Diversity and antimicrobial potential of bacteria isolated from Algarve caves

Detalhes bibliográficos
Autor(a) principal: Veloso, Telma Luísa Machado
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/25453
Resumo: Recently, the need for new molecules with antimicrobial activity increased due to a continuous the increasing number of multi drug resistant pathogenic bacteria. To fill this urging need, the scientific community has made a great investment in searching for new microorganisms and their bioactive compounds. A great part of the effort has been focused in environments with extreme conditions, since in these environments the inhabiting microorganisms must have very particular features. Karstic caves are considered extreme environments due to the nutrient scarcity caused by absence of natural light that precludes the growth of vascular green plants. In addition, the relatively low and stable temperature and the high humidity levels, force cave dwelling microorganisms to become highly specialized. The fact that these unique habitats are poorly studied, in particular when considering the bacterial community, makes them a potential reservoir for both new species and new antimicrobial compounds, as demonstrated in the few existing studies. Considering these facts our study aimed at: i) obtain and identify bacterial isolates sampled in three caves of Algarve, namely Vale do Telheiro, Senhora and Ibne Ammar and, ii) assess their antimicrobial activity. From the collected samples of the three caves we are able to isolate 110 bacterial isolates. After sequencing and searching for homologies in the databases it was possible to include the isolates in phyla, namely Firmicutes, Proteobacteria and Actinobacteria, in a total of 19 genera. Regarding the antimicrobial activity, the tests were performed against six test agents: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Aeromonas salmonicida ATCC 33658 and Bacillus cereus ATCC 14579. The results revealed that approximately 52% of the bacterial isolates presented antimicrobial activity against at least one test agent, and that ten bacterial isolates were able to inhibit the growth of more than four test agents. This study was the first to focus cultivable bacterial diversity in the Algarve caves and confirmed that prospection for new antimicrobial compounds in subterranean environments might be one of the strategies for fighting the problem of multi drug resistant bacteria.
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spelling Diversity and antimicrobial potential of bacteria isolated from Algarve cavesCavesDiversityBacteriaAntimicrobial activityRecently, the need for new molecules with antimicrobial activity increased due to a continuous the increasing number of multi drug resistant pathogenic bacteria. To fill this urging need, the scientific community has made a great investment in searching for new microorganisms and their bioactive compounds. A great part of the effort has been focused in environments with extreme conditions, since in these environments the inhabiting microorganisms must have very particular features. Karstic caves are considered extreme environments due to the nutrient scarcity caused by absence of natural light that precludes the growth of vascular green plants. In addition, the relatively low and stable temperature and the high humidity levels, force cave dwelling microorganisms to become highly specialized. The fact that these unique habitats are poorly studied, in particular when considering the bacterial community, makes them a potential reservoir for both new species and new antimicrobial compounds, as demonstrated in the few existing studies. Considering these facts our study aimed at: i) obtain and identify bacterial isolates sampled in three caves of Algarve, namely Vale do Telheiro, Senhora and Ibne Ammar and, ii) assess their antimicrobial activity. From the collected samples of the three caves we are able to isolate 110 bacterial isolates. After sequencing and searching for homologies in the databases it was possible to include the isolates in phyla, namely Firmicutes, Proteobacteria and Actinobacteria, in a total of 19 genera. Regarding the antimicrobial activity, the tests were performed against six test agents: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Aeromonas salmonicida ATCC 33658 and Bacillus cereus ATCC 14579. The results revealed that approximately 52% of the bacterial isolates presented antimicrobial activity against at least one test agent, and that ten bacterial isolates were able to inhibit the growth of more than four test agents. This study was the first to focus cultivable bacterial diversity in the Algarve caves and confirmed that prospection for new antimicrobial compounds in subterranean environments might be one of the strategies for fighting the problem of multi drug resistant bacteria.Recentemente, a necessidade de novas moléculas com atividade antimicrobiana aumentou devido ao contínuo aumento do número de bactérias patogénicas com resistência aos vários antibióticos disponíveis no mercado. Para colmatar esta necessidade, a comunidade científica tem realizado esforços na procura de novos microrganismos e dos seus compostos bioativos. Grande parte do esforço tem sido concentrado em ambientes com condições extremas, uma vez que nestes ambientes os microrganismos que os habitam têm de ter características muito específicas. As grutas cársicas são consideradas ambientes extremos uma vez que a ausência de luz natural impede o crescimento de plantas, fazendo com que o ambiente seja