Polyhydroxyalkanoates from A Mixed Microbial Culture

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Ana Marta
Data de Publicação: 2022
Outros Autores: Franca, Rita Dias Guardão, Dionísio, Madalena, Sevrin, Chantal, Grandfils, Christian, Reis, Maria A. M., Lourenço, Nídia Dana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143926
Resumo: Funding: This work was funded by the INGREEN project that has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 838120. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium. This work was financed by national funds from FCT—Fundação para a Ciência e a Tecno-logia, I.P., in the scope of the project and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. A.M. Rodrigues acknowledges the financial support of FCT through the PhD grant 2020.08574.BD. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Polyhydroxyalkanoates from A Mixed Microbial CultureExtraction Optimization and Polymer Characterizationchemical digestiondesign of experimentsPHA extractionpolymer propertiesChemistry(all)Polymers and PlasticsFunding: This work was funded by the INGREEN project that has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 838120. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium. This work was financed by national funds from FCT—Fundação para a Ciência e a Tecno-logia, I.P., in the scope of the project and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. A.M. Rodrigues acknowledges the financial support of FCT through the PhD grant 2020.08574.BD. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Polyhydroxyalkanoates (PHA) are biopolymers with potential to replace conventional oil-based plastics. However, PHA high production costs limit their scope of commercial applications. Downstream processing is currently the major cost factor for PHA production but one of the least investigated aspects of the PHA production chain. In this study, the extraction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced at pilot scale by a mixed microbial culture was performed using sodium hydroxide (NaOH) or sodium hypochlorite (NaClO) as digestion agents of non-PHA cellular mass. Optimal conditions for digestion with NaOH (0.3 M, 4.8 h) and NaClO (9.0%, 3.4 h) resulted in polymers with a PHA purity and recovery of ca. 100%, in the case of the former and ca. 99% and 90%, respectively, in the case of the latter. These methods presented higher PHA recoveries than extraction by soxhlet with chloroform, the benchmark protocol for PHA extraction. The polymers extracted by the three methods presented similar PHA purities, molecular weights and polydispersity indices. Using the optimized conditions for NaOH and NaClO digestions, this study analyzed the effect of the initial intracellular PHA content (40–70%), biomass concentration (20–100 g/L) and biomass pre-treatment (fresh vs. dried vs. lyophilized) on the performance of PHA extraction by these two methods.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaLAQV@REQUIMTERUNRodrigues, Ana MartaFranca, Rita Dias GuardãoDionísio, MadalenaSevrin, ChantalGrandfils, ChristianReis, Maria A. M.Lourenço, Nídia Dana2022-09-21T22:37:53Z2022-05-252022-05-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22application/pdfhttp://hdl.handle.net/10362/143926engPURE: 46647992https://doi.org/10.3390/polym14112155info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:54Zoai:run.unl.pt:10362/143926Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:13.309370Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polyhydroxyalkanoates from A Mixed Microbial Culture
Extraction Optimization and Polymer Characterization
title Polyhydroxyalkanoates from A Mixed Microbial Culture
spellingShingle Polyhydroxyalkanoates from A Mixed Microbial Culture
Rodrigues, Ana Marta
chemical digestion
design of experiments
PHA extraction
polymer properties
Chemistry(all)
Polymers and Plastics
title_short Polyhydroxyalkanoates from A Mixed Microbial Culture
title_full Polyhydroxyalkanoates from A Mixed Microbial Culture
title_fullStr Polyhydroxyalkanoates from A Mixed Microbial Culture
title_full_unstemmed Polyhydroxyalkanoates from A Mixed Microbial Culture
title_sort Polyhydroxyalkanoates from A Mixed Microbial Culture
author Rodrigues, Ana Marta
author_facet Rodrigues, Ana Marta
Franca, Rita Dias Guardão
Dionísio, Madalena
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Lourenço, Nídia Dana
author_role author
author2 Franca, Rita Dias Guardão
Dionísio, Madalena
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Lourenço, Nídia Dana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Rodrigues, Ana Marta
Franca, Rita Dias Guardão
Dionísio, Madalena
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Lourenço, Nídia Dana
dc.subject.por.fl_str_mv chemical digestion
design of experiments
PHA extraction
polymer properties
Chemistry(all)
Polymers and Plastics
topic chemical digestion
design of experiments
PHA extraction
polymer properties
Chemistry(all)
Polymers and Plastics
description Funding: This work was funded by the INGREEN project that has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 838120. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium. This work was financed by national funds from FCT—Fundação para a Ciência e a Tecno-logia, I.P., in the scope of the project and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. A.M. Rodrigues acknowledges the financial support of FCT through the PhD grant 2020.08574.BD. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-21T22:37:53Z
2022-05-25
2022-05-25T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143926
url http://hdl.handle.net/10362/143926
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 46647992
https://doi.org/10.3390/polym14112155
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 22
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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