High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/151075 |
Resumo: | This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
| id |
RCAP_fceb6f3872a0bf5108099c45788e8c85 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/151075 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital WastewaterEffluentsEMRSA-15HospitalMRSAStaphylococcus aureusWastewatersMicrobiologyMicrobiology (medical)VirologyThis work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Hospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the antimicrobial resistance and genetic lineages. A total of 96 wastewater samples were collected over six months. The water was filtered, and the filtration membrane was immersed in BHI broth supplemented with 6.5% of NaCl and incubated. The inoculum was streaked in ORSAB agar plates for MRSA isolation. The isolates susceptibility testing was performed against 14 antimicrobial agents. The presence of resistance and virulence genes was accessed by PCR. Molecular typing was performed in all isolates. From the 96 samples, 28 (29.2%) were MRSA-positive. Most isolates had a multidrug-resistant profile and carried the mecA, blaZ, aac(6′)-Ie-aph(2′′)-Ia, aph(3′)-IIIa, ermA, ermB, ermC, tetL, tetM, dfrA dfrG and catpC221 genes. Most of the isolates were ascribed to the immune evasion cluster (IEC) type B. The isolates belonged to ST22-IV, ST8-IV and ST105-II and spa-types t747, t1302, t19963, t6966, t020, t008 and tOur study shows that MRSA can be found over time in hospital wastewater. The wastewater treatment processes can reduce the MRSA load. The great majority of the isolates belonged to ST22 and spa-type t747 which suggests the fitness of these genetic lineages in hospital effluents.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSilva, VanessaRibeiro, JéssicaRocha, JaquelineManaia, Célia M.Silva, AdrianaPereira, José EduardoMaltez, LuísCapelo, José LuisIgrejas, GilbertoPoeta, Patrícia2023-03-22T22:28:48Z2022-01-112022-01-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/151075engSilva, V., Ribeiro, J., Rocha, J., Manaia, C. M., Silva, A., Pereira, J. E., Maltez, L., Capelo, J. L., Igrejas, G., & Poeta, P. (2022). High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater. Microorganisms, 10(1), [147]. https://doi.org/10.3390/microorganisms100101472076-2607PURE: 56611750https://doi.org/10.3390/microorganisms10010147info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:33:31Zoai:run.unl.pt:10362/151075Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:26.912041Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| title |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| spellingShingle |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater Silva, Vanessa Effluents EMRSA-15 Hospital MRSA Staphylococcus aureus Wastewaters Microbiology Microbiology (medical) Virology |
| title_short |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| title_full |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| title_fullStr |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| title_full_unstemmed |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| title_sort |
High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater |
| author |
Silva, Vanessa |
| author_facet |
Silva, Vanessa Ribeiro, Jéssica Rocha, Jaqueline Manaia, Célia M. Silva, Adriana Pereira, José Eduardo Maltez, Luís Capelo, José Luis Igrejas, Gilberto Poeta, Patrícia |
| author_role |
author |
| author2 |
Ribeiro, Jéssica Rocha, Jaqueline Manaia, Célia M. Silva, Adriana Pereira, José Eduardo Maltez, Luís Capelo, José Luis Igrejas, Gilberto Poeta, Patrícia |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Silva, Vanessa Ribeiro, Jéssica Rocha, Jaqueline Manaia, Célia M. Silva, Adriana Pereira, José Eduardo Maltez, Luís Capelo, José Luis Igrejas, Gilberto Poeta, Patrícia |
| dc.subject.por.fl_str_mv |
Effluents EMRSA-15 Hospital MRSA Staphylococcus aureus Wastewaters Microbiology Microbiology (medical) Virology |
| topic |
Effluents EMRSA-15 Hospital MRSA Staphylococcus aureus Wastewaters Microbiology Microbiology (medical) Virology |
| description |
This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-01-11 2022-01-11T00:00:00Z 2023-03-22T22:28:48Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/151075 |
| url |
http://hdl.handle.net/10362/151075 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Silva, V., Ribeiro, J., Rocha, J., Manaia, C. M., Silva, A., Pereira, J. E., Maltez, L., Capelo, J. L., Igrejas, G., & Poeta, P. (2022). High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater. Microorganisms, 10(1), [147]. https://doi.org/10.3390/microorganisms10010147 2076-2607 PURE: 56611750 https://doi.org/10.3390/microorganisms10010147 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
10 application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138133064810496 |