Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/4838 https://doi.org/10.1016/S0379-0738(00)00153-5 |
Resumo: | This paper describes an analytical method for strychnine determination in biological samples by gas chromatography/mass spectrometry and their application in the investigation of two cases involving strychnine ingestion: A fatal case and a clinical one. The strychnine is isolated from biological samples using a liquid-liquid extraction procedure. The clean-up procedure is performed using an acid solution. Papaverine is used as internal standard in the quantification of strychnine. In the analysed specimens, the limits of quantification were 0.1 [mu]g/ml or 0.1 [mu]g/g. The recovery rate ranged from 75.0% to 98.7% and the coefficients of variation ranged from 4.8% to 10.5%. |
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Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reportsStrychnineRodenticideAlkaloidThis paper describes an analytical method for strychnine determination in biological samples by gas chromatography/mass spectrometry and their application in the investigation of two cases involving strychnine ingestion: A fatal case and a clinical one. The strychnine is isolated from biological samples using a liquid-liquid extraction procedure. The clean-up procedure is performed using an acid solution. Papaverine is used as internal standard in the quantification of strychnine. In the analysed specimens, the limits of quantification were 0.1 [mu]g/ml or 0.1 [mu]g/g. The recovery rate ranged from 75.0% to 98.7% and the coefficients of variation ranged from 4.8% to 10.5%.http://www.sciencedirect.com/science/article/B6T6W-408KCFB-8/1/f8bbeefad9e20909aef50467f67a2e842000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/4838http://hdl.handle.net/10316/4838https://doi.org/10.1016/S0379-0738(00)00153-5engForensic Science International. 110:2 (2000) 145-152Marques, E. P.Gil, F.Proença, P.Monsanto, P.Oliveira, M. F.Castanheira, A.Vieira, D. N.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T01:42:47ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
title |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
spellingShingle |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports Marques, E. P. Strychnine Rodenticide Alkaloid |
title_short |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
title_full |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
title_fullStr |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
title_full_unstemmed |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
title_sort |
Analytical method for the determination of strychnine in tissues by gas chromatography/mass spectrometry: two case reports |
author |
Marques, E. P. |
author_facet |
Marques, E. P. Gil, F. Proença, P. Monsanto, P. Oliveira, M. F. Castanheira, A. Vieira, D. N. |
author_role |
author |
author2 |
Gil, F. Proença, P. Monsanto, P. Oliveira, M. F. Castanheira, A. Vieira, D. N. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, E. P. Gil, F. Proença, P. Monsanto, P. Oliveira, M. F. Castanheira, A. Vieira, D. N. |
dc.subject.por.fl_str_mv |
Strychnine Rodenticide Alkaloid |
topic |
Strychnine Rodenticide Alkaloid |
description |
This paper describes an analytical method for strychnine determination in biological samples by gas chromatography/mass spectrometry and their application in the investigation of two cases involving strychnine ingestion: A fatal case and a clinical one. The strychnine is isolated from biological samples using a liquid-liquid extraction procedure. The clean-up procedure is performed using an acid solution. Papaverine is used as internal standard in the quantification of strychnine. In the analysed specimens, the limits of quantification were 0.1 [mu]g/ml or 0.1 [mu]g/g. The recovery rate ranged from 75.0% to 98.7% and the coefficients of variation ranged from 4.8% to 10.5%. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/4838 http://hdl.handle.net/10316/4838 https://doi.org/10.1016/S0379-0738(00)00153-5 |
url |
http://hdl.handle.net/10316/4838 https://doi.org/10.1016/S0379-0738(00)00153-5 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Forensic Science International. 110:2 (2000) 145-152 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777302588680568832 |