Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape

Detalhes bibliográficos
Autor(a) principal: Schuster, Dominik
Data de Publicação: 2017
Outros Autores: Rickmeyer, Jasmin, Gajdiss, Mike, Thye, Thorsten, Lorenzen, Stephan, Reif, Marion, Josten, Michaele, Melo, Luís D. R., Schmithausen, Ricarda M., Liégeois, Florian, Sahl, Hans-Georg, Gonzalez, Jean-Paul J., Nagel, Michael, Bierbaum, Gabriele
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/44722
Resumo: The species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice.
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spelling Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great apeStaphylococcus argenteusStaphylococcus aureusStaphylococcus schweitzeriGorilla gorilla ssp. gorillaMALDI-TOF MSVirulence determinantsScience & TechnologyThe species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice.This work was supported by a grant of the Deutsches Zentrum für Infektionsforschung (DZIF) to Gabriele Bierbaum (TTU 08.902) and the Fundação para a Ciência e a Tecnologia (FCT) to Luís D.R. Melo (SFRH/BD/66166/2009) as well as by the BONFOR funds of the Medical Faculty of the University of Bonn and funds of the Centre International de Recherches Médicales de Franceville (CIRMF) and the Institut de Recherche pour le Développement (IRD).ElsevierUniversidade do MinhoSchuster, DominikRickmeyer, JasminGajdiss, MikeThye, ThorstenLorenzen, StephanReif, MarionJosten, MichaeleMelo, Luís D. R.Schmithausen, Ricarda M.Liégeois, FlorianSahl, Hans-GeorgGonzalez, Jean-Paul J.Nagel, MichaelBierbaum, Gabriele20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44722engSchuster, Dominik; Rickmeyer, Jasmin; Gajdiss, Mike; Thye, Thorsten; Lorenzen, Stephan; Reif, Marion; Josten, Michaele; Melo, Luís D. R.; Schmithausen, Ricarda M.; Liégeois, Florian; Sahl, Hans-Georg; Gonzalez, Jean-Paul J.; Nagel, Michael; Bierbaum, Gabriele, Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape. International Journal of Medical Microbiology, 307(1), 57-63, 20171618-06071438-422110.1016/j.ijmm.2016.11.00327931949http://www.journals.elsevier.com/international-journal-of-medical-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:14Zoai:repositorium.sdum.uminho.pt:1822/44722Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:26.171377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
title Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
spellingShingle Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
Schuster, Dominik
Staphylococcus argenteus
Staphylococcus aureus
Staphylococcus schweitzeri
Gorilla gorilla ssp. gorilla
MALDI-TOF MS
Virulence determinants
Science & Technology
title_short Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
title_full Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
title_fullStr Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
title_full_unstemmed Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
title_sort Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
author Schuster, Dominik
author_facet Schuster, Dominik
Rickmeyer, Jasmin
Gajdiss, Mike
Thye, Thorsten
Lorenzen, Stephan
Reif, Marion
Josten, Michaele
Melo, Luís D. R.
Schmithausen, Ricarda M.
Liégeois, Florian
Sahl, Hans-Georg
Gonzalez, Jean-Paul J.
Nagel, Michael
Bierbaum, Gabriele
author_role author
author2 Rickmeyer, Jasmin
Gajdiss, Mike
Thye, Thorsten
Lorenzen, Stephan
Reif, Marion
Josten, Michaele
Melo, Luís D. R.
Schmithausen, Ricarda M.
Liégeois, Florian
Sahl, Hans-Georg
Gonzalez, Jean-Paul J.
Nagel, Michael
Bierbaum, Gabriele
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Schuster, Dominik
Rickmeyer, Jasmin
Gajdiss, Mike
Thye, Thorsten
Lorenzen, Stephan
Reif, Marion
Josten, Michaele
Melo, Luís D. R.
Schmithausen, Ricarda M.
Liégeois, Florian
Sahl, Hans-Georg
Gonzalez, Jean-Paul J.
Nagel, Michael
Bierbaum, Gabriele
dc.subject.por.fl_str_mv Staphylococcus argenteus
Staphylococcus aureus
Staphylococcus schweitzeri
Gorilla gorilla ssp. gorilla
MALDI-TOF MS
Virulence determinants
Science & Technology
topic Staphylococcus argenteus
Staphylococcus aureus
Staphylococcus schweitzeri
Gorilla gorilla ssp. gorilla
MALDI-TOF MS
Virulence determinants
Science & Technology
description The species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/44722
url http://hdl.handle.net/1822/44722
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Schuster, Dominik; Rickmeyer, Jasmin; Gajdiss, Mike; Thye, Thorsten; Lorenzen, Stephan; Reif, Marion; Josten, Michaele; Melo, Luís D. R.; Schmithausen, Ricarda M.; Liégeois, Florian; Sahl, Hans-Georg; Gonzalez, Jean-Paul J.; Nagel, Michael; Bierbaum, Gabriele, Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape. International Journal of Medical Microbiology, 307(1), 57-63, 2017
1618-0607
1438-4221
10.1016/j.ijmm.2016.11.003
27931949
http://www.journals.elsevier.com/international-journal-of-medical-microbiology/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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