Amperometric measurements of nitric oxide in erythrocytes

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena A.
Data de Publicação: 2004
Outros Autores: Martins-Silva, João, Carlota, Saldanha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/17629
Resumo: © 2004 Elsevier B.V. All rights reserved.
id RCAP_a8c8d7e9d7c79b5869addd066cc0cf4f
oai_identifier_str oai:repositorio.ul.pt:10451/17629
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Amperometric measurements of nitric oxide in erythrocytesNitric oxideNitric oxide sensorAmperometric methodErythrocytes© 2004 Elsevier B.V. All rights reserved.In the recent years, there has been an increase in the development of new biosensors that could be helpful in the study of various physiological processes. In this study, we report the development of a new in vitro experimental design for real-time nitric oxide (NO) amperometric measurements in erythrocyte suspensions. To achieve this, we employed human erythrocyte suspensions in sodium chloride 0.9%, pH 7 (haematocrit 0.05%). The production of NO by erythrocytes was measured with a commercial NO sensor during stimulation by l-arginine, acetylcholine, choline, atropine and velnacrine maleate (10 μM of final concentrations). We also measured the nitrite and nitrate concentrations produced by erythrocyte suspensions stimulated with the above effectors by means of the Griess reaction method. We observed that there was a direct relation between the electric current produced by the NO sensor, and the NO standard concentrations, thereby leading to a good calibration curve. The in vitro erythrocytes produced significant amperometric NO values in response to a wide range of effectors and these results have the same variation profile of the nitrites and nitrates results achieved with the Griess method. In conclusion, the amperometric NO sensor constitutes a reliable method for direct, and real-time measurement in vitro of the NO production of erythrocyte suspensions, As such, it offers a potential diagnostic technique for the evaluation of diseases, and the therapeutic progression of diseases, related to intracellular NO metabolism.This work was supported by the Faculdade de Medicina da Universidade de Lisboa and by the Fundação para a Ciência e Tecnologia, Ministério da Ciência e Tecnologia, Lisboa, Portugal.ElsevierRepositório da Universidade de LisboaCarvalho, Filomena A.Martins-Silva, JoãoCarlota, Saldanha2015-03-05T14:30:59Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17629engBiosensors and Bioelectronics 20 (2004) 505–5080956-5663http://dx.doi.org/10.1016/j.bios.2004.02.015metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:03:22Zoai:repositorio.ul.pt:10451/17629Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:37:27.140741Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Amperometric measurements of nitric oxide in erythrocytes
title Amperometric measurements of nitric oxide in erythrocytes
spellingShingle Amperometric measurements of nitric oxide in erythrocytes
Carvalho, Filomena A.
Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
title_short Amperometric measurements of nitric oxide in erythrocytes
title_full Amperometric measurements of nitric oxide in erythrocytes
title_fullStr Amperometric measurements of nitric oxide in erythrocytes
title_full_unstemmed Amperometric measurements of nitric oxide in erythrocytes
title_sort Amperometric measurements of nitric oxide in erythrocytes
author Carvalho, Filomena A.
author_facet Carvalho, Filomena A.
Martins-Silva, João
Carlota, Saldanha
author_role author
author2 Martins-Silva, João
Carlota, Saldanha
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena A.
Martins-Silva, João
Carlota, Saldanha
dc.subject.por.fl_str_mv Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
topic Nitric oxide
Nitric oxide sensor
Amperometric method
Erythrocytes
description © 2004 Elsevier B.V. All rights reserved.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2015-03-05T14:30:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/17629
url http://hdl.handle.net/10451/17629
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biosensors and Bioelectronics 20 (2004) 505–508
0956-5663
http://dx.doi.org/10.1016/j.bios.2004.02.015
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134272200638464

Registros relacionados