Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.4/2190 |
Resumo: | AIM: To analyze the modulation of gene expression profile associated with inhibition of liver regeneration in hepatitis B X (HBx)-expressing transgenic mice. METHODS: Microarray technology was performed on liver tissue obtained from 4 control (LacZ) and 4 transgenic mice (HBx-LacZ), 48 h after partial hepatectomy. The significance of the normalized log-ratios was assessed for each gene, using robust t-tests under an empirical Bayes approach. Microarray hybridization data was verified on selected genes by quantitative PCR. RESULTS: The comparison of gene expression patterns showed a consistent modulation of the expression of 26 genes, most of which are implicated in liver regeneration. Up-regulated genes included DNA repair proteins (Rad-52, MSH6) and transmembrane proteins (syndecan 4, tetraspanin), while down-regulated genes were connected to the regulation of transcription (histone deacetylase, Zfp90, MyoD1) and were involved in the cholesterol metabolic pathway and isoprenoid biosynthesis (farnesyl diphosphate synthase, Cyp7b1, geranylgeranyl diphosphate synthase, SAA3). CONCLUSION: Our results provide a novel insight into the biological activities of HBx, implicated in the inhibition of liver regeneration. |
id |
RCAP_f875dd87a9a8b510817ea27d55e5ca7f |
---|---|
oai_identifier_str |
oai:rihuc.huc.min-saude.pt:10400.4/2190 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic miceHepatite B CrónicaRegeneração HepáticaAnálise de Sequência com Séries de OligonucleotídeosTransativadoresAIM: To analyze the modulation of gene expression profile associated with inhibition of liver regeneration in hepatitis B X (HBx)-expressing transgenic mice. METHODS: Microarray technology was performed on liver tissue obtained from 4 control (LacZ) and 4 transgenic mice (HBx-LacZ), 48 h after partial hepatectomy. The significance of the normalized log-ratios was assessed for each gene, using robust t-tests under an empirical Bayes approach. Microarray hybridization data was verified on selected genes by quantitative PCR. RESULTS: The comparison of gene expression patterns showed a consistent modulation of the expression of 26 genes, most of which are implicated in liver regeneration. Up-regulated genes included DNA repair proteins (Rad-52, MSH6) and transmembrane proteins (syndecan 4, tetraspanin), while down-regulated genes were connected to the regulation of transcription (histone deacetylase, Zfp90, MyoD1) and were involved in the cholesterol metabolic pathway and isoprenoid biosynthesis (farnesyl diphosphate synthase, Cyp7b1, geranylgeranyl diphosphate synthase, SAA3). CONCLUSION: Our results provide a novel insight into the biological activities of HBx, implicated in the inhibition of liver regeneration.RIHUCSidorkiewicz, MJais, JPTralhao, JGMorosan, SGiannini, CBrezillon, NSoussan, PDelpuech, OKremsdorf, D2018-11-28T15:45:43Z2008-01-282008-01-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/2190engWorld J Gastroenterol. 2008 Jan 28;14(4):574-81.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:23:31Zoai:rihuc.huc.min-saude.pt:10400.4/2190Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:04:39.421821Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
title |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
spellingShingle |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice Sidorkiewicz, M Hepatite B Crónica Regeneração Hepática Análise de Sequência com Séries de Oligonucleotídeos Transativadores |
title_short |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
title_full |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
title_fullStr |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
title_full_unstemmed |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
title_sort |
Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice |
author |
Sidorkiewicz, M |
author_facet |
Sidorkiewicz, M Jais, JP Tralhao, JG Morosan, S Giannini, C Brezillon, N Soussan, P Delpuech, O Kremsdorf, D |
author_role |
author |
author2 |
Jais, JP Tralhao, JG Morosan, S Giannini, C Brezillon, N Soussan, P Delpuech, O Kremsdorf, D |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
RIHUC |
dc.contributor.author.fl_str_mv |
Sidorkiewicz, M Jais, JP Tralhao, JG Morosan, S Giannini, C Brezillon, N Soussan, P Delpuech, O Kremsdorf, D |
dc.subject.por.fl_str_mv |
Hepatite B Crónica Regeneração Hepática Análise de Sequência com Séries de Oligonucleotídeos Transativadores |
topic |
Hepatite B Crónica Regeneração Hepática Análise de Sequência com Séries de Oligonucleotídeos Transativadores |
description |
AIM: To analyze the modulation of gene expression profile associated with inhibition of liver regeneration in hepatitis B X (HBx)-expressing transgenic mice. METHODS: Microarray technology was performed on liver tissue obtained from 4 control (LacZ) and 4 transgenic mice (HBx-LacZ), 48 h after partial hepatectomy. The significance of the normalized log-ratios was assessed for each gene, using robust t-tests under an empirical Bayes approach. Microarray hybridization data was verified on selected genes by quantitative PCR. RESULTS: The comparison of gene expression patterns showed a consistent modulation of the expression of 26 genes, most of which are implicated in liver regeneration. Up-regulated genes included DNA repair proteins (Rad-52, MSH6) and transmembrane proteins (syndecan 4, tetraspanin), while down-regulated genes were connected to the regulation of transcription (histone deacetylase, Zfp90, MyoD1) and were involved in the cholesterol metabolic pathway and isoprenoid biosynthesis (farnesyl diphosphate synthase, Cyp7b1, geranylgeranyl diphosphate synthase, SAA3). CONCLUSION: Our results provide a novel insight into the biological activities of HBx, implicated in the inhibition of liver regeneration. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-28 2008-01-28T00:00:00Z 2018-11-28T15:45:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.4/2190 |
url |
http://hdl.handle.net/10400.4/2190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World J Gastroenterol. 2008 Jan 28;14(4):574-81. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131708855943168 |