Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology

Detalhes bibliográficos
Autor(a) principal: S., Ahamd
Data de Publicação: 2017
Outros Autores: F., Teresa, Saldanha, Carlota, H., Ana S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/34381
Resumo: Copyright: © 2017 Ahamd S.
id RCAP_face58acb760a2ae5292df3805bbd0b5
oai_identifier_str oai:repositorio.ul.pt:10451/34381
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheologyCopyright: © 2017 Ahamd S.Erythrocyte deformability is a biorheological influent factor on blood viscosity, cellular oxygenation and a biomarker of acute and chronic inflammation. An inverse relationship between erythrocyte ability to reversibly change its shape with nitric oxide (NO) efflux from erythrocytes was reported in patients with hypercholesterolemia, hypertension and renal transplant. Erythrocyte NO efflux is negatively associated with carotid intima-media thickness and an independent predictor of this structural anomaly. High concentrations of oxidized LDL when in presence of healthy human erythrocytes increase its ability to scavenge NO. The aim of this work was to evaluate in vitro, the effects on erythrocyte nitric oxide (NO) metabolism and its deformability under presence of lipoproteins LDL /HDL. Aliquots of healthy human blood samples were enriched, or not (control), with synthetic LDL/HDL solutions ([LDL/HDL]1=18.44/0.776 mg/dL and [LDL/HDL]2=36.88/1.552 mg/dL). NO efflux from erythrocyte and its content on nitrite, nitrate, S-nitrosoglutathione (GSNO), peroxynitrite and its deformability (EEI) were determined. So, from each blood sample three aliquots were made: the control and two different LDL/HDL concentrations. In the present study, were not observed variations on peroxynitrite levels inside erythrocytes, on the ability to scavenge NO and on EEI at all shear stress when compared to the values obtained in untreated samples. NO mobilization inside erythrocytes of the treated aliquots were showed by the significantly increased of nitrite, nitrate and S-nitrosoglutathione levels in relation to the control aliquots without significant differences between both treated aliquots. In this in vitro study with erythrocytes obtained from blood samples of healthy human we show that under LDL/HDL addition, maintenance of its membrane deformability, nitric oxide and the nitrogen redox level content. All these results evidence the ability of erythrocytes to answer LDL/HDL binding by increasing NO mobilization with higher nitrite, nitrate and GSNO concentrations. Better understanding of the signal transduction mechanism associated to the erythrocytes binding LDL/HDL receptors may bring potential targets in cases of diminished erythrocyte deformability or impeachment delivery of nitric oxide to alleviate the compromised microcirculation.Repositório da Universidade de LisboaS., AhamdF., TeresaSaldanha, CarlotaH., Ana S.2018-07-30T11:22:32Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/34381engClin Med Invest, 2017, Volume 2(4): 1-52398-576310.15761/CMI.1000141info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:43:53Zoai:repositorio.ul.pt:10451/34381Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:43:53Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
title Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
spellingShingle Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
S., Ahamd
title_short Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
title_full Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
title_fullStr Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
title_full_unstemmed Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
title_sort Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology
author S., Ahamd
author_facet S., Ahamd
F., Teresa
Saldanha, Carlota
H., Ana S.
author_role author
author2 F., Teresa
Saldanha, Carlota
H., Ana S.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv S., Ahamd
F., Teresa
Saldanha, Carlota
H., Ana S.
description Copyright: © 2017 Ahamd S.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-07-30T11:22:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/34381
url http://hdl.handle.net/10451/34381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clin Med Invest, 2017, Volume 2(4): 1-5
2398-5763
10.15761/CMI.1000141
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817549006245462016

Registros relacionados