Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey
Autor(a) principal: | |
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Data de Publicação: | 1994 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10306 https://doi.org/10.1021/jf00045a027 |
Resumo: | Primary normal alcohols of 33 unpasteurized Galician (northwestern Spain) honeys have been determined as apparent ethanol contents. A modification of the Boehringer-Mannheim enzymatic method has been used. The solution of honey has been employed directly, neither clarified nor neutralized. Removal of interferences required absorbance measurements at 340 nm, by using the solution of honey with the solution of aldehyde dehydrogenase into the reference cuvette. Water is added to the reference cuvette and alcohol dehydrogenase suspension to the sample cuvette. The blanks are measured following the same procedure with redistilled water instead of sample solution. Ten replicate analyses of each of four samples with apparent ethanol levels of 13.5,35.3, 50.1, and 141.8 mgkg gave coefficient of variations of 1.74%, 0.48%, 0.34%, and 0.22%, respectively. The modified enzymatic method performed well in recovery experiments (recovery 100.1%). The apparent ethanol contents of 32 of the 33 honeys studied lay in the range 13.5-50.1 mgkg (mean 27.8 mgl kg); the remaining unspoiled honey had an apparent ethanol content of 141.8 mgkg. |
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Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in HoneyHoneyEthanolAlcoholsEnzymatic analysisPrimary normal alcohols of 33 unpasteurized Galician (northwestern Spain) honeys have been determined as apparent ethanol contents. A modification of the Boehringer-Mannheim enzymatic method has been used. The solution of honey has been employed directly, neither clarified nor neutralized. Removal of interferences required absorbance measurements at 340 nm, by using the solution of honey with the solution of aldehyde dehydrogenase into the reference cuvette. Water is added to the reference cuvette and alcohol dehydrogenase suspension to the sample cuvette. The blanks are measured following the same procedure with redistilled water instead of sample solution. Ten replicate analyses of each of four samples with apparent ethanol levels of 13.5,35.3, 50.1, and 141.8 mgkg gave coefficient of variations of 1.74%, 0.48%, 0.34%, and 0.22%, respectively. The modified enzymatic method performed well in recovery experiments (recovery 100.1%). The apparent ethanol contents of 32 of the 33 honeys studied lay in the range 13.5-50.1 mgkg (mean 27.8 mgl kg); the remaining unspoiled honey had an apparent ethanol content of 141.8 mgkg.American Chemical Society1994-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10306http://hdl.handle.net/10316/10306https://doi.org/10.1021/jf00045a027engJournal of Agricultural and Food Chemistry. 42:9 (1994) 1975-19780021-8561Huidobro, José F.Rea, M. EstrellaAndrade, Paula C. Branquinho deSanchez, M. P.Sancho, M. TeresaMuniategui, SoledadSimal-Lozano, Jesúsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-27T16:14:22Zoai:estudogeral.uc.pt:10316/10306Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:16.641097Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
title |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
spellingShingle |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey Huidobro, José F. Honey Ethanol Alcohols Enzymatic analysis |
title_short |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
title_full |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
title_fullStr |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
title_full_unstemmed |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
title_sort |
Enzymic Determination of Primary Normal Alcohols as Apparent Ethanol Content in Honey |
author |
Huidobro, José F. |
author_facet |
Huidobro, José F. Rea, M. Estrella Andrade, Paula C. Branquinho de Sanchez, M. P. Sancho, M. Teresa Muniategui, Soledad Simal-Lozano, Jesús |
author_role |
author |
author2 |
Rea, M. Estrella Andrade, Paula C. Branquinho de Sanchez, M. P. Sancho, M. Teresa Muniategui, Soledad Simal-Lozano, Jesús |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Huidobro, José F. Rea, M. Estrella Andrade, Paula C. Branquinho de Sanchez, M. P. Sancho, M. Teresa Muniategui, Soledad Simal-Lozano, Jesús |
dc.subject.por.fl_str_mv |
Honey Ethanol Alcohols Enzymatic analysis |
topic |
Honey Ethanol Alcohols Enzymatic analysis |
description |
Primary normal alcohols of 33 unpasteurized Galician (northwestern Spain) honeys have been determined as apparent ethanol contents. A modification of the Boehringer-Mannheim enzymatic method has been used. The solution of honey has been employed directly, neither clarified nor neutralized. Removal of interferences required absorbance measurements at 340 nm, by using the solution of honey with the solution of aldehyde dehydrogenase into the reference cuvette. Water is added to the reference cuvette and alcohol dehydrogenase suspension to the sample cuvette. The blanks are measured following the same procedure with redistilled water instead of sample solution. Ten replicate analyses of each of four samples with apparent ethanol levels of 13.5,35.3, 50.1, and 141.8 mgkg gave coefficient of variations of 1.74%, 0.48%, 0.34%, and 0.22%, respectively. The modified enzymatic method performed well in recovery experiments (recovery 100.1%). The apparent ethanol contents of 32 of the 33 honeys studied lay in the range 13.5-50.1 mgkg (mean 27.8 mgl kg); the remaining unspoiled honey had an apparent ethanol content of 141.8 mgkg. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10306 http://hdl.handle.net/10316/10306 https://doi.org/10.1021/jf00045a027 |
url |
http://hdl.handle.net/10316/10306 https://doi.org/10.1021/jf00045a027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry. 42:9 (1994) 1975-1978 0021-8561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133750060122113 |