Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/3063 |
Resumo: | Introduction - Obesity became a major public health problem as a result of its increasing prevalence worldwide. Paraoxonase-1 (PON1) is an esterase able to protect membranes and lipoproteins from oxidative modifications. At the PON1 gene, several polymorphisms in the promoter and coding regions have been identified. The aims of this study were i) to assess PON1 L55M and Q192R polymorphisms as a risk factor for obesity in women; ii) to compare PON1 activity according to the expression of each allele in L55M and Q192R polymorphisms; iii) to compare PON1 activity between obese and normal-weight women. Materials and methods - We studied 75 healthy (35.9±8.2 years) and 81 obese women (34.3±8.2 years). Inclusion criteria for obese subjects were body mass index ≥30 kg/m2 and absence of inflammatory/neoplasic conditions or kidney/hepatic dysfunction. The two PON1 polymorphisms were assessed by real-time PCR with TaqMan probes. PON1 enzymatic activity was assessed by spectrophotometric methods, using paraoxon as a substrate. Results - No significant differences were found for PON1 activity between normal and obese women. Nevertheless, PON1 activity was greater (P<0.01) for the RR genotype (in Q192R polymorphism) and for the LL genotype (in L55M polymorphism). The frequency of allele R of Q192R polymorphism was significantly higher in obese women (P<0.05) and was associated with an increased risk of obesity (odds ratio=2.0 – 95% confidence interval (1.04; 3.87)). Conclusion - 55M and Q192R polymorphisms influence PON1 activity. The allele R of the Q192R polymorphism is associated with an increased risk for development of obesity among Portuguese Caucasian premenopausal women. |
id |
RCAP_f8f133a6164250287955e7872d8dff4c |
---|---|
oai_identifier_str |
oai:repositorio.ipl.pt:10400.21/3063 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese womenAllelesAnalysis of varianceAryldialkylphosphataseEuropean Continental Ancestry GroupGene frequencyGenetic association studiesGenetic predisposition to diseaseObesityOdds ratioPolymorphism, Single nucleotideReverse transcriptase polymerase chain reactionRisk factorPortugalIntroduction - Obesity became a major public health problem as a result of its increasing prevalence worldwide. Paraoxonase-1 (PON1) is an esterase able to protect membranes and lipoproteins from oxidative modifications. At the PON1 gene, several polymorphisms in the promoter and coding regions have been identified. The aims of this study were i) to assess PON1 L55M and Q192R polymorphisms as a risk factor for obesity in women; ii) to compare PON1 activity according to the expression of each allele in L55M and Q192R polymorphisms; iii) to compare PON1 activity between obese and normal-weight women. Materials and methods - We studied 75 healthy (35.9±8.2 years) and 81 obese women (34.3±8.2 years). Inclusion criteria for obese subjects were body mass index ≥30 kg/m2 and absence of inflammatory/neoplasic conditions or kidney/hepatic dysfunction. The two PON1 polymorphisms were assessed by real-time PCR with TaqMan probes. PON1 enzymatic activity was assessed by spectrophotometric methods, using paraoxon as a substrate. Results - No significant differences were found for PON1 activity between normal and obese women. Nevertheless, PON1 activity was greater (P<0.01) for the RR genotype (in Q192R polymorphism) and for the LL genotype (in L55M polymorphism). The frequency of allele R of Q192R polymorphism was significantly higher in obese women (P<0.05) and was associated with an increased risk of obesity (odds ratio=2.0 – 95% confidence interval (1.04; 3.87)). Conclusion - 55M and Q192R polymorphisms influence PON1 activity. The allele R of the Q192R polymorphism is associated with an increased risk for development of obesity among Portuguese Caucasian premenopausal women.European Society of EndocrinologyRCIPLVeiga, LuísaSilva-Nunes, JoséMelão, AliceOliveira, AnaDuarte, LeoneBrito, Miguel2014-01-03T21:02:01Z2011-022011-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/3063engVeiga L, Silva-Nunes J, Melão A, Oliveira A, Duarte L, Brito M. Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women. Eur J Endocrinol. 2011;164(2):213-8.1479-683Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:43:12Zoai:repositorio.ipl.pt:10400.21/3063Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:12:43.988040Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
