Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22208 |
Resumo: | The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 10 5, 2 × 10 6, or 2 × 10 7 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 10 7 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production. |
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Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditionsCoffee silverskinSolid-state fermentationFructooligosaccharidesβ-FructofuranosidaseAspergillus japonicusbeta-FructofuranosidaseScience & TechnologyThe conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 10 5, 2 × 10 6, or 2 × 10 7 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 10 7 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production.This work was supported by grant SFRH/BPD/38212/2007 from the FCT (Portuguese Foundation for Science and Technology).SpringerUniversidade do MinhoMussatto, Solange I.Ballesteros, Lina F.Martins, SilviaMaltos, Dulce A .F.Aguilar, Cristóbal N.Teixeira, J. A.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22208eng1935-513010.1007/s11947-012-0873-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:38Zoai:repositorium.sdum.uminho.pt:1822/22208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:08.111539Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
title |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
spellingShingle |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions Mussatto, Solange I. Coffee silverskin Solid-state fermentation Fructooligosaccharides β-Fructofuranosidase Aspergillus japonicus beta-Fructofuranosidase Science & Technology |
title_short |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
title_full |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
title_fullStr |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
title_full_unstemmed |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
title_sort |
Maximization of fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus under solid-state fermentation conditions |
author |
Mussatto, Solange I. |
author_facet |
Mussatto, Solange I. Ballesteros, Lina F. Martins, Silvia Maltos, Dulce A .F. Aguilar, Cristóbal N. Teixeira, J. A. |
author_role |
author |
author2 |
Ballesteros, Lina F. Martins, Silvia Maltos, Dulce A .F. Aguilar, Cristóbal N. Teixeira, J. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Mussatto, Solange I. Ballesteros, Lina F. Martins, Silvia Maltos, Dulce A .F. Aguilar, Cristóbal N. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
Coffee silverskin Solid-state fermentation Fructooligosaccharides β-Fructofuranosidase Aspergillus japonicus beta-Fructofuranosidase Science & Technology |
topic |
Coffee silverskin Solid-state fermentation Fructooligosaccharides β-Fructofuranosidase Aspergillus japonicus beta-Fructofuranosidase Science & Technology |
description |
The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 10 5, 2 × 10 6, or 2 × 10 7 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 10 7 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22208 |
url |
http://hdl.handle.net/1822/22208 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1935-5130 10.1007/s11947-012-0873-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133058048196609 |