário Rui2008-12-15T10:36:21Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/155eng"ARQUIPÉLAGO. Ciências Biológicas e Marinhas". ISSN 0873-4704. Nº 18A (2001): 65-740873-4704info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:27:18Zoai:repositorio.uac.pt:10400.3/155Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:23:11.037372Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
title |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
spellingShingle |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. Duarte, Pedro N. Cação Tope Shark Galeorhinus galeus Açores Azores |
title_short |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
title_full |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
title_fullStr |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
title_full_unstemmed |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
title_sort |
Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis. |
author |
Duarte, Pedro N. |
author_facet |
Duarte, Pedro N. Silva, Alexandre A. Menezes, Gui M. Pinho, Mário Rui |
author_role |
author |
author2 |
Silva, Alexandre A. Menezes, Gui M. Pinho, Mário Rui |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade dos Açores |
dc.contributor.author.fl_str_mv |
Duarte, Pedro N. Silva, Alexandre A. Menezes, Gui M. Pinho, Mário Rui |
dc.subject.por.fl_str_mv |
Cação Tope Shark Galeorhinus galeus Açores Azores |
topic |
Cação Tope Shark Galeorhinus galeus Açores Azores |
description |
A vertebrae sample of tope shark, Galeorhinus galeus (Linnaeus, 1758), stratified by sizeclasses,was used to test between different staining techniques of enhancing vertebral growth ring visibility. Four techniques were tested: alizarin red, silver nitrate and cobalt nitrate staining, and the “deep-coned vertebrae” technique. The latter was discarded due to its unsuitability for tope shark vertebrae. The cobalt nitrate original protocol was modified. Upon staining, each vertebra was subject to three replicated independent readings, by a single reader. Within-reader consistency and bias of growth ring counts determination were evaluated using four different precision indices: percent of agreement (PA), average percent error (APE), coefficient of variation (V) and index of precision (D). Results indicated that the vertebrae stained with the cobalt nitrate technique showed both better optic enhancement of growth rings and higher degree of count consistency, comparatively to the alizarin red and silver stained vertebrae. Conclusively, the cobalt nitrate was the most efficient staining technique upon tope shark vertebrae, among the methods tested herein. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 2001-01-01T00:00:00Z 2008-12-15T10:36:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.3/155 |
url |
http://hdl.handle.net/10400.3/155 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"ARQUIPÉLAGO. Ciências Biológicas e Marinhas". ISSN 0873-4704. Nº 18A (2001): 65-74 0873-4704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade dos Açores |
publisher.none.fl_str_mv |
Universidade dos Açores |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130676904067072 |