Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/62355 |
Resumo: | Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens. |
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Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungiParasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.NatureRepositório da Universidade de LisboaFisher, Matthew C.Ghosh, PriaShelton, Jennifer M. G.Bates, KieranBrookes, LolaWierzbicki, ClaudiaRosa, Gonçalo M.Farrer, Rhys A.Aanensen, David M.Alvarado-Rybak, MarioBataille, ArnaudBerger, LeeBöll, SusanneBosch, JaimeClare, Frances C.A. Courtois, ElodieCrottini, AngelicaCunningham, Andrew A.Doherty-Bone, Thomas M.Gebresenbet, FikirteGower, David J.Höglund, JacobJames, Timothy Y.Jenkinson, Thomas S.Kosch, Tiffany A.Lambertini, CarolinaLaurila, AnssiLin, Chun-FuLoyau, AdelineMartel, AnMeurling, SaraMiaud, ClaudeMinting, PeteNdriantsoa, SergeO’Hanlon, Simon J.Pasmans, FrankRakotonanahary, TsantaRabemananjara, Falitiana C. E.Ribeiro, Luisa P.Schmeller, Dirk S.Schmidt, Benedikt R.Skerratt, LeeSmith, FreyaSoto-Azat, ClaudioTessa, GiuliaToledo, Luís FelipeValenzuela-Sánchez, AndrésVerster, RuhanVörös, JuditWaldman, BruceWebb, Rebecca J.Weldon, CheWombwell, EmmaZamudio, Kelly R.Longcore, Joyce E.Garner, Trenton W. J.2024-01-31T18:44:29Z2018-052018-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/62355engFisher, M. C., Ghosh, P., Shelton, J., Bates, K. A., Brookes, L., Wierzbicki, C., Rosa, G. M., Farrer, R. A., Aanensen, D. M., Alvarado‐Rybak, M., Bataillé, A., Berger, L., Böll, S., Bosch, J., Clare, F. C., Courtois, E. A., Crottini, A., Cunningham, A. A., Doherty‐Bone, T. M., . . . Garner, T. W. J. (2018). Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-24472-210.1038/s41598-018-24472-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-05T01:23:42Zoai:repositorio.ul.pt:10451/62355Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:08:30.581332Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
title |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
spellingShingle |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi Fisher, Matthew C. |
title_short |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
title_full |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
title_fullStr |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
title_full_unstemmed |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
title_sort |
Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
author |
Fisher, Matthew C. |
author_facet |
Fisher, Matthew C. Ghosh, Pria Shelton, Jennifer M. G. Bates, Kieran Brookes, Lola Wierzbicki, Claudia Rosa, Gonçalo M. Farrer, Rhys A. Aanensen, David M. Alvarado-Rybak, Mario Bataille, Arnaud Berger, Lee Böll, Susanne Bosch, Jaime Clare, Frances C. A. Courtois, Elodie Crottini, Angelica Cunningham, Andrew A. Doherty-Bone, Thomas M. Gebresenbet, Fikirte Gower, David J. Höglund, Jacob James, Timothy Y. Jenkinson, Thomas S. Kosch, Tiffany A. Lambertini, Carolina Laurila, Anssi Lin, Chun-Fu Loyau, Adeline Martel, An Meurling, Sara Miaud, Claude Minting, Pete Ndriantsoa, Serge O’Hanlon, Simon J. Pasmans, Frank Rakotonanahary, Tsanta Rabemananjara, Falitiana C. E. Ribeiro, Luisa P. Schmeller, Dirk S. Schmidt, Benedikt R. Skerratt, Lee Smith, Freya Soto-Azat, Claudio Tessa, Giulia Toledo, Luís Felipe Valenzuela-Sánchez, Andrés Verster, Ruhan Vörös, Judit Waldman, Bruce Webb, Rebecca J. Weldon, Che Wombwell, Emma Zamudio, Kelly R. Longcore, Joyce E. Garner, Trenton W. J. |
author_role |
author |
author2 |
Ghosh, Pria Shelton, Jennifer M. G. Bates, Kieran Brookes, Lola Wierzbicki, Claudia Rosa, Gonçalo M. Farrer, Rhys A. Aanensen, David M. Alvarado-Rybak, Mario Bataille, Arnaud Berger, Lee Böll, Susanne Bosch, Jaime Clare, Frances C. A. Courtois, Elodie Crottini, Angelica Cunningham, Andrew A. Doherty-Bone, Thomas M. Gebresenbet, Fikirte Gower, David J. Höglund, Jacob James, Timothy Y. Jenkinson, Thomas S. Kosch, Tiffany A. Lambertini, Carolina Laurila, Anssi Lin, Chun-Fu Loyau, Adeline Martel, An Meurling, Sara Miaud, Claude Minting, Pete Ndriantsoa, Serge O’Hanlon, Simon J. Pasmans, Frank Rakotonanahary, Tsanta Rabemananjara, Falitiana C. E. Ribeiro, Luisa P. Schmeller, Dirk S. Schmidt, Benedikt R. Skerratt, Lee Smith, Freya Soto-Azat, Claudio Tessa, Giulia Toledo, Luís Felipe Valenzuela-Sánchez, Andrés Verster, Ruhan Vörös, Judit Waldman, Bruce Webb, Rebecca J. Weldon, Che Wombwell, Emma Zamudio, Kelly R. Longcore, Joyce E. Garner, Trenton W. J. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Fisher, Matthew C. Ghosh, Pria Shelton, Jennifer M. G. Bates, Kieran Brookes, Lola Wierzbicki, Claudia Rosa, Gonçalo M. Farrer, Rhys A. Aanensen, David M. Alvarado-Rybak, Mario Bataille, Arnaud Berger, Lee Böll, Susanne Bosch, Jaime Clare, Frances C. A. Courtois, Elodie Crottini, Angelica Cunningham, Andrew A. Doherty-Bone, Thomas M. Gebresenbet, Fikirte Gower, David J. Höglund, Jacob James, Timothy Y. Jenkinson, Thomas S. Kosch, Tiffany A. Lambertini, Carolina Laurila, Anssi Lin, Chun-Fu Loyau, Adeline Martel, An Meurling, Sara Miaud, Claude Minting, Pete Ndriantsoa, Serge O’Hanlon, Simon J. Pasmans, Frank Rakotonanahary, Tsanta Rabemananjara, Falitiana C. E. Ribeiro, Luisa P. Schmeller, Dirk S. Schmidt, Benedikt R. Skerratt, Lee Smith, Freya Soto-Azat, Claudio Tessa, Giulia Toledo, Luís Felipe Valenzuela-Sánchez, Andrés Verster, Ruhan Vörös, Judit Waldman, Bruce Webb, Rebecca J. Weldon, Che Wombwell, Emma Zamudio, Kelly R. Longcore, Joyce E. Garner, Trenton W. J. |
description |
Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-05 2018-05-01T00:00:00Z 2024-01-31T18:44:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/62355 |
url |
http://hdl.handle.net/10451/62355 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fisher, M. C., Ghosh, P., Shelton, J., Bates, K. A., Brookes, L., Wierzbicki, C., Rosa, G. M., Farrer, R. A., Aanensen, D. M., Alvarado‐Rybak, M., Bataillé, A., Berger, L., Böll, S., Bosch, J., Clare, F. C., Courtois, E. A., Crottini, A., Cunningham, A. A., Doherty‐Bone, T. M., . . . Garner, T. W. J. (2018). Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-24472-2 10.1038/s41598-018-24472-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Nature |
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Nature |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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