In silico screening of antibiotic resistance genes in a genomics library of bacteriophages

Detalhes bibliográficos
Autor(a) principal: Aguiar, Sara Catarina Leal
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/15516
Resumo: Antibiotic resistance (AR) is a worldwide concern that threatens the effective treatment of infectionsusing antibiotics (1-5). It is known that AR is often mobile between bacteria of different taxonomic and ecological groups by either transformation, conjugation or tranduction (6,7). Here is address the controversial dissemination of AR through the mechanism of transduction by determining the presence of antibiotic resistance genes in bacteriphages genomes through an in silico approach. As such this dissertation presentes the sreening of 4789 antibiotic resistance genes (ARGs) in all 2051 bacteriophages genomes of the order Caudovirales available online using an in silico method of choise, and further intends to discuss the chalanges in the choice as also discuss the pros and limitations of the method chosen. Results determinated the presence of 32 different antibiotic resistance gene famalies in 16 different phage hosts at a 99% confidence interval. Although the results showed strong diversity among the phage genomes where ARGs were found, the impact oh the spread of AR through the transduction mechanism remains unclear. In order to address these questions, further future work can be carried out, although the releance might be also questioned.
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spelling In silico screening of antibiotic resistance genes in a genomics library of bacteriophagesResistência a antibióticosBacteriófagosBioinformáticaTransduçãoAntibiotic resistanceBacteriophagesBioinformaticsTransductionAntibiotic resistance (AR) is a worldwide concern that threatens the effective treatment of infectionsusing antibiotics (1-5). It is known that AR is often mobile between bacteria of different taxonomic and ecological groups by either transformation, conjugation or tranduction (6,7). Here is address the controversial dissemination of AR through the mechanism of transduction by determining the presence of antibiotic resistance genes in bacteriphages genomes through an in silico approach. As such this dissertation presentes the sreening of 4789 antibiotic resistance genes (ARGs) in all 2051 bacteriophages genomes of the order Caudovirales available online using an in silico method of choise, and further intends to discuss the chalanges in the choice as also discuss the pros and limitations of the method chosen. Results determinated the presence of 32 different antibiotic resistance gene famalies in 16 different phage hosts at a 99% confidence interval. Although the results showed strong diversity among the phage genomes where ARGs were found, the impact oh the spread of AR through the transduction mechanism remains unclear. In order to address these questions, further future work can be carried out, although the releance might be also questioned.A resistência a antibióticos é uma preocupação mundial que ameaça o tratamento eficaz de infeções usando antibióticos. Atualmente, as bactérias podem se tornar resistentes a quase todos os antibióticos (1-5). Sabe-se que a resistência a antibióticos é frequentemente móvel entre bactérias de diferentes grupos taxonómicos e ecológicos por transformação, conjugação ou transdução (6,7). Aqui é abordada a controversa disseminação de resistência a antibióticos através do mecanismo de transdução. determinando a presença de genes de resistência a antibióticos em genomas de bacteriófagos através de uma abordagem in silico. Como tal, esta dissertação apresenta um screening de 4789 genes de resistência a antibióticos em todos os 2051 genomas de bacteriófagos da ordem de Caudovirales disponíveis on-line usando um método in silico previamente escolhido, e pretende discutir os desafios na escolha como também discutir os prós e limitações do método escolhido. Os resultados determinam a presença de 32 diferentes famílias de genes de resistência a antibióticos em 16 bacteriófagos diferentes a um intervalo de confiança de 99%. Embora os resultados mostrem uma forte diversidade entre os genomas de fagos onde foram encontrados genes de resistência a antibióticos, o impacto da disseminação de resistência a antibióticos através do mecanismo de transdução permanece incerto. Para abordar essas questões, trabalhos futuros podem ser realizados, embora a relevância possa ser questionada.Fernandes, RubenBaylina, PilarSá, VitorRepositório Científico do Instituto Politécnico do PortoAguiar, Sara Catarina Leal2020-02-21T14:32:09Z2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/15516TID:202445380engmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:59:38Zoai:recipp.ipp.pt:10400.22/15516Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:35:13.694231Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
title In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
spellingShingle In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
Aguiar, Sara Catarina Leal
Resistência a antibióticos
Bacteriófagos
Bioinformática
Transdução
Antibiotic resistance
Bacteriophages
Bioinformatics
Transduction
title_short In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
title_full In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
title_fullStr In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
title_full_unstemmed In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
title_sort In silico screening of antibiotic resistance genes in a genomics library of bacteriophages
author Aguiar, Sara Catarina Leal
author_facet Aguiar, Sara Catarina Leal
author_role author
dc.contributor.none.fl_str_mv Fernandes, Ruben
Baylina, Pilar
Sá, Vitor
Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Aguiar, Sara Catarina Leal
dc.subject.por.fl_str_mv Resistência a antibióticos
Bacteriófagos
Bioinformática
Transdução
Antibiotic resistance
Bacteriophages
Bioinformatics
Transduction
topic Resistência a antibióticos
Bacteriófagos
Bioinformática
Transdução
Antibiotic resistance
Bacteriophages
Bioinformatics
Transduction
description Antibiotic resistance (AR) is a worldwide concern that threatens the effective treatment of infectionsusing antibiotics (1-5). It is known that AR is often mobile between bacteria of different taxonomic and ecological groups by either transformation, conjugation or tranduction (6,7). Here is address the controversial dissemination of AR through the mechanism of transduction by determining the presence of antibiotic resistance genes in bacteriphages genomes through an in silico approach. As such this dissertation presentes the sreening of 4789 antibiotic resistance genes (ARGs) in all 2051 bacteriophages genomes of the order Caudovirales available online using an in silico method of choise, and further intends to discuss the chalanges in the choice as also discuss the pros and limitations of the method chosen. Results determinated the presence of 32 different antibiotic resistance gene famalies in 16 different phage hosts at a 99% confidence interval. Although the results showed strong diversity among the phage genomes where ARGs were found, the impact oh the spread of AR through the transduction mechanism remains unclear. In order to address these questions, further future work can be carried out, although the releance might be also questioned.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2019-12-01T00:00:00Z
2020-02-21T14:32:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/15516
TID:202445380
url http://hdl.handle.net/10400.22/15516
identifier_str_mv TID:202445380
dc.language.iso.fl_str_mv eng
language eng
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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