Hemes on a string
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/164426 |
Resumo: | Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT ( ROTEIRO/0031/2013 and PINFRA/22161/2016 ) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Funding Information: Elisabete Ferreira (BioLab, UCIBIO, FCT-NOVA) is acknowledged for the technical assistance with the circular dichroism and differential scanning calorimetry experiments. T. M. F. L. M. and C. A. S. conceptualization; T. M. F. M. A. S. L. M. and C. A. S. investigation; T. M. F. M. A. S. L. M. formal analysis; T. M. F. writing–original draft; T. M. F. M. A. S. L. M. and C. A. S. writing–review & editing; T. M. F. visualization; L. M. and C. A. S. supervision; L. M. and C. A. S. funding acquisition; C. A. S. project administration. This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013 and PINFRA/22161/2016) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © 2023 The Authors |
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Hemes on a stringinsights on the functional mechanisms of PgcA from Geobacter sulfurreducenselectron transferGeobacterMetalloproteinmultiheme cytochromesnuclear magnetic resonance (NMR)protein domainRespiratory chainBiochemistryMolecular BiologyCell BiologyFunding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT ( ROTEIRO/0031/2013 and PINFRA/22161/2016 ) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Funding Information: Elisabete Ferreira (BioLab, UCIBIO, FCT-NOVA) is acknowledged for the technical assistance with the circular dichroism and differential scanning calorimetry experiments. T. M. F. L. M. and C. A. S. conceptualization; T. M. F. M. A. S. L. M. and C. A. S. investigation; T. M. F. M. A. S. L. M. formal analysis; T. M. F. writing–original draft; T. M. F. M. A. S. L. M. and C. A. S. writing–review & editing; T. M. F. visualization; L. M. and C. A. S. supervision; L. M. and C. A. S. funding acquisition; C. A. S. project administration. This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013 and PINFRA/22161/2016) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © 2023 The AuthorsMicrobial extracellular reduction of insoluble compounds requires soluble electron shuttles that diffuse in the environment, freely diffusing cytochromes, or direct contact with cellular conductive appendages that release or harvest electrons to assure a continuous balance between cellular requirements and environmental conditions. In this work, we produced and characterized the three cytochrome domains of PgcA, an extracellular triheme cytochrome that contributes to Fe(III) and Mn(IV) oxides reduction in Geobacter sulfurreducens. The three monoheme domains are structurally homologous, but their heme groups show variable axial coordination and reduction potential values. Electron transfer experiments monitored by NMR and visible spectroscopy show the variable extent to which the domains promiscuously exchange electrons while reducing different electron acceptors. The results suggest that PgcA is part of a new class of cytochromes - microbial heme-tethered redox strings - that use low-complexity protein stretches to bind metals and promote intra- and intermolecular electron transfer events through its cytochrome domains.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNFernandes, Tomás M.Silva, Marta A.Morgado, LeonorSalgueiro, Carlos A.2024-03-04T23:56:17Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/164426eng0021-9258PURE: 83887589https://doi.org/10.1016/j.jbc.2023.105167info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:52:09Zoai:run.unl.pt:10362/164426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:09.950810Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Hemes on a string insights on the functional mechanisms of PgcA from Geobacter sulfurreducens |
| title |
Hemes on a string |
| spellingShingle |
Hemes on a string Fernandes, Tomás M. electron transfer Geobacter Metalloprotein multiheme cytochromes nuclear magnetic resonance (NMR) protein domain Respiratory chain Biochemistry Molecular Biology Cell Biology |
| title_short |
Hemes on a string |
| title_full |
Hemes on a string |
| title_fullStr |
Hemes on a string |
| title_full_unstemmed |
Hemes on a string |
| title_sort |
Hemes on a string |
| author |
Fernandes, Tomás M. |
| author_facet |
Fernandes, Tomás M. Silva, Marta A. Morgado, Leonor Salgueiro, Carlos A. |
| author_role |
author |
| author2 |
Silva, Marta A. Morgado, Leonor Salgueiro, Carlos A. |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Fernandes, Tomás M. Silva, Marta A. Morgado, Leonor Salgueiro, Carlos A. |
| dc.subject.por.fl_str_mv |
electron transfer Geobacter Metalloprotein multiheme cytochromes nuclear magnetic resonance (NMR) protein domain Respiratory chain Biochemistry Molecular Biology Cell Biology |
| topic |
electron transfer Geobacter Metalloprotein multiheme cytochromes nuclear magnetic resonance (NMR) protein domain Respiratory chain Biochemistry Molecular Biology Cell Biology |
| description |
Funding Information: This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT ( ROTEIRO/0031/2013 and PINFRA/22161/2016 ) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Funding Information: Elisabete Ferreira (BioLab, UCIBIO, FCT-NOVA) is acknowledged for the technical assistance with the circular dichroism and differential scanning calorimetry experiments. T. M. F. L. M. and C. A. S. conceptualization; T. M. F. M. A. S. L. M. and C. A. S. investigation; T. M. F. M. A. S. L. M. formal analysis; T. M. F. writing–original draft; T. M. F. M. A. S. L. M. and C. A. S. writing–review & editing; T. M. F. visualization; L. M. and C. A. S. supervision; L. M. and C. A. S. funding acquisition; C. A. S. project administration. This work was supported by Fundação para a Ciência e Tecnologia (FCT) through the following grants: SFRH/BD/145039/2019 (T. M. F.), EXPL/BIA-BQM/0770/2021 (L. M.) and PTDC/BIABQM/4967/2020 (C. A. S.). This work was also supported by national funds from FCT in the scope of the projects (i) UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences – UCIBIO) and (ii) LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy – i4HB). The NMR spectrometers are part of the National NMR Network and are supported by FCT (ROTEIRO/0031/2013 and PINFRA/22161/2016) cofounded by FEDER through COMPETE 2020, POCI, PORL and FCT through PIDDAC. Publisher Copyright: © 2023 The Authors |
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2023 |
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2023-10 2023-10-01T00:00:00Z 2024-03-04T23:56:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/10362/164426 |
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eng |
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0021-9258 PURE: 83887589 https://doi.org/10.1016/j.jbc.2023.105167 |
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