Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/10495 |
Resumo: | Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation. |
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Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivationAgrobacteriumHypericumPlant defense responsePlant recalcitranceScience & TechnologyPlant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.Fundação para a Ciência e a Tecnologia (FCT) - POCTI/AGR/40283/2001, SFRH/BPD/17102/ 2004Springer VerlagUniversidade do MinhoFranklin, GregoryConceição, Luís F. R.Kombrink, E.Dias, Alberto Carlos Pires20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10495eng"Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408.0032-093510.1007/s00425-008-0691-718247048The original publication is available at www.springerlink.cominfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:55Zoai:repositorium.sdum.uminho.pt:1822/10495Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:14.945035Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
title |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
spellingShingle |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation Franklin, Gregory Agrobacterium Hypericum Plant defense response Plant recalcitrance Science & Technology |
title_short |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
title_full |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
title_fullStr |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
title_full_unstemmed |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
title_sort |
Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
author |
Franklin, Gregory |
author_facet |
Franklin, Gregory Conceição, Luís F. R. Kombrink, E. Dias, Alberto Carlos Pires |
author_role |
author |
author2 |
Conceição, Luís F. R. Kombrink, E. Dias, Alberto Carlos Pires |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Franklin, Gregory Conceição, Luís F. R. Kombrink, E. Dias, Alberto Carlos Pires |
dc.subject.por.fl_str_mv |
Agrobacterium Hypericum Plant defense response Plant recalcitrance Science & Technology |
topic |
Agrobacterium Hypericum Plant defense response Plant recalcitrance Science & Technology |
description |
Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/10495 |
url |
http://hdl.handle.net/1822/10495 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408. 0032-0935 10.1007/s00425-008-0691-7 18247048 The original publication is available at www.springerlink.com |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132397012254720 |