Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation

Detalhes bibliográficos
Autor(a) principal: Franklin, Gregory
Data de Publicação: 2008
Outros Autores: Conceição, Luís F. R., Kombrink, E., Dias, Alberto Carlos Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/10495
Resumo: Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.
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spelling Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivationAgrobacteriumHypericumPlant defense responsePlant recalcitranceScience & TechnologyPlant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.Fundação para a Ciência e a Tecnologia (FCT) - POCTI/AGR/40283/2001, SFRH/BPD/17102/ 2004Springer VerlagUniversidade do MinhoFranklin, GregoryConceição, Luís F. R.Kombrink, E.Dias, Alberto Carlos Pires20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10495eng"Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408.0032-093510.1007/s00425-008-0691-718247048The original publication is available at www.springerlink.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:55Zoai:repositorium.sdum.uminho.pt:1822/10495Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:14.945035Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
title Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
spellingShingle Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
Franklin, Gregory
Agrobacterium
Hypericum
Plant defense response
Plant recalcitrance
Science & Technology
title_short Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
title_full Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
title_fullStr Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
title_full_unstemmed Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
title_sort Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
author Franklin, Gregory
author_facet Franklin, Gregory
Conceição, Luís F. R.
Kombrink, E.
Dias, Alberto Carlos Pires
author_role author
author2 Conceição, Luís F. R.
Kombrink, E.
Dias, Alberto Carlos Pires
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Franklin, Gregory
Conceição, Luís F. R.
Kombrink, E.
Dias, Alberto Carlos Pires
dc.subject.por.fl_str_mv Agrobacterium
Hypericum
Plant defense response
Plant recalcitrance
Science & Technology
topic Agrobacterium
Hypericum
Plant defense response
Plant recalcitrance
Science & Technology
description Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/10495
url http://hdl.handle.net/1822/10495
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408.
0032-0935
10.1007/s00425-008-0691-7
18247048
The original publication is available at www.springerlink.com
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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