Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/152858 |
Resumo: | Cancer is one of the most common causes of death in the world and current treatments are usually insufficient and present many gaps, leading to a will to cross the limitations and find new methods of treatment. Thereby, the development of new cancer treatment options is one of the most studied research areas, particularly in development of stimuliresponsive systems for localized and controlled drug delivery systems. The incorporation of magnetic nanoparticles (MNPs) in thermoresponsive nanofibers enables the development of dual responsive systems with significant applications in cancer therapy such as magnetic hyperthermia and controlled drug delivery. This thesis’ objective is to engineer a stimuli-responsive nanofibrous membrane for cancer treatment. To reach this purpose, a thermoresponsive polymer (p(MEO2MA-co- OEGMA)) was processed, optimized and characterized with the aim to produce electrospun nanofibers with the ability to respond to temperature in the ranges of magnetic hyperthermia (42-45 °C). The thermoresponsive behaviour of the p(MEO2MA-co- OEGMA)89:11 copolymer shown the better results to the purpose, presenting a lower critical solution temperature of around 42°C. The p(MEO2MA-co-OEGMA)89:11 copolymer could not be electrospun by itself. Therefore, two auxiliary polymers were used to evaluate the electrospinning abillity and to produce fibers with p(MEO2MA-co-OEGMA)89:11. Solutions of polyethylene oxide were prepared but the results of the electrospinning of a control fiberwere not adequate. Therefore, the choice of poly(vinyl alcohol) (10% (w/v)), whose electrospinning parameters were already optimized, was the best fit for the production of smart fibers using the thermoresponsive p(MEO2MA-co-OEGMA)89:11 copolymer. Lastly, after the optimization of electrospinning parameters, the electrospun nanofibers were characterized in terms of morphology to evaluate fiber diameter and homogeneity. PVA-p(MEO2MA-co-OEGMA)89:11 membranes with a ratio of 1:1 of PVA p(MEO2MA-co- OEGMA)89:11, respectively, and presented the most adequate results. PVA-p(MEO2MA-co-OEGMA)89:11 membranes presenting a lower critical solution temperature in the range of hyperthermic temperatures (42-45 °C) show, for this reason, potential to be used in alternative cancer treatment applications. |
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Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatmentCancerElectrospinningThermoresponsive polymerDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasCancer is one of the most common causes of death in the world and current treatments are usually insufficient and present many gaps, leading to a will to cross the limitations and find new methods of treatment. Thereby, the development of new cancer treatment options is one of the most studied research areas, particularly in development of stimuliresponsive systems for localized and controlled drug delivery systems. The incorporation of magnetic nanoparticles (MNPs) in thermoresponsive nanofibers enables the development of dual responsive systems with significant applications in cancer therapy such as magnetic hyperthermia and controlled drug delivery. This thesis’ objective is to engineer a stimuli-responsive nanofibrous membrane for cancer treatment. To reach this purpose, a thermoresponsive polymer (p(MEO2MA-co- OEGMA)) was processed, optimized and characterized with the aim to produce electrospun nanofibers with the ability to respond to temperature in the ranges of magnetic hyperthermia (42-45 °C). The thermoresponsive behaviour of the p(MEO2MA-co- OEGMA)89:11 copolymer shown the better results to the purpose, presenting a lower critical solution temperature of around 42°C. The p(MEO2MA-co-OEGMA)89:11 copolymer could not be electrospun by itself. Therefore, two auxiliary polymers were used to evaluate the electrospinning abillity and to produce fibers with p(MEO2MA-co-OEGMA)89:11. Solutions of polyethylene oxide were prepared but the results of the electrospinning of a control fiberwere not adequate. Therefore, the choice of poly(vinyl alcohol) (10% (w/v)), whose electrospinning parameters were already optimized, was the best fit for the production of smart fibers using the thermoresponsive p(MEO2MA-co-OEGMA)89:11 copolymer. Lastly, after the optimization of electrospinning parameters, the electrospun nanofibers were characterized in terms of morphology to evaluate fiber diameter and homogeneity. PVA-p(MEO2MA-co-OEGMA)89:11 membranes with a ratio of 1:1 of PVA p(MEO2MA-co- OEGMA)89:11, respectively, and presented the most adequate results. PVA-p(MEO2MA-co-OEGMA)89:11 membranes presenting a lower critical solution temperature in the range of hyperthermic temperatures (42-45 °C) show, for this reason, potential to be used in alternative cancer treatment applications.O cancro é uma das causas de morte mais comuns no mundo, sendo que os tratamentos atualmente existentes são normalmente insuficientes e apresentam muitas lacunas, levando a uma vontade de encontrar novos métodos de tratamento. Por conseguinte, o desenvolvimento de novas opções de tratamento do cancro é uma das áreas de investigação mais estudadas, particularmente no que diz respeito ao desenvolvimento de sistemas com capacidade de resposta a múltiplos estímulos para aplicações de libertação localizada e controlada de fármaco. A incorporação de nanopartículas magnéticas em fibras termossensíveis permite o desenvolvimento de sistemas com capacidade de resposta a dois estímulos diferentes, importantes para o