title |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
spellingShingle |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women Veiga, Luísa Alleles Analysis of variance Aryldialkylphosphatase European Continental Ancestry Group Gene frequency Genetic association studies Genetic predisposition to disease Obesity Odds ratio Polymorphism, Single nucleotide Reverse transcriptase polymerase chain reaction Risk factor Portugal |
title_short |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
title_full |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
title_fullStr |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
title_full_unstemmed |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
title_sort |
Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women |
author |
Veiga, Luísa |
author_facet |
Veiga, Luísa Silva-Nunes, José Melão, Alice Oliveira, Ana Duarte, Leone Brito, Miguel |
author_role |
author |
author2 |
Silva-Nunes, José Melão, Alice Oliveira, Ana Duarte, Leone Brito, Miguel |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Veiga, Luísa Silva-Nunes, José Melão, Alice Oliveira, Ana Duarte, Leone Brito, Miguel |
dc.subject.por.fl_str_mv |
Alleles Analysis of variance Aryldialkylphosphatase European Continental Ancestry Group Gene frequency Genetic association studies Genetic predisposition to disease Obesity Odds ratio Polymorphism, Single nucleotide Reverse transcriptase polymerase chain reaction Risk factor Portugal |
topic |
Alleles Analysis of variance Aryldialkylphosphatase European Continental Ancestry Group Gene frequency Genetic association studies Genetic predisposition to disease Obesity Odds ratio Polymorphism, Single nucleotide Reverse transcriptase polymerase chain reaction Risk factor Portugal |
description |
Introduction - Obesity became a major public health problem as a result of its increasing prevalence worldwide. Paraoxonase-1 (PON1) is an esterase able to protect membranes and lipoproteins from oxidative modifications. At the PON1 gene, several polymorphisms in the promoter and coding regions have been identified. The aims of this study were i) to assess PON1 L55M and Q192R polymorphisms as a risk factor for obesity in women; ii) to compare PON1 activity according to the expression of each allele in L55M and Q192R polymorphisms; iii) to compare PON1 activity between obese and normal-weight women. Materials and methods - We studied 75 healthy (35.9±8.2 years) and 81 obese women (34.3±8.2 years). Inclusion criteria for obese subjects were body mass index ≥30 kg/m2 and absence of inflammatory/neoplasic conditions or kidney/hepatic dysfunction. The two PON1 polymorphisms were assessed by real-time PCR with TaqMan probes. PON1 enzymatic activity was assessed by spectrophotometric methods, using paraoxon as a substrate. Results - No significant differences were found for PON1 activity between normal and obese women. Nevertheless, PON1 activity was greater (P<0.01) for the RR genotype (in Q192R polymorphism) and for the LL genotype (in L55M polymorphism). The frequency of allele R of Q192R polymorphism was significantly higher in obese women (P<0.05) and was associated with an increased risk of obesity (odds ratio=2.0 – 95% confidence interval (1.04; 3.87)). Conclusion - 55M and Q192R polymorphisms influence PON1 activity. The allele R of the Q192R polymorphism is associated with an increased risk for development of obesity among Portuguese Caucasian premenopausal women. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02 2011-02-01T00:00:00Z 2014-01-03T21:02:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/3063 |
url |
http://hdl.handle.net/10400.21/3063 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veiga L, Silva-Nunes J, Melão A, Oliveira A, Duarte L, Brito M. Q192R polymorphism of the paraoxonase-1 gene as a risk factor for obesity in Portuguese women. Eur J Endocrinol. 2011;164(2):213-8. 1479-683X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
European Society of Endocrinology |
publisher.none.fl_str_mv |
European Society of Endocrinology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133382846709760 |