caracterizado por elevada escassez nutritiva. Juntamente com a elevada humidade e a temperatura relativamente baixa e constante, faz com os microrganismos que habitam estes ambientes se tornem altamente especializados. O facto destes habitats únicos serem pouco estudados, nomeadamente no que diz respeito à comunidade bacteriana, torna-os num potencial reservatório, tanto de novas espécies como de novos compostos antimicrobianos, tal como demonstrado nos poucos estudos existentes. Considerando estes factos, os objetivos deste estudo foram: i) obter e identificar isolados bacterianos recolhidos em três grutas do Algarve nomeadamente, as grutas do Vale do Telheiro, da Senhora e do Ibne Ammar e ii) avaliar se os mesmo têm atividade antimicrobiana. A partir das amostras recolhidas nas três grutas, foram isoladas 110 estirpes bacterianas. Após sequenciação e pesquisa de similaridades em bases de dados foi possível verificar que os isolados bacterianos pertencem a três filos, nomeadamente Firmicutes, Proteobacteria e Actinobacteria, num total de 19 géneros diferentes. Em relação à actividade antimicrobiana, os testes foram realizados contra seis agentes teste Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Aeromonas salmonicida ATCC 33658 e Bacillus cereus ATCC 14579. Os resultados revelaram que aproximadamente 52% dos isolados apresentam atividade antimicrobiana contra, pelo menos, um agente teste e que, 10 isolados foram capazes de inibir o crescimento de mais de 4 agentes teste. Este estudo foi o primeiro a focar a diversidade bacteriana cultivável de grutas do Algarve e confirmou que a prospeção de compostos antimicrobianos em ambientes subterrâneos poderá ser uma das estratégias para combater o problema da resistência aos antibióticos.2019-02-27T16:46:10Z2018-12-17T00:00:00Z2018-12-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/25453TID:202233707engVeloso, Telma Luísa Machadoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:49:29Zoai:ria.ua.pt:10773/25453Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:44.709955Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Diversity and antimicrobial potential of bacteria isolated from Algarve caves
title Diversity and antimicrobial potential of bacteria isolated from Algarve caves
spellingShingle Diversity and antimicrobial potential of bacteria isolated from Algarve caves
Veloso, Telma Luísa Machado
Caves
Diversity
Bacteria
Antimicrobial activity
title_short Diversity and antimicrobial potential of bacteria isolated from Algarve caves
title_full Diversity and antimicrobial potential of bacteria isolated from Algarve caves
title_fullStr Diversity and antimicrobial potential of bacteria isolated from Algarve caves
title_full_unstemmed Diversity and antimicrobial potential of bacteria isolated from Algarve caves
title_sort Diversity and antimicrobial potential of bacteria isolated from Algarve caves
author Veloso, Telma Luísa Machado
author_facet Veloso, Telma Luísa Machado
author_role author
dc.contributor.author.fl_str_mv Veloso, Telma Luísa Machado
dc.subject.por.fl_str_mv Caves
Diversity
Bacteria
Antimicrobial activity
topic Caves
Diversity
Bacteria
Antimicrobial activity
description Recently, the need for new molecules with antimicrobial activity increased due to a continuous the increasing number of multi drug resistant pathogenic bacteria. To fill this urging need, the scientific community has made a great investment in searching for new microorganisms and their bioactive compounds. A great part of the effort has been focused in environments with extreme conditions, since in these environments the inhabiting microorganisms must have very particular features. Karstic caves are considered extreme environments due to the nutrient scarcity caused by absence of natural light that precludes the growth of vascular green plants. In addition, the relatively low and stable temperature and the high humidity levels, force cave dwelling microorganisms to become highly specialized. The fact that these unique habitats are poorly studied, in particular when considering the bacterial community, makes them a potential reservoir for both new species and new antimicrobial compounds, as demonstrated in the few existing studies. Considering these facts our study aimed at: i) obtain and identify bacterial isolates sampled in three caves of Algarve, namely Vale do Telheiro, Senhora and Ibne Ammar and, ii) assess their antimicrobial activity. From the collected samples of the three caves we are able to isolate 110 bacterial isolates. After sequencing and searching for homologies in the databases it was possible to include the isolates in phyla, namely Firmicutes, Proteobacteria and Actinobacteria, in a total of 19 genera. Regarding the antimicrobial activity, the tests were performed against six test agents: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Aeromonas salmonicida ATCC 33658 and Bacillus cereus ATCC 14579. The results revealed that approximately 52% of the bacterial isolates presented antimicrobial activity against at least one test agent, and that ten bacterial isolates were able to inhibit the growth of more than four test agents. This study was the first to focus cultivable bacterial diversity in the Algarve caves and confirmed that prospection for new antimicrobial compounds in subterranean environments might be one of the strategies for fighting the problem of multi drug resistant bacteria.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-17T00:00:00Z
2018-12-17
2019-02-27T16:46:10Z
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