tratamento de cancro. O objectivo desta tese é produzir uma membrana termossensível para o tratamento de cancro. Para alcançar este propósito, um polímero termossensível recentemente abordado na literatura (p(MEO2MA-co-OEGMA)) foi produzido, optimizado e caracterizado com o objectivo de produzir fibras, por eletrofiação, sensíveis a temperaturas no intervalo da hipertermia magnética (42-45 °C). O comportamento do copolímero p(MEO2MA-co- OEGMA)89:11 face à temperatura foi o ideal para o propósito, apresentando uma temperatura de transição em solução de cerca de 42°C. A produção de fibras do copolímero p(MEO2MA-co-OEGMA)89:11 por si só não foi possível, pelo que se escolheram dois polímeros para facilitar o processo de eletrofiação do copolímero em questão. Deste modo, prepararam-se soluções de óxido de polietileno no entanto, os resultados obtidos aquando do eletrofiação de uma membrana de controlo não foram favoráveis. Por isso, a escolha de outro polímero recaiu sobre o álcool polivinílico (10% (m/v)), cujos parâmetros de eletrofiação tinham já sido optimizados, permitindo assim a produção de fibras termossensíveis de p(MEO2MA-co-OEGMA)89:11. Finalmente, após a optimização dos parâmetros de eletrofiação, as nanofibras obtidas foram caracterizadas e analisadas. As fibras de PVA-p(MEO2MA-co-OEGMA)89:11 com um rácio de 1 para 1 apresentaram os resultados mais favoráveis e adequados para o pretendido, com uma temperatura de transição em solução na ordem das temperaturas da hipertermia magnética (42-45 °C).Soares, PaulaBorges, JoãoRUNOliveira, Leonor Nunes da Cunha Castro2023-05-17T14:06:51Z2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/152858enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:35:24Zoai:run.unl.pt:10362/152858Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:05.446868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
title |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
spellingShingle |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment Oliveira, Leonor Nunes da Cunha Castro Cancer Electrospinning Thermoresponsive polymer Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
title_full |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
title_fullStr |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
title_full_unstemmed |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
title_sort |
Engineering a multi-stimulus responsive nanofibrous membrane for cancer treatment |
author |
Oliveira, Leonor Nunes da Cunha Castro |
author_facet |
Oliveira, Leonor Nunes da Cunha Castro |
author_role |
author |
dc.contributor.none.fl_str_mv |
Soares, Paula Borges, João RUN |
dc.contributor.author.fl_str_mv |
Oliveira, Leonor Nunes da Cunha Castro |
dc.subject.por.fl_str_mv |
Cancer Electrospinning Thermoresponsive polymer Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Cancer Electrospinning Thermoresponsive polymer Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
Cancer is one of the most common causes of death in the world and current treatments are usually insufficient and present many gaps, leading to a will to cross the limitations and find new methods of treatment. Thereby, the development of new cancer treatment options is one of the most studied research areas, particularly in development of stimuliresponsive systems for localized and controlled drug delivery systems. The incorporation of magnetic nanoparticles (MNPs) in thermoresponsive nanofibers enables the development of dual responsive systems with significant applications in cancer therapy such as magnetic hyperthermia and controlled drug delivery. This thesis’ objective is to engineer a stimuli-responsive nanofibrous membrane for cancer treatment. To reach this purpose, a thermoresponsive polymer (p(MEO2MA-co- OEGMA)) was processed, optimized and characterized with the aim to produce electrospun nanofibers with the ability to respond to temperature in the ranges of magnetic hyperthermia (42-45 °C). The thermoresponsive behaviour of the p(MEO2MA-co- OEGMA)89:11 copolymer shown the better results to the purpose, presenting a lower critical solution temperature of around 42°C. The p(MEO2MA-co-OEGMA)89:11 copolymer could not be electrospun by itself. Therefore, two auxiliary polymers were used to evaluate the electrospinning abillity and to produce fibers with p(MEO2MA-co-OEGMA)89:11. Solutions of polyethylene oxide were prepared but the results of the electrospinning of a control fiberwere not adequate. Therefore, the choice of poly(vinyl alcohol) (10% (w/v)), whose electrospinning parameters were already optimized, was the best fit for the production of smart fibers using the thermoresponsive p(MEO2MA-co-OEGMA)89:11 copolymer. Lastly, after the optimization of electrospinning parameters, the electrospun nanofibers were characterized in terms of morphology to evaluate fiber diameter and homogeneity. PVA-p(MEO2MA-co-OEGMA)89:11 membranes with a ratio of 1:1 of PVA p(MEO2MA-co- OEGMA)89:11, respectively, and presented the most adequate results. PVA-p(MEO2MA-co-OEGMA)89:11 membranes presenting a lower critical solution temperature in the range of hyperthermic temperatures (42-45 °C) show, for this reason, potential to be used in alternative cancer treatment applications. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10 2022-10-01T00:00:00Z 2023-05-17T14:06:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://hdl.handle.net/10362/152858 |
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eng |
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eng |
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