Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/117265 |
Resumo: | Antibodies are known to play an important role in the control of malaria infection. However, they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on the expression of circumsporozoite protein gene (csp) was investigated. Plasmodium falciparum 3D7 in vitro cultures were submitted to: i) anti- circumsporozoite protein monoclonal antibody (anti-CSP-mAb) [1μg/ml, 0.1μg/ml, 0.01μg/ml and 0.001μg/ml] and ii) purified IgG Fab fragment from a pool of malaria patients [1mg/ml and 1μg/ml]; and compared to control cultures. After 24h the number of ring infected erythrocytes was determined in order to calculate invasion efficacy. At 48h culture supernatant was collected, and the amount of circumsporozoite protein determined by ELISA, parasitaemia was calculated and cells were processed for RNA preparation. Expression of csp gene was quantified using Real time RT-PCR. There was an increase in parasite growth when treated with lower anti-CSP-mAb concentration, which was associated with lower csp expression, while 1μg/ml anti-CSP-mAb treatment presented a growth inhibitory effect accompanied by high csp expression. |
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Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein geneCircumsporozoite proteinErythrocyte invasionPlasmodium falciparumImmunologyParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingAntibodies are known to play an important role in the control of malaria infection. However, they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on the expression of circumsporozoite protein gene (csp) was investigated. Plasmodium falciparum 3D7 in vitro cultures were submitted to: i) anti- circumsporozoite protein monoclonal antibody (anti-CSP-mAb) [1μg/ml, 0.1μg/ml, 0.01μg/ml and 0.001μg/ml] and ii) purified IgG Fab fragment from a pool of malaria patients [1mg/ml and 1μg/ml]; and compared to control cultures. After 24h the number of ring infected erythrocytes was determined in order to calculate invasion efficacy. At 48h culture supernatant was collected, and the amount of circumsporozoite protein determined by ELISA, parasitaemia was calculated and cells were processed for RNA preparation. Expression of csp gene was quantified using Real time RT-PCR. There was an increase in parasite growth when treated with lower anti-CSP-mAb concentration, which was associated with lower csp expression, while 1μg/ml anti-CSP-mAb treatment presented a growth inhibitory effect accompanied by high csp expression.Instituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)RUNJesuíno, B. S.Casimiro, C.Do Rosário, V. E.Silveira, H.2021-05-06T22:43:40Z2006-01-012006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttp://hdl.handle.net/10362/117265eng1449-1907PURE: 26802935https://doi.org/10.7150/ijms.3.7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:24Zoai:run.unl.pt:10362/117265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:34.359233Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
title |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
spellingShingle |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene Jesuíno, B. S. Circumsporozoite protein Erythrocyte invasion Plasmodium falciparum Immunology Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
title_full |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
title_fullStr |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
title_full_unstemmed |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
title_sort |
Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene |
author |
Jesuíno, B. S. |
author_facet |
Jesuíno, B. S. Casimiro, C. Do Rosário, V. E. Silveira, H. |
author_role |
author |
author2 |
Casimiro, C. Do Rosário, V. E. Silveira, H. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) RUN |
dc.contributor.author.fl_str_mv |
Jesuíno, B. S. Casimiro, C. Do Rosário, V. E. Silveira, H. |
dc.subject.por.fl_str_mv |
Circumsporozoite protein Erythrocyte invasion Plasmodium falciparum Immunology Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Circumsporozoite protein Erythrocyte invasion Plasmodium falciparum Immunology Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Antibodies are known to play an important role in the control of malaria infection. However, they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on the expression of circumsporozoite protein gene (csp) was investigated. Plasmodium falciparum 3D7 in vitro cultures were submitted to: i) anti- circumsporozoite protein monoclonal antibody (anti-CSP-mAb) [1μg/ml, 0.1μg/ml, 0.01μg/ml and 0.001μg/ml] and ii) purified IgG Fab fragment from a pool of malaria patients [1mg/ml and 1μg/ml]; and compared to control cultures. After 24h the number of ring infected erythrocytes was determined in order to calculate invasion efficacy. At 48h culture supernatant was collected, and the amount of circumsporozoite protein determined by ELISA, parasitaemia was calculated and cells were processed for RNA preparation. Expression of csp gene was quantified using Real time RT-PCR. There was an increase in parasite growth when treated with lower anti-CSP-mAb concentration, which was associated with lower csp expression, while 1μg/ml anti-CSP-mAb treatment presented a growth inhibitory effect accompanied by high csp expression. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2006-01-01T00:00:00Z 2021-05-06T22:43:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/117265 |
url |
http://hdl.handle.net/10362/117265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1449-1907 PURE: 26802935 https://doi.org/10.7150/ijms.3.7 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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