Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/74263 |
Resumo: | Natural bioactive compounds from food waste have fomented interest in food and pharmaceutical industries for the past decade. In this work, it purposed the recovery of bioactive avocado peel extract using an environmentally friendly technique: the ultrasound assisted extraction. The response surface methodology was applied in order to optimize the conditions of the extraction, ethanol-water mixtures and time. The optimized extracts (ethanol 38.46%, 44.06 min, and 50 °C) were chemically characterized by HPLC-ESI-MS and FTIR. Its antioxidant ability, as well as, its effect on cell metabolic activity of normal (L929) and cancer (Caco-2, A549 and HeLa) cell lines were assessed. Aqueous ethanol extracts presented a high content in bioactive compounds with high antioxidant potential. The most representative class of the phenolic compounds found in the avocado peel extract were phenolic acids, such as hydroxybenzoic and hydroxycinnamic acids. Another important chemical group detected were the flavonoids, such as flavanols, flavanonols, flavones, flavanones and chalcone, phenylethanoids and lignans. In terms of its influence on the metabolic activity of normal and cancer cell lines, the extract does not significantly affect normal cells. On the other hand, it can negatively affect cancer cells, particularly HeLa cells. These results clearly demonstrated that ultrasound is a sustainable extraction technique, resulting in extracts with low toxicity in normal cells and with potential application in food, pharmaceutical or nutraceutical sectors. |
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Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applicationsAgri-food by-productsBioactive compoundsUltrasound assisted extractionOptimizationBiological propertiesScience & TechnologyNatural bioactive compounds from food waste have fomented interest in food and pharmaceutical industries for the past decade. In this work, it purposed the recovery of bioactive avocado peel extract using an environmentally friendly technique: the ultrasound assisted extraction. The response surface methodology was applied in order to optimize the conditions of the extraction, ethanol-water mixtures and time. The optimized extracts (ethanol 38.46%, 44.06 min, and 50 °C) were chemically characterized by HPLC-ESI-MS and FTIR. Its antioxidant ability, as well as, its effect on cell metabolic activity of normal (L929) and cancer (Caco-2, A549 and HeLa) cell lines were assessed. Aqueous ethanol extracts presented a high content in bioactive compounds with high antioxidant potential. The most representative class of the phenolic compounds found in the avocado peel extract were phenolic acids, such as hydroxybenzoic and hydroxycinnamic acids. Another important chemical group detected were the flavonoids, such as flavanols, flavanonols, flavones, flavanones and chalcone, phenylethanoids and lignans. In terms of its influence on the metabolic activity of normal and cancer cell lines, the extract does not significantly affect normal cells. On the other hand, it can negatively affect cancer cells, particularly HeLa cells. These results clearly demonstrated that ultrasound is a sustainable extraction technique, resulting in extracts with low toxicity in normal cells and with potential application in food, pharmaceutical or nutraceutical sectors.The authors acknowledge the financial support received from “Xunta de Galicia” (GRC ED431C 2017/62-GRC, and Project ED431F 2020/03). These projects are partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). This research was also funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. Beatriz Gullón would like to express her gratitude to the Spanish Ministry of Economy and Competitiveness for her postdoctoral grant (Reference RYC2018-026177-I).info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoRodríguez-Martínez, BeatrizFerreira-Santos, P.Gullón, BeatrizTeixeira, J. A.Botelho, Cláudia M.Yáñez, Remedios2021-092021-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/74263engRodríguez-Martínez, Beatriz; Ferreira-Santos, P.; Gullón, Beatriz; Teixeira, José A.; Botelho, Cláudia M.; Yáñez, Remedios, Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications. Antioxidants, 10(9), 1475, 20212076-392110.3390/antiox10091475https://www.mdpi.com/2076-3921/10/9/1475info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:01Zoai:repositorium.sdum.uminho.pt:1822/74263Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:49:47.739636Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
title |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
spellingShingle |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications Rodríguez-Martínez, Beatriz Agri-food by-products Bioactive compounds Ultrasound assisted extraction Optimization Biological properties Science & Technology |
title_short |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
title_full |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
title_fullStr |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
title_full_unstemmed |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
title_sort |
Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications |
author |
Rodríguez-Martínez, Beatriz |
author_facet |
Rodríguez-Martínez, Beatriz Ferreira-Santos, P. Gullón, Beatriz Teixeira, J. A. Botelho, Cláudia M. Yáñez, Remedios |
author_role |
author |
author2 |
Ferreira-Santos, P. Gullón, Beatriz Teixeira, J. A. Botelho, Cláudia M. Yáñez, Remedios |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodríguez-Martínez, Beatriz Ferreira-Santos, P. Gullón, Beatriz Teixeira, J. A. Botelho, Cláudia M. Yáñez, Remedios |
dc.subject.por.fl_str_mv |
Agri-food by-products Bioactive compounds Ultrasound assisted extraction Optimization Biological properties Science & Technology |
topic |
Agri-food by-products Bioactive compounds Ultrasound assisted extraction Optimization Biological properties Science & Technology |
description |
Natural bioactive compounds from food waste have fomented interest in food and pharmaceutical industries for the past decade. In this work, it purposed the recovery of bioactive avocado peel extract using an environmentally friendly technique: the ultrasound assisted extraction. The response surface methodology was applied in order to optimize the conditions of the extraction, ethanol-water mixtures and time. The optimized extracts (ethanol 38.46%, 44.06 min, and 50 °C) were chemically characterized by HPLC-ESI-MS and FTIR. Its antioxidant ability, as well as, its effect on cell metabolic activity of normal (L929) and cancer (Caco-2, A549 and HeLa) cell lines were assessed. Aqueous ethanol extracts presented a high content in bioactive compounds with high antioxidant potential. The most representative class of the phenolic compounds found in the avocado peel extract were phenolic acids, such as hydroxybenzoic and hydroxycinnamic acids. Another important chemical group detected were the flavonoids, such as flavanols, flavanonols, flavones, flavanones and chalcone, phenylethanoids and lignans. In terms of its influence on the metabolic activity of normal and cancer cell lines, the extract does not significantly affect normal cells. On the other hand, it can negatively affect cancer cells, particularly HeLa cells. These results clearly demonstrated that ultrasound is a sustainable extraction technique, resulting in extracts with low toxicity in normal cells and with potential application in food, pharmaceutical or nutraceutical sectors. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09 2021-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/74263 |
url |
http://hdl.handle.net/1822/74263 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodríguez-Martínez, Beatriz; Ferreira-Santos, P.; Gullón, Beatriz; Teixeira, José A.; Botelho, Cláudia M.; Yáñez, Remedios, Exploiting the potential of bioactive molecules extracted by ultrasounds from avocado peelsfood and nutraceutical applications. Antioxidants, 10(9), 1475, 2021 2076-3921 10.3390/antiox10091475 https://www.mdpi.com/2076-3921/10/9/1475 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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