GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20193 |
Resumo: | Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration. |
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GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachmentScience & TechnologyMultilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration.EU projects Lidwine (contract no. FP6-026741)Ministerio de Ciencia e Innovaci on de España - (MICINN) - bolsa BES-2008-00374Royal Society of ChemistryUniversidade do MinhoFrancesko, AntonioCosta, Diana Soares daLisboa, P.Reis, R. L.Pashkuleva, I.Tzanov, Tzanko2012-062012-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20193eng0959-942810.1039/c2jm31051ahttp://pubs.rsc.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:58ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
title |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
spellingShingle |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment Francesko, Antonio Science & Technology |
title_short |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
title_full |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
title_fullStr |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
title_full_unstemmed |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
title_sort |
GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment |
author |
Francesko, Antonio |
author_facet |
Francesko, Antonio Costa, Diana Soares da Lisboa, P. Reis, R. L. Pashkuleva, I. Tzanov, Tzanko |
author_role |
author |
author2 |
Costa, Diana Soares da Lisboa, P. Reis, R. L. Pashkuleva, I. Tzanov, Tzanko |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Francesko, Antonio Costa, Diana Soares da Lisboa, P. Reis, R. L. Pashkuleva, I. Tzanov, Tzanko |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06 2012-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20193 |
url |
http://hdl.handle.net/1822/20193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0959-9428 10.1039/c2jm31051a http://pubs.rsc.org/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303688353677312 |