Polyurea dendrimer for efficient cytosolic siRNA delivery

Detalhes bibliográficos
Autor(a) principal: Restani, Rita
Data de Publicação: 2014
Outros Autores: Conde, João, Baptista, Pedro, Cidade, Maria Teresa Varanda, Bragança, Ana, Morgado, Jorge, Correia, Ilídio Joaquim Sobreira, Ricardo, Ana Aguiar, Bonifácio, Vasco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4663
Resumo: The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.
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spelling Polyurea dendrimer for efficient cytosolic siRNA deliverySiRNADendrimerPolyureaThe design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.Royal Society of ChemistryuBibliorumRestani, RitaConde, JoãoBaptista, PedroCidade, Maria Teresa VarandaBragança, AnaMorgado, JorgeCorreia, Ilídio Joaquim SobreiraRicardo, Ana AguiarBonifácio, Vasco2018-03-20T15:06:01Z2014-10-072014-10-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4663engRestani, R.B., Conde, J., Baptista, P.V., Cidade, M.T., Bragança, A.M., Morgado, J., Correia, I.J., Aguiar-Ricardo, A. e Bonifácio, V.D.B. (2014) “Polyurea dendrimer for efficient cytosolic siRNA delivery”, RSC Advances, Vol. 4(97): pp. 54872-54878metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:31:43Zoai:ubibliorum.ubi.pt:10400.6/4663Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:43.267578Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polyurea dendrimer for efficient cytosolic siRNA delivery
title Polyurea dendrimer for efficient cytosolic siRNA delivery
spellingShingle Polyurea dendrimer for efficient cytosolic siRNA delivery
Restani, Rita
SiRNA
Dendrimer
Polyurea
title_short Polyurea dendrimer for efficient cytosolic siRNA delivery
title_full Polyurea dendrimer for efficient cytosolic siRNA delivery
title_fullStr Polyurea dendrimer for efficient cytosolic siRNA delivery
title_full_unstemmed Polyurea dendrimer for efficient cytosolic siRNA delivery
title_sort Polyurea dendrimer for efficient cytosolic siRNA delivery
author Restani, Rita
author_facet Restani, Rita
Conde, João
Baptista, Pedro
Cidade, Maria Teresa Varanda
Bragança, Ana
Morgado, Jorge
Correia, Ilídio Joaquim Sobreira
Ricardo, Ana Aguiar
Bonifácio, Vasco
author_role author
author2 Conde, João
Baptista, Pedro
Cidade, Maria Teresa Varanda
Bragança, Ana
Morgado, Jorge
Correia, Ilídio Joaquim Sobreira
Ricardo, Ana Aguiar
Bonifácio, Vasco
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Restani, Rita
Conde, João
Baptista, Pedro
Cidade, Maria Teresa Varanda
Bragança, Ana
Morgado, Jorge
Correia, Ilídio Joaquim Sobreira
Ricardo, Ana Aguiar
Bonifácio, Vasco
dc.subject.por.fl_str_mv SiRNA
Dendrimer
Polyurea
topic SiRNA
Dendrimer
Polyurea
description The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-07
2014-10-07T00:00:00Z
2018-03-20T15:06:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4663
url http://hdl.handle.net/10400.6/4663
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Restani, R.B., Conde, J., Baptista, P.V., Cidade, M.T., Bragança, A.M., Morgado, J., Correia, I.J., Aguiar-Ricardo, A. e Bonifácio, V.D.B. (2014) “Polyurea dendrimer for efficient cytosolic siRNA delivery”, RSC Advances, Vol. 4(97): pp. 54872-54878
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rights_invalid_str_mv metadata only access
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dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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