Does impedance matter when recording spikes with polytrodes?
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.3389/fnins.2018.00715 |
Resumo: | This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN). |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Does impedance matter when recording spikes with polytrodes?CoatingImpedanceMicroelectrodesNoiseSpikesNeuroscience(all)This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN).Extracellular microelectrodes have been widely used to measure brain activity, yet there are still basic questions about the requirements for a good extracellular microelectrode. One common source of confusion is how much an electrode's impedance affects the amplitude of extracellular spikes and background noise. Here we quantify the effect of an electrode's impedance on data quality in extracellular recordings, which is crucial for both the detection of spikes and their assignment to the correct neurons. This study employs commercial polytrodes containing 32 electrodes (177 μm2) arranged in a dense array. This allowed us to directly compare, side-by-side, the same extracellular signals measured by modified low impedance (∼100 kΩ) microelectrodes with unmodified high impedance (∼1 MΩ) microelectrodes. We begin with an evaluation of existing protocols to lower the impedance of the electrodes. The poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT-PSS) electrodeposition protocol is a simple, stable, and reliable method for decreasing the impedance of a microelectrode up to 10-fold. We next record in vivo using polytrodes that are modified in a 'chess board' pattern, such that the signal of one neuron is detected by multiple coated and non-coated electrodes. The performance of the coated and non-coated electrodes is then compared on measures of background noise and amplitude of the detected action potentials. If the proper recording system is used, then the impedance of a microelectrode within the range of standard polytrodes (∼0.1 to 2 MΩ) does not greatly affect data quality and spike sorting. This study should encourage neuroscientists to stop worrying about one more unknown.UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNNeto, Joana P.Baião, PedroLopes, GonçaloFrazão, JoãoNogueira, JoanaFortunato, ElviraBarquinha, PedroKampff, Adam R.2019-02-11T23:29:41Z2018-10-082018-10-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3389/fnins.2018.00715eng1662-4548PURE: 11471374http://www.scopus.com/inward/record.url?scp=85055145075&partnerID=8YFLogxKhttps://doi.org/10.3389/fnins.2018.00715info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:28:48Zoai:run.unl.pt:10362/60290Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:33:28.918433Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Does impedance matter when recording spikes with polytrodes? |
title |
Does impedance matter when recording spikes with polytrodes? |
spellingShingle |
Does impedance matter when recording spikes with polytrodes? Neto, Joana P. Coating Impedance Microelectrodes Noise Spikes Neuroscience(all) |
title_short |
Does impedance matter when recording spikes with polytrodes? |
title_full |
Does impedance matter when recording spikes with polytrodes? |
title_fullStr |
Does impedance matter when recording spikes with polytrodes? |
title_full_unstemmed |
Does impedance matter when recording spikes with polytrodes? |
title_sort |
Does impedance matter when recording spikes with polytrodes? |
author |
Neto, Joana P. |
author_facet |
Neto, Joana P. Baião, Pedro Lopes, Gonçalo Frazão, João Nogueira, Joana Fortunato, Elvira Barquinha, Pedro Kampff, Adam R. |
author_role |
author |
author2 |
Baião, Pedro Lopes, Gonçalo Frazão, João Nogueira, Joana Fortunato, Elvira Barquinha, Pedro Kampff, Adam R. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais RUN |
dc.contributor.author.fl_str_mv |
Neto, Joana P. Baião, Pedro Lopes, Gonçalo Frazão, João Nogueira, Joana Fortunato, Elvira Barquinha, Pedro Kampff, Adam R. |
dc.subject.por.fl_str_mv |
Coating Impedance Microelectrodes Noise Spikes Neuroscience(all) |
topic |
Coating Impedance Microelectrodes Noise Spikes Neuroscience(all) |
description |
This work was supported by funding from the European Union's Seventh Framework Programme (FP7/2007-2013) Grant Agreement 600925, the Bial Foundation Grant 190/12 and the FCT-MCTES Doctoral Grant SFRH/BD/76004/2011 (to JN). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-08 2018-10-08T00:00:00Z 2019-02-11T23:29:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.3389/fnins.2018.00715 |
url |
https://doi.org/10.3389/fnins.2018.00715 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1662-4548 PURE: 11471374 http://www.scopus.com/inward/record.url?scp=85055145075&partnerID=8YFLogxK https://doi.org/10.3389/fnins.2018.00715 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137956682792960 |