β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/9495 |
Resumo: | The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of β-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4–8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process. |
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β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicusAspergillusβ-FructofuranosidaseFermentationFructooligosaccharidesImmobilizationbeta-FructofuranosidaseScience & TechnologyThe fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of β-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4–8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process.The financial support from FCT, the Portuguese Foundation for Science and Technology (research project SFRH/BPD/38212/2007) is gratefully acknowledged.Society for Industrial MicrobiologyUniversidade do MinhoMussatto, Solange I.Rodrigues, L. R.Teixeira, J. A.2009-072009-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9495eng"Journal of Industrial Microbiology and Biotechnology". ISSN 1367-5435. 36:7 (July 2008) 923-928.1367-543510.1007/s10295-009-0570-719396483info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:26Zoai:repositorium.sdum.uminho.pt:1822/9495Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:15.858847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
title |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
spellingShingle |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus Mussatto, Solange I. Aspergillus β-Fructofuranosidase Fermentation Fructooligosaccharides Immobilization beta-Fructofuranosidase Science & Technology |
title_short |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
title_full |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
title_fullStr |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
title_full_unstemmed |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
title_sort |
β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus |
author |
Mussatto, Solange I. |
author_facet |
Mussatto, Solange I. Rodrigues, L. R. Teixeira, J. A. |
author_role |
author |
author2 |
Rodrigues, L. R. Teixeira, J. A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Mussatto, Solange I. Rodrigues, L. R. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
Aspergillus β-Fructofuranosidase Fermentation Fructooligosaccharides Immobilization beta-Fructofuranosidase Science & Technology |
topic |
Aspergillus β-Fructofuranosidase Fermentation Fructooligosaccharides Immobilization beta-Fructofuranosidase Science & Technology |
description |
The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of β-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4–8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07 2009-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/9495 |
url |
http://hdl.handle.net/1822/9495 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Industrial Microbiology and Biotechnology". ISSN 1367-5435. 36:7 (July 2008) 923-928. 1367-5435 10.1007/s10295-009-0570-7 19396483 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society for Industrial Microbiology |
publisher.none.fl_str_mv |
Society for Industrial Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132543941869568 |